Resolution and characterisation of multiple isoforms of bovine κ-casein by 2-DE following a reversible cysteine-tagging enrichment strategy

PROTEOMICS ◽  
2006 ◽  
Vol 6 (10) ◽  
pp. 3087-3095 ◽  
Author(s):  
John W. Holland ◽  
Hilton C. Deeth ◽  
Paul F. Alewood
Keyword(s):  
Enrichment Strategy

Abstract 391: Molecular-based enrichment strategy for Nectin-4 targeted Bicycle toxin conjugate BT8009

2021 ◽  
Author(s):  
Tara Gelb ◽  
Sean Santos ◽  
Amy Dickson ◽  
Carly Campbell ◽  
Mike Rigby ◽  
...  
Keyword(s):  
Enrichment Strategy

Strategy for the enrichment of protein biomarkers from diverse bacterial select agents

2021 ◽  
Vol 28 ◽  
Author(s):  
Sasikumar Sabna ◽  
Dev Vrat Kamboj ◽  
Ravi Bhushan Kumar ◽  
Prabhakar Babele ◽  
Sakshi Rajoria ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Species Identification ◽  
Immune Serum ◽  
Tandem Ms ◽  
Environmental Matrices ◽  
Bacterial Proteins ◽  
Conserved Regions ◽  
Groel Protein ◽  
Ms Analysis ◽  
Enrichment Strategy

Background: Some pathogenic bacteria can be potentially used for nefarious applications in the event of bioterrorism or biowarfare. Accurate identification of biological agent from clinical and diverse environmental matrices is of paramount importance for implementation of medical countermeasures and biothreat mitigation. Objective: A novel methodology is reported here for the development of a novel enrichment strategy for the generally conserved abundant bacterial proteins for an accurate downstream species identification using tandem MS analysis in biothreat scenario. Methods: Conserved regions in the common bacterial protein markers were analyzed using bioinformatic tools and stitched for a possible generic immuno-capture for an intended downstream MS/MS analysis. Phylogenetic analysis of selected proteins was carried out and synthetic constructs were generated for the expression of conserved stitched regions of 60 kDa chaperonin GroEL. Hyper-immune serum was raised against recombinant synthetic GroEL protein. Results: The conserved regions of common bacterial proteins were stitched for a possible generic immuno-capture and subsequent specific identification by tandem MS using variable regions of the molecule. Phylogenetic analysis of selected proteins was carried out and synthetic constructs were generated for the expression of conserved stitched regions of GroEL. In a proof-of-concept study, hyper-immune serum raised against recombinant synthetic GroEL protein exhibited reactivity with ~60 KDa proteins from the cell lysates of three bacterial species tested. Conclusion: The envisaged methodology can lead to the development of a novel enrichment strategy for the abundant bacterial proteins from complex environmental matrices for the downstream species identification with increased sensitivity and substantially reduce the time-to-result.

Development of a Sustainable Enrichment Strategy for Quantification of Mercury Ions in Complex Samples at the Sub-Parts per Billion Level

2014 ◽  
Vol 53 (38) ◽  
pp. 14565-14570 ◽  
Author(s):  
Matthew P. Tracey ◽  
Kazunori Koide
Keyword(s):  
Mercury Ions ◽  
Complex Samples ◽  
Enrichment Strategy

scholarly journals Sphere-Forming Capacity as an Enrichment Strategy for Epithelial-Like Stem Cells from Equine Skin

2014 ◽  
Vol 34 (4) ◽  
pp. 1291-1303 ◽  
Author(s):  
Bizunesh M. Borena ◽  
Evelyne Meyer ◽  
Koen Chiers ◽  
Ann Martens ◽  
Kristel Demeyere ◽  
...  
Keyword(s):  
Stem Cells ◽  
Enrichment Strategy

Poisonous India or the Importance of a Semantic and Multilingual Enrichment Strategy

2012 ◽  
pp. 252-263 ◽  
Author(s):  
Marlies Olensky ◽  
Juliane Stiller ◽  
Evelyn Dröge
Keyword(s):  
Enrichment Strategy

What Makes Adult Education Compelling? Diversity as Enrichment Strategy

2012 ◽  
pp. 129-143
Author(s):  
Joan Marques
Keyword(s):  
Adult Education ◽  
Enrichment Strategy

Impact of the biomarker enrichment strategy in drug development

2020 ◽  
Vol 20 (6) ◽  
pp. 611-618 ◽  
Author(s):  
Aline Bobato Lara Gongora ◽  
Leandro Jonata Carvalho Oliveira ◽  
Denis Leonardo Jardim
Keyword(s):  
Drug Development ◽  
Enrichment Strategy
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