RNA interference of tyrosine hydroxylase caused rapid mortality by impairing cuticle formation in Nilaparvata lugens (Hemiptera: Delphacidae)

2020 ◽  
Vol 76 (6) ◽  
pp. 2225-2232
Author(s):  
Shu H Liu ◽  
Bao J Yang ◽  
Ai Y Wang ◽  
Ju Luo ◽  
Jian Tang
Keyword(s):  
Rna Interference ◽  
Tyrosine Hydroxylase ◽  
Nilaparvata Lugens ◽  
Cuticle Formation

Feeding-based RNA interference of atrehalose phosphate synthasegene in the brown planthopper,Nilaparvata lugens

2010 ◽  
Vol 19 (6) ◽  
pp. 777-786 ◽  
Author(s):  
J. Chen ◽  
D. Zhang ◽  
Q. Yao ◽  
J. Zhang ◽  
X. Dong ◽  
...  
Keyword(s):  
Rna Interference ◽  
Brown Planthopper ◽  
Nilaparvata Lugens

RNA interference in Nilaparvata lugens (Homoptera: Delphacidae) based on dsRNA ingestion

2011 ◽  
Vol 67 (7) ◽  
pp. 852-859 ◽  
Author(s):  
Jie Li ◽  
Qiuhong Chen ◽  
Yongjun Lin ◽  
Tingru Jiang ◽  
Gang Wu ◽  
...  
Keyword(s):  
Rna Interference ◽  
Nilaparvata Lugens

scholarly journals NADPH Oxidase 5 Is Essential for Molting and Oviposition in a Rice Planthopper Nilaparvata lugens

Insects ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 642
Author(s):  
Lu-Yao Peng ◽  
Zhen-Wei Dai ◽  
Rui-Rui Yang ◽  
Zhen Zhu ◽  
Wei Wang ◽  
...  
Keyword(s):  
Rna Interference ◽  
Nadph Oxidase ◽  
Insect Pest ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Leaf Sheath ◽  
Whole Genome Sequence ◽  
First Choice ◽  
Nadph Oxidase 5

The brown planthopper Nilaparvata lugens is a typical monophagous insect herbivore that feeds exclusively on rice sap. This insect pest causes serious damage to rice crops throughout East Asian countries. Chemical control remains the first choice for managing N. lugens populations; however, the use of insecticides has given rise to planthopper resurgence and additional environmental risks. Nilaparvata lugens is a model insect of Hemiptera because its whole genome sequence has been elucidated and is susceptible to RNA interference. In this study, our findings revealed that a superoxide-generating gene, NADPH oxidase 5 (Nox5), is essential for molting and oviposition in a Hemipteran insect Nilaparvata lugens. Knockdown of Nox5 transcript levels by RNA interference in 2nd–5th-instar nymphs results in significantly lethal deficits in the molting transitions from nymph–nymph and nymph–adult. Nox5 knockdown leads to a reduction of hydrogen peroxide in female ovaries and failure of oviposition from the insect ovipositor into the rice leaf sheath. Here, we provide in vivo evidence demonstrating that Nox5 is a key enzyme for regulating molting and oviposition in this insect species.

scholarly journals Expression and RNA Interference of Ribosomal Protein L5 Gene in Nilaparvata lugens (Hemiptera: Delphacidae)

2017 ◽  
Vol 17 (3) ◽  
Author(s):  
Jiajun Zhu ◽  
Peiying Hao ◽  
Chaofeng Lu ◽  
Yan Ma ◽  
Yalin Feng ◽  
...  
Keyword(s):  
Rna Interference ◽  
Ribosomal Protein ◽  
Nilaparvata Lugens ◽  
Ribosomal Protein L5

scholarly journals GFAT and PFK genes show contrasting regulation of chitin metabolism in Nilaparvata lugens

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Cai-Di Xu ◽  
Yong-Kang Liu ◽  
Ling-Yu Qiu ◽  
Sha-Sha Wang ◽  
Bi-Ying Pan ◽  
...  
Keyword(s):  
Rna Interference ◽  
Pest Control ◽  
Mortality Rates ◽  
Nilaparvata Lugens ◽  
Chitin Synthesis ◽  
Chitin Synthesis Inhibitors ◽  
Expression Of Genes ◽  
Rnai Technology ◽  
Wing Bud

AbstractGlutamine:fructose-6-phosphate aminotransferase (GFAT) and phosphofructokinase (PFK) are enzymes related to chitin metabolism. RNA interference (RNAi) technology was used to explore the role of these two enzyme genes in chitin metabolism. In this study, we found that GFAT and PFK were highly expressed in the wing bud of Nilaparvata lugens and were increased significantly during molting. RNAi of GFAT and PFK both caused severe malformation rates and mortality rates in N. lugens. GFAT inhibition also downregulated GFAT, GNPNA, PGM1, PGM2, UAP, CHS1, CHS1a, CHS1b, Cht1-10, and ENGase. PFK inhibition significantly downregulated GFAT; upregulated GNPNA, PGM2, UAP, Cht2-4, Cht6-7 at 48 h and then downregulated them at 72 h; upregulated Cht5, Cht8, Cht10, and ENGase; downregulated Cht9 at 48 h and then upregulated it at 72 h; and upregulated CHS1, CHS1a, and CHS1b. In conclusion, GFAT and PFK regulated chitin degradation and remodeling by regulating the expression of genes related to the chitin metabolism and exert opposite effects on these genes. These results may be beneficial to develop new chitin synthesis inhibitors for pest control.

scholarly journals A Clip Domain Serine Protease Involved in Egg Production in Nilaparvata lugens: Expression Patterns and RNA Interference

Insects ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 378 ◽  
Author(s):  
Jia-min Wu ◽  
Rong-er Zheng ◽  
Rui-juan Zhang ◽  
Jin-liang Ji ◽  
Xiao-ping Yu ◽  
...  
Keyword(s):  
Rna Interference ◽  
Serine Protease ◽  
Serine Proteases ◽  
Egg Production ◽  
Developmental Stages ◽  
Fat Body ◽  
Expression Patterns ◽  
Nilaparvata Lugens ◽  
Immune Functions ◽  
Serine Protease Domain

Clip domain serine proteases play vital roles in various innate immune functions and in embryonic development. Nilaparvata lugens proclotting enzymes (NlPCEs) belong to this protease family. NlPCE1 was reported to be involved in innate immunity, whereas the role of other NlPCEs is unclear. In the present study, N. lugens proclotting enzyme-3 (NlPCE3) was cloned and characterized. NlPCE3 contains a signal peptide, a clip domain, and a trypsin-like serine protease domain. NlPCE3 was expressed in all tissues examined (gut, fat body, and ovary), and at all developmental stages. Immunofluorescence staining showed that NlPCE3 was mainly expressed in the cytoplasm and cytomembrane of follicular cells. Double stranded NlPCE3 RNA interference clearly inhibited the expression of NlPCE3, resulting in abnormal egg formation and obstruction of ovulation. These results indicate that NlPCE3 plays an important role in egg production in N. lugens.

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