STAT5 plays a critical role in regulating the 5′-flanking region of the porcine whey acidic protein gene in transgenic mice

2015 ◽  
Vol 82 (12) ◽  
pp. 957-966 ◽  
Author(s):  
Mi-ran Ji ◽  
Sang In Lee ◽  
Ye-Jin Jang ◽  
Mi-hyang Jeon ◽  
Jeom Sun Kim ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Protein Gene ◽  
Critical Role ◽  
Whey Acidic Protein ◽  
Acidic Protein ◽  
Flanking Region

Rabbit whey acidic protein gene upstream region controls high-level expression of bovine growth hormone in the mammary gland of transgenic mice

1995 ◽  
Vol 42 (3) ◽  
pp. 261-267 ◽  
Author(s):  
Dominique Thépot ◽  
Eve Devinoy ◽  
Marie-Louise Fontaine ◽  
Marie-Georges Stinnakre ◽  
Micheline Massoud ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Mammary Gland ◽  
Transgenic Mice ◽  
Protein Gene ◽  
Whey Acidic Protein ◽  
Acidic Protein ◽  
Upstream Region ◽  
Bovine Growth Hormone ◽  
High Level Expression ◽  
High Level

Position-independent and tissue-specific expression of porcine whey acidic protein gene from a bacterial artificial chromosome in transgenic mice

2002 ◽  
Vol 63 (2) ◽  
pp. 161-167 ◽  
Author(s):  
Sylvie Rival-Gervier ◽  
C�line Viglietta ◽  
Caroline Maeder ◽  
Joe Attal ◽  
Louis-Marie Houdebine
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Transgenic Mice ◽  
Protein Gene ◽  
Artificial Chromosome ◽  
Whey Acidic Protein ◽  
Acidic Protein ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression

Expression of Biologically Active Human Interferon Gamma in the Milk of Transgenic Mice Under the Control of the Murine Whey Acidic Protein Gene Promoter

2010 ◽  
Vol 49 (3-4) ◽  
pp. 251-257 ◽  
Author(s):  
Haydar Bagis ◽  
Digdem Aktoprakligil ◽  
Cagatay Gunes ◽  
Sezen Arat ◽  
Tolga Akkoc ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Interferon Gamma ◽  
Protein Gene ◽  
Gene Promoter ◽  
Whey Acidic Protein ◽  
Acidic Protein ◽  
Biologically Active ◽  
Human Interferon

Analysis of the efficiency of the rabbit whey acidic protein gene 5′ flanking region in controlling the expression of homologous and heterologous linked genes

2005 ◽  
Vol 72 (S1) ◽  
pp. 113-119 ◽  
Author(s):  
Eve Devinoy ◽  
Lluís Montoliu ◽  
Mária Baranyi ◽  
Dominique Thépot ◽  
László Hiripi ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Large Scale ◽  
Protein Gene ◽  
Regulatory Region ◽  
Whey Acidic Protein ◽  
Acidic Protein ◽  
Heterologous Proteins ◽  
Regulatory Regions ◽  
Expression Levels ◽  
Function Relationship

For 10 years, the regulatory regions of the mouse and rabbit whey acidic protein gene have been used to express heterologous proteins in the milk of transgenic mice, as well as to produce pharmaceutical proteins, on a large scale, in the milk of transgenic livestock. To date, a broad range of expression levels have been detected, and elucidation of the structure-function relationship in these regulatory regions might help to achieve high levels of expression, reproducibly. An extended 5′ regulatory region (17·6 kb v. 6·3 kb) of the rabbit whey acidic promoter resulted in an increased frequency of rabbit whey acidic protein expression in transgenic mice. However, the expression levels were low compared with the high expression levels achieved in both transgenic mice and rabbits using the heterologous κ-casein in the 6·3 kb rabbit whey acidic protein 5′ regulatory region. These results underline the importance of the 3’ downstream regulatory regions, which still need to be better characterized in the whey acidic protein gene.

scholarly journals Comparison of the Regulation of the Whey Acidic Protein Gene with that of a Hybrid Gene Containing the Whey Acidic Protein Gene Promoter in Transgenic Mice

1988 ◽  
Vol 2 (11) ◽  
pp. 1027-1032 ◽  
Author(s):  
Christoph W. Pittius ◽  
Lakshmanan Sankaran ◽  
Yale J. Topper ◽  
Lothar Hennighausen
Keyword(s):  
Transgenic Mice ◽  
Protein Gene ◽  
Gene Promoter ◽  
Whey Acidic Protein ◽  
Acidic Protein ◽  
Hybrid Gene

A Neural-Specific Hypomethylated Domain in the 5' Flanking Region of the Glial Fibrillary Acidic Protein Gene

1997 ◽  
Vol 19 (5) ◽  
pp. 446-456 ◽  
Author(s):  
D.F. Condorelli ◽  
P. Dell'Albani ◽  
S.G. Conticello ◽  
V. Barresi ◽  
V.G. Nicoletti ◽  
...  
Keyword(s):  
Glial Fibrillary Acidic Protein ◽  
Protein Gene ◽  
Acidic Protein ◽  
Flanking Region

scholarly journals Complete sequence of the rabbit whey acidic protein gene

1990 ◽  
Vol 18 (12) ◽  
pp. 3641-3641 ◽  
Author(s):  
D. Thepot ◽  
E. Devinoy ◽  
M.L. Fontaine ◽  
C. Hubert ◽  
L.M. Houdebine
Keyword(s):  
Protein Gene ◽  
Complete Sequence ◽  
Whey Acidic Protein ◽  
Acidic Protein

scholarly journals Nuclear factor I and mammary gland factor (STAT5) play a critical role in regulating rat whey acidic protein gene expression in transgenic mice.

1995 ◽  
Vol 15 (4) ◽  
pp. 2063-2070 ◽  
Author(s):  
S Li ◽  
J M Rosen
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Transgenic Mice ◽  
Binding Sites ◽  
Transgene Expression ◽  
Protein Gene ◽  
Whey Acidic Protein ◽  
Nuclear Factor ◽  
Factor I ◽  
Nuclear Factor I

The rat whey acidic protein (WAP) gene contains a mammary gland-specific and hormonally regulated DNase I-hypersensitive site 830 to 720 bp 5' to the site of transcription initiation. We have reported previously that nuclear factor I (NFI) binding at a palindromic site and binding at a half-site are the major DNA-protein interactions detected within this tissue-specific nuclease-hypersensitive region. We now show that point mutations introduced into these NFI-binding sites dramatically affect WAP gene expression in transgenic mice. Transgene expression was totally abrogated when the palindromic NFI site or both binding sites were mutated, suggesting that NFI is a key regulator of WAP gene expression. In addition, a recognition site for mammary gland factor (STAT5), which mediates prolactin induction of milk protein gene expression, was also identified immediately proximal to the NFI-binding sites. Mutation of this site reduced transgene expression by approximately 90% per gene copy, but did not alter tissue specificity. These results suggest that regulation of WAP gene expression is determined by the cooperative interactions among several enhancers that constitute a composite response element.

Ruminants genome no longer contains Whey Acidic Protein gene but only a pseudogene

Gene ◽  
2006 ◽  
Vol 370 ◽  
pp. 104-112 ◽  
Author(s):  
Siham Hajjoubi ◽  
Sylvie Rival-Gervier ◽  
Hélène Hayes ◽  
Sandrine Floriot ◽  
André Eggen ◽  
...  
Keyword(s):  
Protein Gene ◽  
Whey Acidic Protein ◽  
Acidic Protein
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