Microsurgical repair after crush-avulsion injury of the femoral vein in rats: Prevention of microvascular thrombosis with recombinant human tissue-type plasminogen activator (rt-PA)

Abstract The influence of the presence of fibrin microclots on the systemic fibrinogenolytic effects of intravenous (IV) recombinant human tissue- type plasminogen activator (rt-PA) was studied by injection of a homogenized fibrin suspension in the femoral vein or artery in rabbits. A linear correlation (P less than .001) was found between the extent of fibrinogen breakdown and the amount of fibrin (0 to 32 mg/kg) injected just prior to the IV infusion of rt-PA at a rate of 10 micrograms/kg/min for 60 minutes. This finding suggests that the systemic activation of the fibrinolytic system observed in some patients during infusion of rt-PA may be due, at least in part, to the presence of fibrin in the vascular bed. The effect of blood flow in the liver on the turnover of rt-PA was measured in rabbits after ligation of the hepatic artery and monitoring of the blood flow in the portal vein with a peristaltic pump. The half-life (t1/2) of rt-PA in plasma was inversely correlated with the logarithm of the rate of the liver blood flow. A doubling of the plasma t1/2 of rt-PA was observed after an eightfold reduction of the liver blood flow, suggesting that delayed clearance of rt-PA may occur in patients with severe cardiovascular failure and impaired liver blood flow.

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Microvascular repair following a modified crush-avulsion injury in a rat model: Effect of recombinant human tissue-type plasminogen activator on the patency rate

Microsurgery ◽

10.1002/(sici)1098-2752(2000)20:2<52::aid-micr2>3.0.co;2-g ◽

2000 ◽

Vol 20 (2) ◽

pp. 52-58 ◽

Cited By ~ 9

Author(s):

Efstathios G. Lykoudis ◽

Petros N. Panayotou ◽

Constantinos N. Stamatopoulos ◽

Konstantina B. Frangia ◽

Apostolos E. Papalois ◽

...

Keyword(s):

Plasminogen Activator ◽

Rat Model ◽

Human Tissue ◽

Patency Rate ◽

Tissue Type ◽

Tissue Type Plasminogen Activator ◽

Avulsion Injury ◽

Type Plasminogen Activator

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Prevention of Microvascular Thrombosis with Short-Term Infusion of Human Tissue-Type Plasminogen Activator

Plastic & Reconstructive Surgery ◽

10.1097/00006534-199607000-00019 ◽

1996 ◽

Vol 98 (1) ◽

pp. 118-128 ◽

Cited By ~ 22

Author(s):

Rod J. Rohrich ◽

John Handren ◽

Robert Kersh ◽

Charles A. Hergrueter ◽

James W. May

Keyword(s):

Plasminogen Activator ◽

Human Tissue ◽

Tissue Type ◽

Short Term ◽

Tissue Type Plasminogen Activator ◽

Microvascular Thrombosis ◽

Type Plasminogen Activator

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Influence of fibrin and liver blood flow on the turnover and the systemic fibrinogenolytic effects of recombinant human tissue-type plasminogen activator in rabbits

Blood ◽

10.1182/blood.v67.5.1493.bloodjournal6751493 ◽

1986 ◽

Vol 67 (5) ◽

pp. 1493-1497

Author(s):

H Bounameaux ◽

JM Stassen ◽

C Seghers ◽

D Collen

Keyword(s):

Blood Flow ◽

Plasminogen Activator ◽

Human Tissue ◽

Femoral Vein ◽

Liver Blood Flow ◽

Tissue Type ◽

Tissue Type Plasminogen Activator ◽

Cardiovascular Failure ◽

Type Plasminogen Activator ◽

Impaired Liver

The influence of the presence of fibrin microclots on the systemic fibrinogenolytic effects of intravenous (IV) recombinant human tissue- type plasminogen activator (rt-PA) was studied by injection of a homogenized fibrin suspension in the femoral vein or artery in rabbits. A linear correlation (P less than .001) was found between the extent of fibrinogen breakdown and the amount of fibrin (0 to 32 mg/kg) injected just prior to the IV infusion of rt-PA at a rate of 10 micrograms/kg/min for 60 minutes. This finding suggests that the systemic activation of the fibrinolytic system observed in some patients during infusion of rt-PA may be due, at least in part, to the presence of fibrin in the vascular bed. The effect of blood flow in the liver on the turnover of rt-PA was measured in rabbits after ligation of the hepatic artery and monitoring of the blood flow in the portal vein with a peristaltic pump. The half-life (t1/2) of rt-PA in plasma was inversely correlated with the logarithm of the rate of the liver blood flow. A doubling of the plasma t1/2 of rt-PA was observed after an eightfold reduction of the liver blood flow, suggesting that delayed clearance of rt-PA may occur in patients with severe cardiovascular failure and impaired liver blood flow.

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