Pharmacological Disrupting of P-eIF2α/ATF4/Ihh Signaling Protects Intervertebral Disc Degeneration Via Reducing the Reactive Oxygen Species and Apoptosis in Nucleus Pulposus Cells

2021 ◽  
Author(s):  
Zhanyang Qian ◽  
You Li ◽  
Renyi Kong ◽  
Junping Bao ◽  
Xin Peng ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Nucleus Pulposus Cells

scholarly journals Mitochondrial-derived reactive oxygen species (ROS) play a causal role in aging-related intervertebral disc degeneration

2013 ◽  
Vol 31 (7) ◽  
pp. 1150-1157 ◽  
Author(s):  
Luigi A. Nasto ◽  
Andria R. Robinson ◽  
Kevin Ngo ◽  
Cheryl L. Clauson ◽  
Qing Dong ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Reactive Oxygen ◽  
Causal Role ◽  
Oxygen Species

scholarly journals Excessive reactive oxygen species are therapeutic targets for intervertebral disc degeneration

2015 ◽  
Vol 17 (1) ◽  
Author(s):  
Satoshi Suzuki ◽  
Nobuyuki Fujita ◽  
Naobumi Hosogane ◽  
Kota Watanabe ◽  
Ken Ishii ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Therapeutic Targets ◽  
Reactive Oxygen ◽  
Oxygen Species

Intervention of rAAV-hTERT-Transducted Nucleus Pulposus Cells in Early Stage of Intervertebral Disc Degeneration: A Study in Canine Model

2015 ◽  
Vol 21 (15-16) ◽  
pp. 2186-2194 ◽  
Author(s):  
Zhiyuan Shi ◽  
Tao Gu ◽  
Hongkui Xin ◽  
Jianhong Wu ◽  
Cheng Xu ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Early Stage ◽  
Canine Model ◽  
Nucleus Pulposus Cells

scholarly journals LOX Alleviates Rat Intervertebral Disc Degeneration Through ECM Improvement and Anti-Apoptotic Protection in Nucleus Pulposus Cells

2019 ◽  
Vol 16 (S2) ◽  
pp. 77-77
Author(s):  
Runze Zhao ◽  
Tingting Xia ◽  
Mengyue Wang ◽  
Fan Feng ◽  
Wanqian Liu ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Nucleus Pulposus Cells

scholarly journals Ligustrazine Prevents Intervertebral Disc Degeneration via Suppression of Aberrant TGFβ Activation in Nucleus Pulposus Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Shufen Liu ◽  
Yuhao Cheng ◽  
Yuqi Tan ◽  
Jingcheng Dong ◽  
Qin Bian
Keyword(s):  
Growth Factor ◽  
Mouse Model ◽  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Ex Vivo ◽  
Tissue Growth ◽  
Nucleus Pulposus Cells

Objectives. Aberrant transforming growth factor β (TGFβ) activation is detrimental to both nucleus pulposus (NP) cells and cartilage endplates (CEPs), which can lead to intervertebral disc degeneration (IDD). Ligustrazine (LIG) reduces the expression of inflammatory factors and TGFβ1 in hypertrophic CEP to prevent IDD. In this study, we investigate the effects of LIG on NP cells and the TGFβ signaling. Design. LIG was injected to the lumbar spinal instability (LSI) mouse model. The effect of LIG was evaluated by intervertebral disc (IVD) score in the LSI mouse model. The expression of activated TGFβ was examined using immunostaining with pSmad2/3 antibody. The upright posture (UP) rat model was also treated and evaluated in the same manner to assess the effect of LIG. In ex vivo study, IVDs from four-week old mice were isolated and treated with 10−5, 10−6, and 10−7 M of LIG. We used western blot to detect activated TGFβ expression. TGFβ-treated human nucleus pulposus cells (HNPCs) were cotreated with optimized dose of LIG in vitro. Immunofluorescence staining was performed to determine pSmad2/3, connective tissue growth factor (CCN2), and aggrecan (ACAN) expression levels. Results. IVD score and the percentage of pSmad2/3+ NP cells were low in LIG-treated LSI mice in comparison with LSI mice, but close to the levels in the Sham group. Similarly, LIG reduced the overexpression of TGFβ1 in NP cells. The inhibitory effect of LIG was dose dependent. A dose of 10−5 M LIG not only strongly attenuated Smad2/3 phosphorylation in TGFβ-treated IVD ex vivo but also suppressed pSmad2/3, CCN2, and ACAN expression in TGFβ-treated NP cells in vitro. Conclusions. LIG prevents IDD via suppression of TGFβ overactivation in NP cells.

scholarly journals TGF-β Initiates β-Catenin-Mediated CTGF Secretory Pathway in Old Bovine Nucleus Pulposus Cells: A Potential Mechanism for Intervertebral Disc Degeneration

JBMR Plus ◽  
2018 ◽  
Vol 3 (2) ◽  
pp. e10069 ◽  
Author(s):  
Qiuqian Wu ◽  
Chun Mathers ◽  
Ernest W Wang ◽  
Sen Sheng ◽  
David Wenkert ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Secretory Pathway ◽  
Potential Mechanism ◽  
Nucleus Pulposus Cells

scholarly journals Acacetin Alleviates Inflammation and Matrix Degradation in Nucleus Pulposus Cells and Ameliorates Intervertebral Disc Degeneration in vivo

2020 ◽  
Vol Volume 14 ◽  
pp. 4801-4813
Author(s):  
Hao Wang ◽  
Zengxin Jiang ◽  
Zhiying Pang ◽  
Tianyao Zhou ◽  
Yutong Gu
Keyword(s):  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Matrix Degradation ◽  
Nucleus Pulposus Cells
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