Effects of 4-aminopyrazolo(3, 4-d)pyrimidine in combination with guanine on nucleic acid and protein synthesis by Ehrlich ascites cells in culture

1968 ◽  
Vol 50 (1) ◽  
pp. 73-80 ◽  
Author(s):  
D.O. Schachtschabel ◽  
D. Killander ◽  
A. Zetterberg ◽  
R.E. Mccarthy ◽  
G.E. Foley
Keyword(s):  
Protein Synthesis ◽  
Nucleic Acid ◽  
Cells In Culture ◽  
Ehrlich Ascites ◽  
Ehrlich Ascites Cells

scholarly journals Studies on the proteins from the seeds of Croton tiglium and of Jatropha curcas. Toxic properties and inhibition of protein synthesis in vitro

1976 ◽  
Vol 156 (1) ◽  
pp. 1-6 ◽  
Author(s):  
F Stirpe ◽  
A Pession-Brizzi ◽  
E Lorenzoni ◽  
P Strocchi ◽  
L Montanaro ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Jatropha Curcas ◽  
Crude Protein ◽  
Croton Tiglium ◽  
Ehrlich Ascites ◽  
Maximum Inhibition ◽  
Ehrlich Ascites Cells ◽  
Inhibition Of Protein Synthesis ◽  
Reticulocyte Lysate

1. Proteins extracted from the seeds of the Euphorbiaceae croton tiglium and Jatropha curcas were separated into three major peaks (I, II, and III) by Sephadex chromatography. 2. The crude protein from both seeds and peaks I and II from Croton and peak I from Jatropha were toxic to mice, to different extents. 3. The crude protein and peak I and peak II from both seeds, inhibited protein synthesis by a reticulocyte lysate; maximum inhibition was exerted by peak II from both seeds. None of these preparations affected protein synthesis in vitro by Ehrlich ascites cells.

scholarly journals Modeccin, the toxin of Adenia digitata. Purification, toxicity and inhibition of protein synthesis in vitro

1978 ◽  
Vol 174 (2) ◽  
pp. 491-496 ◽  
Author(s):  
A Gasperi-Campani ◽  
L Barbieri ◽  
E Lorenzoni ◽  
L Montanaro ◽  
S Sperti ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Affinity Chromatography ◽  
Ehrlich Ascites ◽  
Ehrlich Ascites Cells ◽  
Inhibition Of Protein Synthesis ◽  
Sepharose 4B ◽  
Rabbit Reticulocytes ◽  
In Cells

1. Modeccin, the toxin of Adenia digitata (Modecca digitata), was purified from the roots of this plant by affinity chromatography on Sepharose 4B. 2. This toxin is a protein with mol.wt. 57000, which on treatment with 2-mercaptoethanol can be dissociated into two subunits of mol.wts. 25000 and 32000. 3. Modeccin inhibits protein synthesis in vitro in a lysate of rabbit reticulocytes and in Ehrlich ascites cells; the effect on cells is decreased in the presence of lactose. 4. Dissociation of modeccin into subunits decreases the toxicity to animals and the inhibition of protein synthesis in cells, but enhances the inhibition of protein synthesis in the lysate system.

A factor reducing protein synthesis from Ehrlich ascites cells

1973 ◽  
Vol 9 (11-12) ◽  
pp. 819-824 ◽  
Author(s):  
D. Werner ◽  
G. Maier ◽  
R. Lommel
Keyword(s):  
Protein Synthesis ◽  
Ehrlich Ascites ◽  
Ehrlich Ascites Cells
Sign in / Sign up

Export Citation Format

Share Document