Demonstration of spontaneously dividing male fetal cells in maternal blood by negative magnetic cell sorting and fish

1995 ◽  
Vol 15 (6) ◽  
pp. 573-578 ◽  
Author(s):  
Yun-Ling Zheng ◽  
Sabrina D. Craigo ◽  
Cathy M. Price ◽  
Diana W. Bianchi
Keyword(s):  
Cell Sorting ◽  
Maternal Blood ◽  
Magnetic Cell Sorting ◽  
Fetal Cells

Enrichment of fetal cells from maternal blood by high gradient magnetic cell sorting (double MACS) for PCR-based genetic analysis

1994 ◽  
Vol 14 (12) ◽  
pp. 1129-1140 ◽  
Author(s):  
J. Büsch ◽  
P. Huber ◽  
E. Pflüger ◽  
St. Miltenyi ◽  
J. Holtz ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Cell Sorting ◽  
Maternal Blood ◽  
Magnetic Cell Sorting ◽  
High Gradient ◽  
Fetal Cells

Magnetic cell sorting and the transferrin receptor as potential means of prenatal diagnosis from maternal blood

1992 ◽  
Vol 166 (5) ◽  
pp. 1350-1355 ◽  
Author(s):  
Dorothee Gänshirt-Ahlert ◽  
Monika Burschyk ◽  
Henk S.P. Garritsen ◽  
Lisa Helmer ◽  
Peter Miny ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
Cell Sorting ◽  
Transferrin Receptor ◽  
Maternal Blood ◽  
Magnetic Cell Sorting

Evaluation of a Soybean Lectin–based Method for the Enrichment of Erythroblasts

2005 ◽  
Vol 53 (3) ◽  
pp. 329-330 ◽  
Author(s):  
Tatiana Babochkina ◽  
Susanne Mergenthaler ◽  
Olaf Lapaire ◽  
Vivian Kiefer ◽  
Hirofumi Yura ◽  
...  
Keyword(s):  
Comparative Study ◽  
Pregnant Women ◽  
Cell Sorting ◽  
Maternal Blood ◽  
Soybean Agglutinin ◽  
Magnetic Cell Sorting ◽  
Blood Samples ◽  
Soybean Lectin

We performed a comparative study of the enrichment of erythroblasts by a soybean agglutinin galactose-specific lectin method and a standardized magnetic cell-sorting (MACS) protocol. Blood samples, obtained from 11 pregnant women at between 11 and 40 weeks of gestation, were split and examined by each method in parallel. The number of erythroblasts recovered by the lectin method was approximately eightfold higher than the number obtained by MACS. Our data suggest that the lectin-based method may provide a better approach for the enrichment of rare fetal erythroblasts from maternal blood.

scholarly journals Prenatal diagnosis from maternal blood: simultaneous immunophenotyping and FISH of fetal nucleated erythrocytes isolated by negative magnetic cell sorting.

1993 ◽  
Vol 30 (12) ◽  
pp. 1051-1056 ◽  
Author(s):  
Y L Zheng ◽  
N P Carter ◽  
C M Price ◽  
S M Colman ◽  
P J Milton ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
Cell Sorting ◽  
Maternal Blood ◽  
Magnetic Cell Sorting

Detection of Fetal Trisomies 21 and 18 From Maternal Blood Using Triple Gradient and Magnetic Cell Sorting

1993 ◽  
Vol 30 (2-3) ◽  
pp. 194-201 ◽  
Author(s):  
DOROTHEE GÄNSHIRT-AHLERT ◽  
REGINA BÖRJESSON-STOLL ◽  
MONIKA BURSCHYK ◽  
ANGELIKA DOHR ◽  
HENK S.P. GARRITSEN ◽  
...  
Keyword(s):  
Cell Sorting ◽  
Maternal Blood ◽  
Magnetic Cell Sorting

Enrichment of fetal cells from maternal blood by magnetic activated cell sorting (MACS) with fetal cell specific antibodies: One-step versus two-step MACS

2002 ◽  
Vol 42 (2) ◽  
pp. 120-124 ◽  
Author(s):  
Xaio Xi Zhao ◽  
Yasuhiko Ozaki ◽  
Nobuhiro Suzumori ◽  
Tsuyoshi Sato ◽  
Kaoru Suzumori
Keyword(s):  
Cell Sorting ◽  
Maternal Blood ◽  
Fetal Cell ◽  
Specific Antibodies ◽  
Fetal Cells ◽  
One Step ◽  
Magnetic Activated Cell Sorting

scholarly journals Development of a Specific Monoclonal Antibody to Detect Male Cells Expressing the RPS4Y1 Protein

2021 ◽  
Vol 22 (4) ◽  
pp. 2001
Author(s):  
Silvia Spena ◽  
Chiara Cordiglieri ◽  
Isabella Garagiola ◽  
Flora Peyvandi
Keyword(s):  
Monoclonal Antibody ◽  
Prenatal Diagnosis ◽  
Maternal Blood ◽  
Limiting Factors ◽  
Inherited Disease ◽  
Specific Monoclonal Antibody ◽  
Native Form ◽  
Fetal Cells ◽  
Non Invasive ◽  
Reliable Isolation

Hemophilia is an X-linked recessive bleeding disorder. In pregnant women carrier of hemophilia, the fetal sex can be determined by non-invasive analysis of fetal DNA circulating in the maternal blood. However, in case of a male fetus, conventional invasive procedures are required for the diagnosis of hemophilia. Fetal cells, circulating in the maternal bloodstream, are an ideal target for a safe non-invasive prenatal diagnosis. Nevertheless, the small number of cells and the lack of specific fetal markers have been the most limiting factors for their isolation. We aimed to develop monoclonal antibodies (mAbs) against the ribosomal protein RPS4Y1 expressed in male cells. By Western blotting, immunoprecipitation and immunofluorescence analyses performed on cell lysates from male human hepatoma (HepG2) and female human embryonic kidney (HEK293) we developed and characterized a specific monoclonal antibody against the native form of the male RPS4Y1 protein that can distinguish male from female cells. The availability of the RPS4Y1-targeting monoclonal antibody should facilitate the development of novel methods for the reliable isolation of male fetal cells from the maternal blood and their future use for non-invasive prenatal diagnosis of X-linked inherited disease such as hemophilia.

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