Antitumor Agents XLI: Effects of Eupaformosanin on Nucleic Acid, Protein, and Anaerobic and Aerobic Glycolytic Metabolism of Ehrlich Ascites Cells

1980 ◽  
Vol 69 (3) ◽  
pp. 294-297 ◽  
Author(s):  
I.H. Hall ◽  
K.H. Lee ◽  
W.L. Williams ◽  
T. Kimura ◽  
T. Hirayama
Keyword(s):  
Nucleic Acid ◽  
Antitumor Agents ◽  
Glycolytic Metabolism ◽  
Acid Protein ◽  
Ehrlich Ascites ◽  
Ehrlich Ascites Cells

scholarly journals Photochemical cross-linking of protein and DNA in chromatin. Synthesis and application of a photosensitive cleavable derivative of 9-aminoacridine with two photoprobes connected through a disulphide-containing linker

1984 ◽  
Vol 223 (2) ◽  
pp. 519-526 ◽  
Author(s):  
P E Nielsen ◽  
J B Hansen ◽  
O Buchardt
Keyword(s):  
Nucleic Acid ◽  
Visible Light ◽  
Cross Linking ◽  
Disulphide Bridge ◽  
Long Wavelength ◽  
Ehrlich Ascites ◽  
Ehrlich Ascites Cells ◽  
Cross Links ◽  
Protein Nucleic Acid ◽  
Nucleic Acid Interactions

A novel cleavable photo-cross-linking reagent, N-(2-methoxy-6-azidoacridin-9-yl)-N'-(4-azidobenzoyl)cystamine, for analysis of protein-nucleic acid interactions, has been synthesized. The reagent contains two photosensitive groups that can be activated sequentially. The azidoacridinyl moiety is sensitive to u.v. and visible light (lambda less than or equal to 450 nm), whereas the azidobenzoyl part needs higher-energy light (lambda less than or equal to 350 nm). Furthermore, the disulphide bridge connecting the two photoactive groups can be cleaved by reduction with mercaptans. The reagent is shown to induce cleavable cross-links between all five major histones and DNA in chromatin from Ehrlich ascites cells on activation with long-wavelength u.v. light (lambda greater than 300 nm) at an efficiency of approximately 3% of the added reagent.

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