scholarly journals Mitochondrial-derived reactive oxygen species (ROS) play a causal role in aging-related intervertebral disc degeneration

2013 ◽  
Vol 31 (7) ◽  
pp. 1150-1157 ◽  
Author(s):  
Luigi A. Nasto ◽  
Andria R. Robinson ◽  
Kevin Ngo ◽  
Cheryl L. Clauson ◽  
Qing Dong ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Reactive Oxygen ◽  
Causal Role ◽  
Oxygen Species

scholarly journals Excessive reactive oxygen species are therapeutic targets for intervertebral disc degeneration

2015 ◽  
Vol 17 (1) ◽  
Author(s):  
Satoshi Suzuki ◽  
Nobuyuki Fujita ◽  
Naobumi Hosogane ◽  
Kota Watanabe ◽  
Ken Ishii ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Therapeutic Targets ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals Reactive Oxygen Species Mediate Low Back Pain by Upregulating Substance P in Intervertebral Disc Degeneration

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Jiancheng Zheng ◽  
Jian Zhang ◽  
Xingkai Zhang ◽  
Zhiping Guo ◽  
Wenjian Wu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Low Back Pain ◽  
Back Pain ◽  
Substance P ◽  
Intervertebral Disc ◽  
Reactive Oxygen ◽  
Alternative Methods ◽  
Low Back ◽  
Oxygen Species ◽  
Ivd Degeneration

Reactive oxygen species (ROS) are thought to have a strong correlation with a number of intervertebral disc (IVD) diseases. Here, we aimed to determine whether ROS represent an etiology of low back pain (LBP) during IVD degeneration. Thirty degenerated intervertebral disc samples were obtained from patients, and ROS levels were quantified using dihydroethidium (DHE) staining. The results suggested a significant correlation between the ROS level and the severity of LBP. Subsequently, a puncture-induced LBP model was established in rats, and ROS levels significantly increased compared with those in the sham surgery group, accompanied with severe puncture-induced IVD degeneration. In addition, when ROS levels were increased by H2O2 administration or decreased by NAC treatment, the rats showed increased or decreased LBP, respectively. Based on this evidence, we further determined that stimulation with H2O2 in nucleus pulposus cells (NPCs) in vivo or in vitro resulted in upregulation of substance P (SP), a peptide thought to be involved in the synaptic transmission of pain, and that the severity of LBP decreased when SP levels were increased by exogenous SP administration or neutralized via aprepitant treatment in the IVDs of rats. In conclusion, ROS are primary inducers of LBP based on clinical and animal data, and the mechanism involves ROS stimulation of NPCs to secrete SP, which is a critical neurotransmitter peptide, to promote LBP in IVDs. Therefore, reducing the level of ROS with specific drugs and inhibiting SP may be alternative methods to treat LBP in the clinic.

scholarly journals Aspirin-Mediated Attenuation of Intervertebral Disc Degeneration by Ameliorating Reactive Oxygen Species In Vivo and In Vitro

2019 ◽  
Vol 2019 ◽  
pp. 1-20 ◽  
Author(s):  
Yu Liu ◽  
Jiayi Lin ◽  
Xiexing Wu ◽  
Xiaobin Guo ◽  
Houyi Sun ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Back Pain ◽  
Intervertebral Disc ◽  
Signaling Pathway ◽  
Nucleus Pulposus ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Ampk Signaling

Intervertebral disc (IVD) degeneration (IDD) is a major cause of low back pain. The pathogenesis of IDD is associated with the disturbance of reactive oxygen species (ROS) equilibrium, inflammation, and matrix loss. Aspirin is a nonsteroidal anti-inflammatory drug that effectively inhibits inflammation and oxidative stress and has been widely used for the treatment of back pain. Therefore, we hypothesize that aspirin reverses the IDD process via antioxidative and anti-inflammatory effects on the AMPK signaling pathway. In vitro, aspirin diminished cellular oxygen free radicals (ROS, nitric oxide (NO)) and inflammatory cytokines (interleukin- (IL-) 1β and IL-6 and tumor necrosis factor alpha (TNF-α)) induced by lipopolysaccharides (LPS) in nucleus pulposus cells (NPCs). We found that aspirin preserved the extracellular matrix (ECM) content of collagen type II (COL2) and aggrecan while inhibiting the expression of matrix-degenerating enzymes, including matrix metalloproteinase 3 and 13 (MMP-3 and MMP-13) and A disintegrin and metalloproteinase with thrombospondin motifs 4 and 5 (ADAMTS-4, ADAMTS-5). Aspirin significantly promoted the ratios of p-AMPK to AMPK and p-ACC to ACC expression in NPCs. Furthermore, pretreatment with the AMPK inhibitor compound C abrogated the antioxidant effects of aspirin. In vivo, an IDD model was established in Sprague-Dawley rats via percutaneous disc puncture with the 20-gauge needle on levels 8-9 and 9-10 of the coccygeal vertebrae. Imaging assessment showed that after aspirin treatment, improvements in disc height index (DHI) ranged from 1.22-fold to 1.54-fold and nucleus pulposus signal strength improved from 1.26-fold to 1.33-fold. Histological analysis showed that aspirin treatment prevented the loss of COL2 and decreased MMP-3 and MMP-13, inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), IL-1β, and TNF-α expression in the IVD tissues. These results suggest that treatment with aspirin could reverse the IDD process via the AMPK signaling pathway, which provides new insights into the potential clinical applications of aspirin, particularly for IDD treatment.

scholarly journals A thermosensitive, reactive oxygen species-responsive, MR409-encapsulated hydrogel ameliorates disc degeneration in rats by inhibiting the secretory autophagy pathway

Theranostics ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 147-163
Author(s):  
Qiangqiang Zheng ◽  
Haotian Shen ◽  
Zongrui Tong ◽  
Linxiang Cheng ◽  
Yuzi Xu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Disc Degeneration ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Autophagy Pathway ◽  
Secretory Autophagy

Reactive oxygen species have a causal role in multiple forms of insulin resistance

Nature ◽  
2006 ◽  
Vol 440 (7086) ◽  
pp. 944-948 ◽  
Author(s):  
Nicholas Houstis ◽  
Evan D. Rosen ◽  
Eric S. Lander
Keyword(s):  
Insulin Resistance ◽  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Causal Role ◽  
Oxygen Species ◽  
Multiple Forms

Mitochondria as a source of reactive oxygen species

2009 ◽  
pp. c3 ◽  
Author(s):  
Helena M. Cochemé ◽  
Michael P. Murphy
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species
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