Mass spectrometric characterization of recombinant rat 5-hydroxytryptamine receptor 1A (5-HT1A R) expressed in tsA201 human embryonic kidney cells

PROTEOMICS ◽  
2012 ◽  
Vol 12 (22) ◽  
pp. 3338-3342 ◽  
Author(s):  
Seok Heo ◽  
Jae-Won Yang ◽  
Marie L. Huber ◽  
Melanie Planyavsky ◽  
Keiryn L. Bennett ◽  
...  
Keyword(s):  
Mass Spectrometric ◽  
Kidney Cells ◽  
Human Embryonic Kidney ◽  
Human Embryonic Kidney Cells

Characterization of porcine epidemic diarrhea virus infectivity in human embryonic kidney cells

2017 ◽  
Vol 162 (8) ◽  
pp. 2415-2419 ◽  
Author(s):  
Jian Zhang ◽  
Longjun Guo ◽  
Yunfei Xu ◽  
Lijun Yang ◽  
Hongyan Shi ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Virus Infectivity ◽  
Kidney Cells ◽  
Human Embryonic Kidney ◽  
Human Embryonic Kidney Cells ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

Characterization of JC papovavirus adapted to growth in human embryonic kidney cells.

1980 ◽  
Vol 35 (2) ◽  
pp. 498-504 ◽  
Author(s):  
T Miyamura ◽  
K Yoshiike ◽  
K K Takemoto
Keyword(s):  
Kidney Cells ◽  
Human Embryonic Kidney ◽  
Human Embryonic Kidney Cells

scholarly journals Two New Antiprotozoal Diterpenes From the Roots of Acacia nilotica

2021 ◽  
Vol 9 ◽  
Author(s):  
John V. Anyam ◽  
Priscilla E. Daikwo ◽  
Marzuq A. Ungogo ◽  
Nwakaego E. Nweze ◽  
Ngozichukwuka P. Igoli ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Mass Spectrometric ◽  
Acacia Nilotica ◽  
Cross Resistance ◽  
Kidney Cells ◽  
Significant Activity ◽  
Active Fraction ◽  
Human Embryonic Kidney ◽  
Human Embryonic Kidney Cells ◽  
Modest Effect

The powdered roots of the medicinal plant Acacia nilotica were extracted with hexane and ethyl acetate, and the extracts were subjected to column chromatography for the isolation of potentially bioactive compounds and their screening against kinetoplastid pathogens. NMR and HREI mass spectrometric analyses identified two new diterpenes, characterized as 16, 19-dihydroxycassa-12-en-15-one (Sandynone, 1) and (5S, 7R, 8R, 9R, 10S, 13Z, 17S)-7,8:7,17:16,17-triepoxy-7,8-seco-cassa-13-ene (niloticane B, 2). The previously reported (5S,7R,8R,9R,10S) -(-)-7,8-seco-7, 8-oxacassa-13,15-diene-7,17-diol (3), (5S,7R,8R,9R,10S) -(-)-7,8-seco-7, 8-oxacassa-13,15-dien-7-ol-17-al (4), and (5S,7R,8R,9R,10S) -(-)-7,8-seco-7, 8-oxacassa-13,15-dien-7-ol (5) a, mixture of stigmasterol (6a) and sitosterol (6b), and lupeol (7) were also isolated. Several column fractions displayed significant activity against a panel of Trypanosoma and Leishmania spp., and from the most active fraction, compound 4 was isolated with high purity. The compound displayed high activity, particularly against T. brucei, T. evansi, and L. mexicana (0.88–11.7 µM) but only a modest effect against human embryonic kidney cells and no cross-resistance with the commonly used melaminophenyl arsenical and diamidine classes of trypanocides. The effect of compound 4 against L. mexicana promastigotes was irreversible after a 5-h exposure, leading to the sterilization of the culture between 24 and 48 h.

Characterization of the human oxytocin receptor stably expressed in 293 human embryonic kidney cells

Life Sciences ◽  
1995 ◽  
Vol 57 (24) ◽  
pp. 2253-2261 ◽  
Author(s):  
Jeffrey R. Jasper ◽  
C.Meacham Harrell ◽  
Julie A. O'Brien ◽  
Douglas J. Pettibone
Keyword(s):  
Oxytocin Receptor ◽  
Kidney Cells ◽  
Human Embryonic Kidney ◽  
Human Embryonic Kidney Cells

scholarly journals Functional Comparison between VP64-dCas9-VP64 and dCas9-VP192 CRISPR Activators in Human Embryonic Kidney Cells

2021 ◽  
Vol 22 (1) ◽  
pp. 397
Author(s):  
Nasir Javaid ◽  
Thuong L. H. Pham ◽  
Sangdun Choi
Keyword(s):  
Protein Level ◽  
Reference Genes ◽  
Mrna Level ◽  
High Specificity ◽  
Kidney Cells ◽  
Human Embryonic Kidney ◽  
Human Embryonic Kidney Cells ◽  
Sox2 Expression

Reversal in the transcriptional status of desired genes has been exploited for multiple research, therapeutic, and biotechnological purposes. CRISPR/dCas9-based activators can activate transcriptionally silenced genes after being guided by gene-specific gRNA(s). Here, we performed a functional comparison between two such activators, VP64-dCas9-VP64 and dCas9-VP192, in human embryonic kidney cells by the concomitant targeting of POU5F1 and SOX2. We found 22- and 6-fold upregulations in the mRNA level of POU5F1 by dCas9-VP192 and VP64-dCas9-VP64, respectively. Likewise, SOX2 was up-regulated 4- and 2-fold using dCas9-VP192 and VP64dCas9VP64, respectively. For the POU5F1 protein level, we observed 3.7- and 2.2-fold increases with dCas9-VP192 and VP64-dCas9-VP64, respectively. Similarly, the SOX2 expression was 2.4- and 2-fold higher with dCas9-VP192 and VP64-dCas9-VP64, respectively. We also confirmed that activation only happened upon co-transfecting an activator plasmid with multiplex gRNA plasmid with a high specificity to the reference genes. Our data revealed that dCas9-VP192 is more efficient than VP64-dCas9-VP64 for activating reference genes.

Fenton Reaction-Generated Advanced Oxidation Protein Products Induces Inflammation in Human Embryonic Kidney Cells

Inflammation ◽  
2016 ◽  
Vol 39 (4) ◽  
pp. 1285-1290 ◽  
Author(s):  
Guilherme Vargas Bochi ◽  
Vanessa Dorneles Torbitz ◽  
Roberto Christ Vianna Santos ◽  
Monica Cubillos-Rojas ◽  
José Luis Rosa López ◽  
...  
Keyword(s):  
Fenton Reaction ◽  
Advanced Oxidation ◽  
Kidney Cells ◽  
Human Embryonic Kidney ◽  
Advanced Oxidation Protein Products ◽  
Human Embryonic Kidney Cells
Sign in / Sign up

Export Citation Format

Share Document