Molecular weight distribution studies. I. Radiation-induced polymerization of liquid α-methylstyrene

1975 ◽  
Vol 13 (6) ◽  
pp. 1271-1284 ◽  
Author(s):  
A. S. Chawla ◽  
Robert Y. M. Huang
1980 ◽  
Vol 25 (8) ◽  
pp. 1633-1638 ◽  
Author(s):  
Kouichi Yamaguchi ◽  
Hiromasa Watanabe ◽  
Takanobu Sugo ◽  
Terutaka Watanabe ◽  
Masaaki Takehisa ◽  
...  

1977 ◽  
Author(s):  
Grant H. Barlow

The determination of molecular weight distribution using the sedimentation equilibrium analysis developed for polymers by T. Scholte (J. Polymer Sei, 6, 111, 1968) has been adapted for heparin analysis. Pork mucosal heparin separated into molecular weight subfractions by gel filtration on Ultrogel AcA44 (L.K.B.) was used to test the validity and resolving power of the method. Results indicate that the method is able to differentiate molecular weight distribution satisfactorily. Comparisons have been made of molecular weight distribution of samples from different species, organs and manufacturers. Average molecular weights for most samples center around 15,000 Dal tons, but samples show considerable variation in their distribution data. Results suggest that variations between manufacturers is more pronounced than the specie and organ difference indicating the importance of the purification procedure.


2007 ◽  
Vol 76 (2) ◽  
pp. 215-222 ◽  
Author(s):  
M. Kožurková ◽  
L. Letavayová ◽  
E. Mišúrová

In the present work, the influence of gamma radiation (3 Gy) on DNA methylation in slowly proliferating liver tissue of adult male rats and their offspring was studied. Total methylation of DNA was determined on the basis of presence of 5-methylcytosine at the inner cytosine of the C-C-G-G sequence by using restriction enzymes, endonuclease Msp I and its isoschisomer Hpa II. It was found that in the liver of control animals, molecular weight distribution of both Msp I and Hpa II digestion products of DNA and the level of DNA methylation were influenced by age mainly between the 3rd and the 7th days of life. At this time, significant difference was found especially in products of cleavage with enzyme Msp I, where an increase in DNA fragments of medium molecular weight (1 - 7 kb) occurred. The changes in the molecular weight distribution of DNA digestion products in the offspring of control rats were accompanied by an increase in the level of total DNA methylation from 29.8% to 67%. In the offspring of irradiated males, the level of DNA methylation was higher in comparison with the offspring of non-irradiated control males, especially on the 3rd day after birth. We suppose that this finding is conjoined with a transgeneration transfer of radiation-induced genome damage.


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