Proteomics profiling of swine serum following lipopolysaccharide stimulation

2020 ◽  
Vol 34 (7) ◽  
Author(s):  
Zohra Olumee‐Shabon ◽  
Chaitali Chattopadhaya ◽  
Michael J. Myers
Keyword(s):  
Swine Serum

An Inhibitor Present in Calf Serum Which Prevents Growth of BHK21 Cells in Suspension Culture

1972 ◽  
Vol 10 (1) ◽  
pp. 137-152
Author(s):  
H. OTSUKA
Keyword(s):  
Suspension Culture ◽  
Calf Serum ◽  
Soft Agar ◽  
Transformed Cells ◽  
Ammonium Sulphate Precipitation ◽  
Serum Inhibitor ◽  
Swine Serum ◽  
Rna And Dna Synthesis ◽  
Rna And Dna ◽  
Do So

BHK21 cells do not form colonies in soft agar suspension culture in the presence of calf serum; but if swine serum is used instead they do so. In methylcellulose suspension culture supplemented with calf serum the cells synthesize very little RNA or DNA, but if swine serum is used there is a marked increase of syntheses. Calf serum was found to contain an inhibitor which prevents the induction, but not the continuation, of RNA and DNA synthesis in BHK21 cells. In contrast the growth of polyoma-virus-transformed cells is not affected by this serum inhibitor which has been partially purified from calf serum by ammonium sulphate precipitation followed by Sephadex G-200 column chromatography.

scholarly journals Molecular detection and clinical aspects of porcine circovirus 3 infection in pigs from Brazil

2020 ◽  
Vol 72 (5) ◽  
pp. 1731-1736
Author(s):  
A.C.M. Cruz ◽  
I.L.F. Rodrigues ◽  
A.E. Souza ◽  
F.B. Knackfuss ◽  
R.L. Silveira ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Porcine Circovirus ◽  
Southeastern Brazil ◽  
Clinical Aspects ◽  
Statistical Association ◽  
Serum Samples ◽  
Clinical Presentations ◽  
Porcine Circovirus 3 ◽  
Clinical Conditions ◽  
Swine Serum

ABSTRACT Porcine circovirus 3 (PCV-3) DNA has been detected in serum samples from apparently healthy pigs as well as pigs with different clinical conditions. Molecular detection of PCV-3 was observed in swine serum samples from Southeastern - Brazil using a nested PCR designed specifically for this study. The epidemiology and clinical aspects of PCV-3 infection were evaluated. The samples originated from 154 pigs of both genders from different production phases and with different clinical presentations, sampled from 31 pig farms visited between 2013 and 2018. In this study, PCV-3 was detected in 26.7% of samples from all populations across varying ages. Statistical association (P=0.0285) was observed only between animals with respiratory signs and PCV-3; no PCV-3-positive animal had diarrhea. No statistical association was observed between PCV-3 and age, or gender of the pigs. Because PCV-3 is a newly discovered virus, there is very little information about its epidemiology. We hope that these data can help in future studies investigating PCV-3 epidemiology.

scholarly journals Iron-Binding Protein of Swine Serum.

1947 ◽  
Vol 1 ◽  
pp. 770-776 ◽  
Author(s):  
C.-B Laurell ◽  
B. Ingelman
Keyword(s):  
Binding Protein ◽  
Iron Binding ◽  
Iron Binding Protein ◽  
Swine Serum

scholarly journals Modifying Influence of Swine Serum-induced Liver Fibrosis on Development of Preneoplastic Lesions in Rat Liver

1996 ◽  
Vol 87 (3) ◽  
pp. 240-246 ◽  
Author(s):  
Shigetsugu Wada ◽  
Toshio Kato ◽  
Mamoru Mutai ◽  
Keisuke Ozaki ◽  
Shuji Yamaguchi ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Rat Liver ◽  
Preneoplastic Lesions ◽  
Swine Serum

Liquid Chromatographic Determination of Furazolidone in Swine Serum and Avian Egg

1995 ◽  
Vol 78 (4) ◽  
pp. 1126-1130 ◽  
Author(s):  
Kenichi Yosheda ◽  
Fusao Kondo
Keyword(s):  
Chromatographic Determination ◽  
Accurate Method ◽  
Uv Detector ◽  
Drug Detection ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Swine Serum ◽  
Avian Egg

Abstract A rapid, simple, and accurate method for determination of furazolidone (FZ) in swine serum and avian egg using liquid chromatography (LC) with a 358 nm ultraviolet-visible spectrophotometric detector is described. After liquid–liquid extraction of sample with ethyl acetate using Extrelut-3, the extract is evaporated, redissolved in 40% acetonitrile, and injected directly into the chromatograph. The antibiotic can be analyzed within 30 min. Withinday recoveries for swine serum and avian egg spiked with FZ at 1 ppm were 90.0 and 88.1%, respectively, with coefficients of variation of 3.52 and 3.88%, respectively. Between days recoveries for the 1 ppm samples were 87.2 and 87.0%, with coefficients of variation of 3.10 and 4.29%, respectively. Determination of FZ also was performed by LC/mass spectrometry (MS) with an atmosphericpressure chemical-ionization interface (APCI) system. The LC/MS–APCI system is more applicable for qualitative analysis than quantitative analysis because the drug detection limit (about 0.1 μg/mL) is almost the same as that of the LC–UV detector.

scholarly journals Hepatic fibrosis produced in rats by repeated intraperitoneal injections of swine serum.

1984 ◽  
Vol 46 (3) ◽  
pp. 265-271 ◽  
Author(s):  
Kazuyuki KITAMURA ◽  
Akira YASOSHIMA ◽  
O.Hitoshi IWASAKI ◽  
Akihiro KOJIMA ◽  
Kunio DOI ◽  
...  
Keyword(s):  
Hepatic Fibrosis ◽  
Swine Serum

scholarly journals A New Cholinesterase in Swine Serum

Nature ◽  
1950 ◽  
Vol 166 (4225) ◽  
pp. 698-698 ◽  
Author(s):  
MILTON GJELHAUG LEVINE ◽  
ANITA A. SURAN
Keyword(s):  
Swine Serum

Factors influencing DNA synthesis and degradation present in swine serum and aortic tissue

1972 ◽  
Vol 16 (1) ◽  
pp. 54-70 ◽  
Author(s):  
K.E Fritz ◽  
J Jarmolych ◽  
A.S Daoud ◽  
T Peters
Keyword(s):  
Dna Synthesis ◽  
Aortic Tissue ◽  
Factors Influencing ◽  
Swine Serum ◽  
Synthesis And Degradation

SSGF-I, a potent growth-promoting substance for mammalian cells from swine serum

1989 ◽  
Vol 2 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Yasushi Shintani ◽  
Keiji Iwamoto ◽  
Kazuaki Kitano
Keyword(s):  
Mammalian Cells ◽  
Growth Promoting ◽  
Potent Growth ◽  
Swine Serum

scholarly journals Anti-Rennin Factor in Swine Serum. Part I

1962 ◽  
Vol 36 (9) ◽  
pp. 764-769 ◽  
Author(s):  
Masayoshi OEDA ◽  
Imae SAKAINO
Keyword(s):  
Swine Serum
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