Analysis of microsatellite alterations in gastric carcinoma using the crypt isolation technique

2004 ◽  
Vol 204 (2) ◽  
pp. 200-207 ◽  
Author(s):  
Yu-Fei Jiao ◽  
Tamotsu Sugai ◽  
Wataru Habano ◽  
Masamichi Suzuki ◽  
Akinori Takagane ◽  
...  
Keyword(s):  
Gastric Carcinoma ◽  
Isolation Technique ◽  
Crypt Isolation

scholarly journals Genetic alterations in DNA diploid, aneuploid and multiploid colorectal carcinomas identified by the crypt isolation technique

2000 ◽  
Vol 88 (4) ◽  
pp. 614-619 ◽  
Author(s):  
Tamotsu Sugai ◽  
Wataru Habano ◽  
Shin-ichi Nakamura ◽  
Hajime Sato ◽  
Noriyuki Uesugi ◽  
...  
Keyword(s):  
Genetic Alterations ◽  
Colorectal Carcinomas ◽  
Isolation Technique ◽  
Crypt Isolation

A Unique Method for Mutation Analysis of Tumor Suppressor Genes in Colorectal Carcinomas Using a Crypt Isolation Technique

2000 ◽  
Vol 124 (3) ◽  
pp. 382-386 ◽  
Author(s):  
Tamotsu Sugai ◽  
Wataru Habano ◽  
Shin-ichi Nakamura ◽  
Noriyuki Uesugi ◽  
Shunichi Sasou ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Genetic Analysis ◽  
Tumor Suppressor Genes ◽  
Single Strand Conformation Polymorphism ◽  
Single Strand ◽  
Colorectal Carcinomas ◽  
Suppressor Genes ◽  
Isolation Technique ◽  
Crypt Isolation ◽  
Conformation Polymorphism

Abstract Background.—Contamination of nontumor tissue makes genetic analysis difficult. For this reason, it is important to obtain pure tumor tissue to ensure accurate genetic analysis. Objective.—To accurately assess the incidence of mutation of tumor suppressor genes (p53: exon 5–8; APC: mutated cluster region; NF-2 gene: all exons) in 45 colorectal carcinomas. Methods.—We developed an application of the polymerase chain reaction–single-strand conformation polymorphism and DNA sequence by coupling them with crypt isolation. Results.—Mutations of p53 and APC genes were found in 24 and 22 of 45 colorectal carcinomas, respectively. No mutation of the NF-2 gene was observed in this cancer. Single-strand conformation polymorphism using a crypt isolation technique showed a clear migrating band and no false-positive data. Conclusions.—The crypt isolation technique is a useful method for accurately analyzing genetic alterations. Furthermore, our proposed method confirmed the morphological findings obtained before the genetic analysis.

Application of the crypt-isolation technique to flow-cytometric analysis of DNA content in colorectal neoplasms

1994 ◽  
Vol 106 (1) ◽  
pp. 100-107 ◽  
Author(s):  
Shin-Ichi Nakamura ◽  
Junko Goto ◽  
Masahiko Kitayama ◽  
Isamu Kino
Keyword(s):  
Colorectal Neoplasms ◽  
Dna Content ◽  
Flow Cytometric Analysis ◽  
Isolation Technique ◽  
Flow Cytometric ◽  
Crypt Isolation

scholarly journals Clinicopathological significance of loss of heterozygosity in intestinal- and solid-type gastric carcinomas: a comprehensive study using the crypt isolation technique

2006 ◽  
Vol 19 (4) ◽  
pp. 548-555 ◽  
Author(s):  
Yu-Fei Jiao ◽  
Tamotsu Sugai ◽  
Wataru Habano ◽  
Noriyuki Uesugi ◽  
Akinori Takagane ◽  
...  
Keyword(s):  
Loss Of Heterozygosity ◽  
Isolation Technique ◽  
Gastric Carcinomas ◽  
Clinicopathological Significance ◽  
Crypt Isolation ◽  
Solid Type ◽  
Comprehensive Study

An isolation technique to prevent the spread of tumor cells during radical gastrectomy for gastric carcinoma located on the anterior wall of the gastric antrum

2013 ◽  
Vol 39 (10) ◽  
pp. 1136-1143 ◽  
Author(s):  
J. Wang ◽  
X. Mao ◽  
F. Guo ◽  
X. Zhang ◽  
M. Guan ◽  
...  
Keyword(s):  
Gastric Carcinoma ◽  
Tumor Cells ◽  
Anterior Wall ◽  
Gastric Antrum ◽  
Radical Gastrectomy ◽  
Isolation Technique

Evolution of DNA ploidy state and DNA index in colorectal adenomas and carcinomas using the crypt isolation technique: New hypothesis in colorectal tumorigenesis

2003 ◽  
Vol 53 (3) ◽  
pp. 154-162 ◽  
Author(s):  
Tamotsu Sugai ◽  
Noriyuki Uesugi ◽  
Shin-ichi Nakamura ◽  
Wataru Habano ◽  
Yu-Fei Jiao ◽  
...  
Keyword(s):  
Dna Ploidy ◽  
Colorectal Adenomas ◽  
Dna Index ◽  
Colorectal Tumorigenesis ◽  
Isolation Technique ◽  
Crypt Isolation ◽  
New Hypothesis

scholarly journals Analysis of Ki-ras gene mutations associated with DNA diploid, aneuploid, and multiploid colorectal carcinomas using a crypt isolation technique

Cytometry ◽  
2001 ◽  
Vol 46 (6) ◽  
pp. 345-350 ◽  
Author(s):  
Tamotsu Sugai ◽  
Wataru Habano ◽  
Shin-ichi Nakamura ◽  
Yu-Fei Jiao ◽  
Taro Higuchi ◽  
...  
Keyword(s):  
Gene Mutations ◽  
Colorectal Carcinomas ◽  
Ras Gene ◽  
Isolation Technique ◽  
Crypt Isolation

Application of the crypt isolation technique to the assessment of genetic alterations of colorectal carcinomas

2002 ◽  
Vol 52 (10) ◽  
pp. 628-635 ◽  
Author(s):  
Hiroshi Takahashi ◽  
Tamotsu Sugai ◽  
Wataru Habano ◽  
Shin-ichi Nakamura
Keyword(s):  
Genetic Alterations ◽  
Colorectal Carcinomas ◽  
Isolation Technique ◽  
Crypt Isolation

Gastric carcinoma with prominent neutrophil infiltration<

2000 ◽  
Vol 37 (3) ◽  
pp. 289-290 ◽  
Author(s):  
A J Rice ◽  
A P Griffiths ◽  
I G Martin ◽  
M F Dixon
Keyword(s):  
Gastric Carcinoma ◽  
Neutrophil Infiltration
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