Targeted gene delivery to skin cells in vivo: A comparative study of liposomes and polymers as delivery vehicles

It requires multistep synthesis and conjugation processes to incorporate multifunctionalities into a polyplex gene vehicle to overcome numerous hurdles during gene delivery. Here, we describe a supramolecular platform to precisely control, screen, and optimize molecular architectures of siRNA targeted delivery vehicles, which is based on rationally designed host-guest complexation between a β-cyclodextrin–based cationic host polymer and a library of guest polymers with various PEG shape and size, and various density of ligands. The host polymer is responsible to load/unload siRNA, while the guest polymer is responsible to shield the vehicles from nonspecific cellular uptake, to prolong their circulation time, and to target tumor cells. A series of precisely controlled molecular architectures through a simple assembly process allow for a rapid optimization of siRNA delivery vehicles in vitro and in vivo for therapeutic siRNA-Bcl2 delivery and tumor therapy, indicating the platform is a powerful screening tool for targeted gene delivery vehicles.

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Novel Mannan-PEG-PE Modified Bioadhesive PLGA Nanoparticles for Targeted Gene Delivery

Journal of Nanomaterials ◽

10.1155/2012/981670 ◽

2012 ◽

Vol 2012 ◽

pp. 1-9 ◽

Cited By ~ 2

Author(s):

Guicun Wu ◽

Fang Zhou ◽

Linfu Ge ◽

Ximin Liu ◽

Fansheng Kong

Keyword(s):

Gene Delivery ◽

Transfection Efficiency ◽

Polymeric Nanoparticles ◽

Plga Nanoparticles ◽

Gene Delivery System ◽

Targeted Gene Delivery ◽

Delivery Vehicles ◽

Modification Rate

Purpose. Biodegradable polymeric nanoparticles have been used frequently as gene delivery vehicles. The aim of this study is to modify bioadhesive PLGA nanoparticles with novel synthetic mannan-PEG-PE (MN-PEG-PE) to obtain active targeted gene delivery system.Methods. Mannan-PEG-PE ligands were synthesized and modified onto the NPs/pEGFP complexes. The modification rate was optimized, and the characteristics of the vehicle were evaluated. Then, the modified vectors were intravenous delivered to rats, andin vivotargeting behavior of MN-PEG-PE modified PLGA nanoparticles/pEGFP complexes (MN-PEG-PE-NPs/pEGFP) in liver macrophages was investigated.Results. MN-PEG-PE-NPs/pEGFP displayed remarkably higher transfection efficiencies than nonmodified NPs/pEGFP bothin vitroandin vivo.Conclusions. Mannan containing targeting ligands could significantly improve the transfection efficiency of the carriers. MN-PEG-PE modified vectors very useful in targeted gene delivery.

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Phase I/II and Phase II Studies of Targeted Gene Delivery In Vivo: Intravenous Rexin-G for Chemotherapy-resistant Sarcoma and Osteosarcoma

Molecular Therapy ◽

10.1038/mt.2009.126 ◽

2009 ◽

Vol 17 (9) ◽

pp. 1651-1657 ◽

Cited By ~ 38

Author(s):

Sant P Chawla ◽

Victoria S Chua ◽

Lita Fernandez ◽

Doris Quon ◽

Andreh Saralou ◽

...

Keyword(s):

Gene Delivery ◽

Phase I ◽

Phase Ii ◽

Targeted Gene Delivery ◽

Phase Ii Studies

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Asialoglycoprotein receptor targeted gene delivery using galactosylated polyethylenimine-graft-poly(ethylene glycol): In vitro and in vivo studies

Journal of Controlled Release ◽

10.1016/j.jconrel.2005.09.001 ◽

2005 ◽

Vol 108 (2-3) ◽

pp. 557-567 ◽

Cited By ~ 45

Author(s):

Eun-Mi Kim ◽

Hwan-Jeong Jeong ◽

In-Kyu Park ◽

Chong-Su Cho ◽

Hyung-Bae Moon ◽

...

Keyword(s):

Gene Delivery ◽

Ethylene Glycol ◽

Asialoglycoprotein Receptor ◽

In Vivo Studies ◽

Poly Ethylene Glycol ◽

Targeted Gene Delivery ◽

Poly Ethylene

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Receptor-Mediated Gene Delivery Using Chitosan Derivatives In Vitro and In Vivo

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.342-343.449 ◽

2007 ◽

Vol 342-343 ◽

pp. 449-452 ◽

Cited By ~ 1

Author(s):

Tae Hee Kim ◽

Hua Jin ◽

Hyun Woo Kim ◽

Myung Haing Cho ◽

Jae Woon Nah ◽

...

Keyword(s):

Gene Delivery ◽

Delivery System ◽

Transfection Efficiency ◽

Viral Gene ◽

Gene Delivery System ◽

Targeted Gene Delivery ◽

Cell Specificity ◽

Selective Delivery

The key strategy for the advancement of gene therapy is the development of an efficient targeted gene delivery system into cells. The targeted gene delivery system is especially important in non-viral gene transfer which shows the relatively low transfection efficiency. It also opens the possibility of selective delivery of therapeutic plasmids to specific tissues. Chitosan has been considered to be a good candidate for gene delivery system, since it is already known as a biocompatible, biodegradable, and low toxic material with high cationic potential. However, low specificity and low transfection efficiency of chitosan need to be overcome prior to clinical trial. In this study, we focused on the chemical modification of chitosan for enhancement of cell specificity and transfection efficiency. Also, the potential of clinical application was investigated.

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