Synaptic vesicle cycling at type-identified diaphragm neuromuscular junctions

2004 ◽  
Vol 30 (6) ◽  
pp. 774-783 ◽  
Author(s):  
Carlos B. Mantilla ◽  
Katharine L. Rowley ◽  
Mohamed A. Fahim ◽  
Wen-Zhi Zhan ◽  
Gary C. Sieck
Keyword(s):  
Synaptic Vesicle ◽  
Neuromuscular Junctions ◽  
Vesicle Cycling

scholarly journals Synaptic vesicle cycling is not impaired in a glutamatergic and a cholinergic synapse that exhibit deficits in acidification and filling

2012 ◽  
Vol 48 (1) ◽  
pp. 155-161
Author(s):  
Bento João Abreu ◽  
Luciana Ferreira Leite ◽  
Débora Lopes Oliveira ◽  
Ernani Amaral
Keyword(s):  
Synaptic Vesicle ◽  
Neuromuscular Junctions ◽  
Vesicle Trafficking ◽  
Experimental Models ◽  
Frog Neuromuscular Junction ◽  
Experimental Conditions ◽  
Pharmacological Agents ◽  
Vesicle Cycling ◽  
Functional Differences ◽  
Styryl Dye

The purpose of the present work was to investigate synaptic vesicle trafficking when vesicles exhibit alterations in filling and acidification in two different synapses: a cholinergic frog neuromuscular junction and a glutamatergic ribbon-type nerve terminal in the retina. These synapses display remarkable structural and functional differences, and the mechanisms regulating synaptic vesicle cycling might also differ between them. The lipophilic styryl dye FM1-43 was used to monitor vesicle trafficking. Both preparations were exposed to pharmacological agents that collapse ΔpH (NH4Cl and methylamine) or the whole ΔµH+ (bafilomycin), a necessary situation to provide the driving force for neurotransmitter accumulation into synaptic vesicles. The results showed that FM1-43 loading and unloading in neuromuscular junctions did not differ statistically between control and experimental conditions (P > 0.05). Also, FM1-43 labeling in bipolar cell terminals proved highly similar under all conditions tested. Despite remarkable differences in both experimental models, the present findings show that acidification and filling are not required for normal vesicle trafficking in either synapse.

24S-Hydroxycholesterol enhances synaptic vesicle cycling in the mouse neuromuscular junction: Implication of glutamate NMDA receptors and nitric oxide

2017 ◽  
Vol 117 ◽  
pp. 61-73 ◽  
Author(s):  
M.R. Kasimov ◽  
M.R. Fatkhrakhmanova ◽  
K.A. Mukhutdinova ◽  
A.M. Petrov
Keyword(s):  
Nitric Oxide ◽  
Neuromuscular Junction ◽  
Nmda Receptors ◽  
Synaptic Vesicle ◽  
Vesicle Cycling ◽  
Glutamate Nmda Receptors

scholarly journals Impaired Synaptic Vesicle Release and Immaturity of Neuromuscular Junctions in Spinal Muscular Atrophy Mice

2009 ◽  
Vol 29 (3) ◽  
pp. 842-851 ◽  
Author(s):  
L. Kong ◽  
X. Wang ◽  
D. W. Choe ◽  
M. Polley ◽  
B. G. Burnett ◽  
...  
Keyword(s):  
Spinal Muscular Atrophy ◽  
Synaptic Vesicle ◽  
Neuromuscular Junctions ◽  
Muscular Atrophy ◽  
Vesicle Release ◽  
Synaptic Vesicle Release

scholarly journals Identification of a Novel Process Limiting the Rate of Synaptic Vesicle Cycling at Hippocampal Synapses

Neuron ◽  
1999 ◽  
Vol 24 (4) ◽  
pp. 1017-1028 ◽  
Author(s):  
Charles F. Stevens ◽  
John F. Wesseling
Keyword(s):  
Synaptic Vesicle ◽  
Vesicle Cycling ◽  
Hippocampal Synapses

Unique biochemical and behavioral alterations indrosophilashibirets1mutants imply a conformational state affecting dynamin subcellular distribution and synaptic vesicle cycling

2002 ◽  
Vol 53 (3) ◽  
pp. 319-329 ◽  
Author(s):  
Mai-Lei Chen ◽  
David Green ◽  
Lei Liu ◽  
Yung Carmen Lam ◽  
Leona Mukai ◽  
...  
Keyword(s):  
Synaptic Vesicle ◽  
Subcellular Distribution ◽  
Conformational State ◽  
Behavioral Alterations ◽  
Vesicle Cycling

Fixation effects on synaptic vesicle density in neuromuscular junctions of young and old mice

1987 ◽  
Vol 8 (1) ◽  
pp. 71-75 ◽  
Author(s):  
Mohamed A. Fahim ◽  
Norman Robbins ◽  
Robert Price
Keyword(s):  
Synaptic Vesicle ◽  
Neuromuscular Junctions ◽  
Old Mice

Intermediates in synaptic vesicle recycling revealed by optical imaging of Drosophila neuromuscular junctions

Neuron ◽  
1994 ◽  
Vol 13 (2) ◽  
pp. 363-375 ◽  
Author(s):  
Mani Ramaswami ◽  
K.S. Krishnan ◽  
Regis B. Kelly
Keyword(s):  
Optical Imaging ◽  
Synaptic Vesicle ◽  
Neuromuscular Junctions ◽  
Synaptic Vesicle Recycling ◽  
Vesicle Recycling

scholarly journals Identification of a human synaptotagmin-1 mutation that perturbs synaptic vesicle cycling

2015 ◽  
Author(s):  
Kate Baker ◽  
Sarah L. Gordon ◽  
Detelina Grozeva ◽  
Margriet van Kogelenberg ◽  
Nicola Y. Roberts ◽  
...  
Keyword(s):  
Synaptic Vesicle ◽  
Vesicle Cycling ◽  
Synaptotagmin 1

scholarly journals Hypothesis Relating the Structure, Biochemistry and Function of Active Zone Material Macromolecules at a Neuromuscular Junction

2022 ◽  
Vol 13 ◽  
Author(s):  
Joseph A. Szule
Keyword(s):  
Membrane Fusion ◽  
Synaptic Vesicle ◽  
Active Zone ◽  
Structural Integrity ◽  
Neuromuscular Junctions ◽  
Vesicle Trafficking ◽  
Material Structure ◽  
Neurotransmitter Secretion ◽  
Active Zones ◽  
And Function

This report integrates knowledge of in situ macromolecular structures and synaptic protein biochemistry to propose a unified hypothesis for the regulation of certain vesicle trafficking events (i.e., docking, priming, Ca2+-triggering, and membrane fusion) that lead to neurotransmitter secretion from specialized “active zones” of presynaptic axon terminals. Advancements in electron tomography, to image tissue sections in 3D at nanometer scale resolution, have led to structural characterizations of a network of different classes of macromolecules at the active zone, called “Active Zone Material’. At frog neuromuscular junctions, the classes of Active Zone Material macromolecules “top-masts”, “booms”, “spars”, “ribs” and “pins” direct synaptic vesicle docking while “pins”, “ribs” and “pegs” regulate priming to influence Ca2+-triggering and membrane fusion. Other classes, “beams”, “steps”, “masts”, and “synaptic vesicle luminal filaments’ likely help organize and maintain the structural integrity of active zones. Extensive studies on the biochemistry that regulates secretion have led to comprehensive characterizations of the many conserved proteins universally involved in these trafficking events. Here, a hypothesis including a partial proteomic atlas of Active Zone Material is presented which considers the common roles, binding partners, physical features/structure, and relative positioning in the axon terminal of both the proteins and classes of macromolecules involved in the vesicle trafficking events. The hypothesis designates voltage-gated Ca2+ channels and Ca2+-gated K+ channels to ribs and pegs that are connected to macromolecules that span the presynaptic membrane at the active zone. SNARE proteins (Syntaxin, SNAP25, and Synaptobrevin), SNARE-interacting proteins Synaptotagmin, Munc13, Munc18, Complexin, and NSF are designated to ribs and/or pins. Rab3A and Rabphillin-3A are designated to top-masts and/or booms and/or spars. RIM, Bassoon, and Piccolo are designated to beams, steps, masts, ribs, spars, booms, and top-masts. Spectrin is designated to beams. Lastly, the luminal portions of SV2 are thought to form the bulk of the observed synaptic vesicle luminal filaments. The goal here is to help direct future studies that aim to bridge Active Zone Material structure, biochemistry, and function to ultimately determine how it regulates the trafficking events in vivo that lead to neurotransmitter secretion.

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