Relationship between the presence of oncogenic HPV DNA assessed by polymerase chain reaction and Ki-67 immunoquantitative features in cervical intraepithelial neoplasia

2001 ◽  
Vol 195 (5) ◽  
pp. 557-562 ◽  
Author(s):  
Arnold-Jan Kruse ◽  
Jan P. A. Baak ◽  
Peter C. de Bruin ◽  
Frank R. W. van de Goot ◽  
Nicolette Kurten
Keyword(s):  
Polymerase Chain Reaction ◽  
Cervical Intraepithelial Neoplasia ◽  
Intraepithelial Neoplasia ◽  
Chain Reaction ◽  
Ki 67 ◽  
Hpv Dna ◽  
Polymerase Chain ◽  
Oncogenic Hpv

The Utility of p16Ink4a in Discriminating Between Cervical Intraepithelial Neoplasia 1 and Nonneoplastic Equivocal Lesions of the Cervix

2008 ◽  
Vol 132 (5) ◽  
pp. 795-799
Author(s):  
Rachel Redman ◽  
Irina Rufforny ◽  
Chen Liu ◽  
Edward J. Wilkinson ◽  
Nicole A. Massoll
Keyword(s):  
Polymerase Chain Reaction ◽  
High Risk ◽  
Cervical Intraepithelial Neoplasia ◽  
Real Time ◽  
Intraepithelial Neoplasia ◽  
Cervical Lesions ◽  
Chain Reaction ◽  
Cervical Biopsy ◽  
Polymerase Chain ◽  
High Risk Hpv

Abstract Context.—The protein p16Ink4a is overexpressed in cervical lesions associated with high-risk human papillomavirus (HPV) subtypes 16 and 18, but not in low-risk HPV subtypes 6 and 11 or non–HPV-associated cervical lesions. Objective.—To determine whether p16Ink4a expression in equivocal cervical lesions helps distinguish atypical non-HPV changes from HPV-related changes. Design.—One hundred ninety-one cervical lesions, including 81 cervical intraepithelial neoplasia 1, 52 squamous metaplasia, 33 cellular features suggestive of HPV-related change, 9 reserve cell hyperplasia, 4 microglandular hyperplasia, and 12 inflammatory cervicitis, were randomly selected from archival cervical biopsy specimens. All 191 samples were studied with p16Ink4a (JC8 monoclonal antibody). Reactivity for p16Ink4a was scored on a 3-tier system as follows: negative, 0% to 5% cells reactive; focal/scattered positive, greater than 5% and less than or equal to 80% cells reactive; diffuse positive, greater than 80% cells reactive. Reactivity was based on normal/reactive cervical specimens where anti-p16 antibody was negative/weakly expressed in non–cervical epithelial cells. Cervical intraepithelial neoplasia 1 lesions not reactive for p16Ink4a were investigated for the presence of high-risk HPV by real-time polymerase chain reaction. Results.—No p16Ink4a reactivity was detected in the cervical lesions associated with atypical non-HPV change. Eleven of the cervical intraepithelial neoplasia 1 lesions showed focal/scattered reactivity expression for p16Ink4a, and 19 of the CIN 1 lesions had diffuse reactivity. Fifty of 51 of the CIN 1 lesions negative for p16Ink4a were real-time polymerase chain reaction negative for the presence of high-risk HPV; 1 was real-time polymerase chain reaction positive for high-risk HPV. Conclusions.—The data support the routine use of p16Ink4a immunohistochemical evaluation of cervical biopsy specimens for better discrimination of non–HPV-associated lesions from HPV-related lesions.

The Impact of the Distribution of Human Papillomavirus Types and Associated High-Risk Lesions in a Colposcopy Population for Monitoring Vaccine Efficacy

2008 ◽  
Vol 132 (1) ◽  
pp. 54-60
Author(s):  
Nick A. Antonishyn ◽  
Greg B. Horsman ◽  
Rod A. Kelln ◽  
Jasdeep Saggar ◽  
Alberto Severini
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papillomavirus ◽  
Cervical Intraepithelial Neoplasia ◽  
Real Time ◽  
Intraepithelial Neoplasia ◽  
Chain Reaction ◽  
Hpv 16 ◽  
Hpv Types ◽  
Polymerase Chain ◽  
Hpv 18

Abstract Context.—Impact studies of the new human papillomavirus (HPV) vaccines will be biased unless local baseline distribution studies are conducted. Vaccine cross protection for other important oncogenic HPV types and the emergence of potential genotype replacements require the knowledge of the prevaccine epidemiology of HPV. Objective.—To determine the prevaccine distribution of HPV types in Saskatchewan, using a subpopulation of women referred to a colposcopy clinic. Design.—One thousand three hundred fifty-five specimens obtained during colposcopic examination were typed for HPV using L1 or E1 gene polymerase chain reaction and direct sequencing. HPV-16 and HPV-31 infections were confirmed with real-time E6 polymerase chain reaction. Indeterminate samples were analyzed using Luminex technology. Correlations of the HPV type and histology were examined for statistical significance. Results.—The most commonly identified genotype in patients with cervical intraepithelial neoplasia grade 2 or worse was HPV-16 (46.7%) followed by HPV-31 (14.7%) and then HPV-18 (3.9%). Fifteen of 330 specimens that were positive for HPV-16 or HPV-31 were further resolved to be mixed HPV-16/HPV-31 infections by real-time polymerase chain reaction. The risk of cervical intraepithelial neoplasia associated with HPV-18 infection (0.4–1.7) is substantially lower than with either HPV-16 (3.6–11.0) or HPV-31 (1.8–12.6). Conclusions.—HPV-31 is contributing significantly to the proportion of women with cervical intraepithelial neoplasia in our population and shows a higher prevalence than HPV-18 in high-grade lesions. The clinical significance of HPV-31 may be underestimated and its continued significance will depend on the level of cross protection offered by the new vaccines.

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