scholarly journals Skin mucus proteome map of European sea bass (Dicentrarchus labrax)

PROTEOMICS ◽  
2015 ◽  
Vol 15 (23-24) ◽  
pp. 4007-4020 ◽  
Author(s):  
Héctor Cordero ◽  
Monica F. Brinchmann ◽  
Alberto Cuesta ◽  
José Meseguer ◽  
María A. Esteban
Fishes ◽  
2020 ◽  
Vol 5 (1) ◽  
pp. 10
Author(s):  
Diana Ceballos-Francisco ◽  
Alberto Cuesta ◽  
María Ángeles Esteban

Changes in different immune activities in the skin mucus of gilthead seabream (Sparus aurata L.) and European sea bass (Dicentrarchus labrax L.) specimens exposed to a constant light–dark photoperiod (12 h L:12 h D) were studied. Samples were collected at 08:00 (light on), 14:00, 20:00 (light off), 02:00, and again at 08:00 to determine immunoglobulin M (IgM) levels, several enzymes related to the immune system, and bactericidal activity. IgM levels were higher during the day in seabream and reached a minimum value at 20:00, but it was hardly affected in sea bass. No significant variations were recorded in the levels of protease and antiprotease. Peroxidase reached its maximum level in seabream at 02:00, the same time that it reached its minimum level in sea bass. Lysozyme showed little variation in seabream, but it was significantly lower at 14:00 than during the rest of the cycle in sea bass. Finally, different interspecific variations on bactericidal activity against Vibrio harveyi were recorded. The findings demonstrate that the immune parameters present in skin mucus of these important fish species are affected by the light–dark cycle and that there are substantial interspecies differences.


Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1684
Author(s):  
Francisca P. Martínez-Antequera ◽  
Juan A. Martos-Sitcha ◽  
Jose M. Reyna ◽  
Francisco J. Moyano

This study evaluated the use of Ulva ohnoi as an ingredient in feeds for aquaculture in three different experiments. Experiment 1 was oriented to confirm the negative effect of U. ohnoi on fish digestion. Experiment 2 assessed the effect on growth, feed efficiency, and immune status of juvenile sea bass (Dicentrarchus labrax) fed on diets including U. ohnoi, previously treated or not with carbohydrases used to partially hydrolyze indigestible polysaccharides. Experiment 3 was aimed to evaluate the potential protective effect of U. ohnoi on the oxidative status of sea bream (Sparus aurata) challenged by the consumption of a feed formulated with the oil fraction completely oxidized. Results show a negligible effect of U. ohnoi meal on protein digestion when included in feeds at levels of 10% or less. Moreover, results of growth and feed use evidenced the possibility of using up to 5% inclusion of algal meal in feeds without adverse effects on the zootechnical parameters, while the enzyme pretreatment was ineffective to improve its nutritional use. Finally, the inclusion of U. onhoi in feeds determined both an immunostimulatory effect, evidenced by an increase in skin mucus lysozyme in the two mentioned fish species, and a positive influence on the oxidative metabolism of seabream when fed on a diet including rancid oil.


Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1580
Author(s):  
Borja Ordóñez-Grande ◽  
Pedro M. Guerreiro ◽  
Ignasi Sanahuja ◽  
Laura Fernández-Alacid ◽  
Antoni Ibarz

The European sea bass (Dicentrarchus labrax) is a euryhaline marine teleost that can often be found in brackish and freshwater or even in hypersaline environments. Here, we exposed sea bass juveniles to sustained salinity challenges for 15 days, simulating one hypoosmotic (3‰), one isosmotic (12‰) and one hyperosmotic (50‰) environment, in addition to control (35‰). We analyzed parameters of skin mucus exudation and mucus biomarkers, as a minimally invasive tool, and plasma biomarkers. Additionally, Na+/K+-ATPase activity was measured, as well as the gill mucous cell distribution, type and shape. The volume of exuded mucus increased significantly under all the salinity challenges, increasing by 130% at 50‰ condition. Significantly greater amounts of soluble protein (3.9 ± 0.6 mg at 50‰ vs. 1.1 ± 0.2 mg at 35‰, p < 0.05) and lactate (4.0 ± 1.0 µg at 50‰ vs. 1.2 ± 0.3 µg at 35‰, p < 0.05) were released, with clear energy expenditure. Gill ATPase activity was significantly higher at the extreme salinities, and the gill mucous cell distribution was rearranged, with more acid and neutral mucin mucous cells at 50‰. Skin mucus osmolality suggested an osmoregulatory function as an ion-trap layer in hypoosmotic conditions, retaining osmosis-related ions. Overall, when sea bass cope with different salinities, the hyperosmotic condition (50‰) demanded more energy than the extreme hypoosmotic condition.


2019 ◽  
Vol 90 ◽  
pp. 317-327 ◽  
Author(s):  
Shay Ravid-Peretz ◽  
Angelo Colorni ◽  
Galit Sharon ◽  
Michal Ucko

Aquaculture ◽  
2021 ◽  
pp. 737257
Author(s):  
A. Samaras ◽  
A. Dimitroglou ◽  
S. Kollias ◽  
G. Skouradakis ◽  
I.E. Papadakis ◽  
...  

Chemosphere ◽  
2007 ◽  
Vol 67 (6) ◽  
pp. 1171-1181 ◽  
Author(s):  
L. Giari ◽  
M. Manera ◽  
E. Simoni ◽  
B.S. Dezfuli

2011 ◽  
Vol 75 (5) ◽  
pp. 643-649 ◽  
Author(s):  
Francesco Abbate ◽  
Maria Cristina Guerrera ◽  
Giuseppe Montalbano ◽  
Felix De Carlos ◽  
Alberto Álvarez Suárez ◽  
...  

Author(s):  
Malyka Galay-Burgos ◽  
Lynda Llewellyn ◽  
Constantinos C Mylonas ◽  
Adelino V.M Canario ◽  
Silvia Zanuy ◽  
...  

Author(s):  
Marinelle Espino ◽  
Harkaitz Eguiraun ◽  
Oihane Diaz de Cerio ◽  
José Antonio Carrero ◽  
Nestor Etxebarria ◽  
...  

AbstractFeeding 3.9 and 6.7 mg Hg/kg (Se/Hg molar ratios of 0.8 and 0.4, respectively) for 14 days negatively affected Dicentrarchus labrax growth and total DNTB- and thioredoxin-reductase (TrxR) activities and the transcription of four redox genes (txn1, gpx1, txnrd3, and txnrd2) in the liver, but a diet with 0.5 mg Hg/kg (Se/Hg molar ratio 6.6) slightly increased both reductase activities and the transcription of txn1, gpx1, and txnrd2. Feeding 6.7 mg Hg/kg for 53 days downregulated the genes of the thioredoxin system (txn1, txnrd3, and txnrd2) but upregulated gpx1, confirming the previously proposed complementarity among the antioxidant systems. Substitution of 20% of the feed by thawed white fish (hake) slightly counteracted the negative effects of Hg. The effects were not statistically significant and were dependent, in a non-linear manner, on the Se/Hg molar ratio of the feed but not on its Hg concentration. These results stress the need to consider the Se/Hg molar ratio of the feed/food when evaluating the toxicity of Hg.


Sign in / Sign up

Export Citation Format

Share Document