scholarly journals Molecular detection and clinical aspects of porcine circovirus 3 infection in pigs from Brazil

2020 ◽  
Vol 72 (5) ◽  
pp. 1731-1736
Author(s):  
A.C.M. Cruz ◽  
I.L.F. Rodrigues ◽  
A.E. Souza ◽  
F.B. Knackfuss ◽  
R.L. Silveira ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Porcine Circovirus ◽  
Southeastern Brazil ◽  
Clinical Aspects ◽  
Statistical Association ◽  
Serum Samples ◽  
Clinical Presentations ◽  
Porcine Circovirus 3 ◽  
Clinical Conditions ◽  
Swine Serum

ABSTRACT Porcine circovirus 3 (PCV-3) DNA has been detected in serum samples from apparently healthy pigs as well as pigs with different clinical conditions. Molecular detection of PCV-3 was observed in swine serum samples from Southeastern - Brazil using a nested PCR designed specifically for this study. The epidemiology and clinical aspects of PCV-3 infection were evaluated. The samples originated from 154 pigs of both genders from different production phases and with different clinical presentations, sampled from 31 pig farms visited between 2013 and 2018. In this study, PCV-3 was detected in 26.7% of samples from all populations across varying ages. Statistical association (P=0.0285) was observed only between animals with respiratory signs and PCV-3; no PCV-3-positive animal had diarrhea. No statistical association was observed between PCV-3 and age, or gender of the pigs. Because PCV-3 is a newly discovered virus, there is very little information about its epidemiology. We hope that these data can help in future studies investigating PCV-3 epidemiology.

scholarly journals Self-assembly into virus–like particles of the recombinant capsid protein of porcine circovirus type 3 and its application on antibodies detection

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yu Wang ◽  
Gang Wang ◽  
Wei-Tong Duan ◽  
Ming-Xia Sun ◽  
Meng-Hang Wang ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Self Assembly ◽  
Recombinant Strain ◽  
Vaccine Development ◽  
Porcine Circovirus ◽  
Clinical Samples ◽  
Serum Samples ◽  
Virus Like Particles ◽  
E Coli ◽  
Swine Serum

AbstractPCV3 capsid protein (Cap) is an important antigen for diagnosis and vaccine development. To achieve high-level expression of recombinant PCV3 Cap in Escherichia coli (E. coli), the gene of wild-type entire Cap (wt-eCap) was amplified from clinical samples, and three optimized entire Cap (opti-eCap) and one optimized Cap deleted nuclear location signal (NLS) (opti-dCap) gene fragments encoding the same amino acid sequence with wt-eCap were synthesized based on the codon bias of E. coli. Those gene fragments were inserted into the pET30a expression vector. One recombinant strain with the highest expressed soluble eCap from four entire Cap (one wt-eCap and three opti-eCap) and one recombinant strain expressed opti-dCap were selected for further purification. The purified eCap and dCap were identified by transmission electron microscopy (TEM), a large number of round hollow particles with a diameter of 10 nm virus-like particles (VLPs) were observed in eCap, whereas irregular aggregation of proteins observed in dCap. After formation the VLPs were applied as a coating antigen to establish an indirect ELISA (I-ELISA) for detection of PCV3-specific antibody in swine serum. 373 clinical swine serum samples from China collected in 2019 were tested utilizing the VLP-based I-ELISA method under optimized conditions. To the best of our knowledge, this is the first report of self-assembly into VLPs of PCV3 recombinant Cap. Our results demonstrated that the VLP-based I-ELISA will be a valuable tool for detecting the presence of PCV3 antibodies in serum samples and will facilitate screening of large numbers of swine serum for clinical purposes.

scholarly journals Molecular detection and genomic characterization of porcine circovirus 3 in pigs from Northeast China

2018 ◽  
Vol 14 (1) ◽  
Author(s):  
Zhuo Ha ◽  
Chang-zhan Xie ◽  
Jin-feng Li ◽  
Shu-bo Wen ◽  
Ke-long Zhang ◽  
...  
Keyword(s):  
Northeast China ◽  
Molecular Detection ◽  
Porcine Circovirus ◽  
Genomic Characterization ◽  
Porcine Circovirus 3

scholarly journals Identification of Porcine circovirus type (PCV2) and type 3 (PCV3), Porcine 2 parvovirus (PPV) in swine by multiplex PCR test

2020 ◽  
Author(s):  
Thoai Kim Tran ◽  
Trang Thi Thanh Nguyen ◽  
Hiep Lai Xuan Vu ◽  
Phat Xuan Dinh
Keyword(s):  
Multiplex Pcr ◽  
Real Time ◽  
Porcine Circovirus Type ◽  
Rapid Diagnosis ◽  
Porcine Circovirus ◽  
Serum Samples ◽  
Ns1 Gene ◽  
Type 3 ◽  
Swine Serum

Abstract Background : Aiming to simultaneously detect three important viruses known to be involved in reproductive problems of sows, a multiplex PCR (mPCR) test was developed to provide rapid diagnosis of porcine circovirus type 2 and 3 (PCV2, PCV3) and to illustrate parvovirus (PPV) prevalence in sow herds. Methods : Three pairs of specific primers were designed to target PCV2 Cap gene, PCV3 Cap gene and PPV NS1 gene, with predicted mPCR products of 702 bp, 267 bp and 380 bp, respectively. Results : The detection limit of mPCR was 100 copies/ reaction per target gene. Sequencing of mPCR products performed with clinical serum samples accurately confirmed results. The mPCR was run against a panel of 94 swine serum samples whose infection status had been pre-determined by commercial real-time PCR kits. Overall, the mPCR results matched 100% with the real-time PCRs. Conclusions : The developed mPCR test functions successfully and can be used in routine rapid diagnosis of PCV2, PCV3 and PPV.

scholarly journals Porcine Circovirus 3 Detection in Aborted Fetuses and Stillborn Piglets from Swine Reproductive Failure Cases

Viruses ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 264
Author(s):  
Viviane Saporiti ◽  
Laura Valls ◽  
Jaime Maldonado ◽  
Mónica Perez ◽  
Florencia Correa-Fiz ◽  
...  
Keyword(s):  
High Viral Load ◽  
Reproductive Failure ◽  
Porcine Circovirus ◽  
Respiratory Syndrome Virus ◽  
Porcine Parvovirus ◽  
Reproductive Disease ◽  
Porcine Circovirus 3 ◽  
Clinical Conditions ◽  
Domestic Swine

Porcine circovirus 3 (PCV-3) has been widely detected in healthy and diseased pigs; among different pathologic conditions, the strongest evidence of association comes from reproductive disease cases. However, simple viral detection does not imply the causality of the clinical conditions. Detection of PCV-3 within lesions may provide stronger evidence of causality. Thus, this study aimed to assess the frequency of PCV-3 detection in tissues from fetuses/stillborn piglets in cases of reproductive problems in domestic swine, as well as the histopathologic assessment of fetal tissues. Fetuses or stillborn piglets from 53 cases of reproductive failure were collected and analyzed by PCV-3 qPCR. The presence of porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus 2 (PCV-2), and porcine parvovirus 1 (PPV1) was also checked. PCV-3 qPCR positive samples with a high viral load were tested by PCV-3 in situ hybridization (ISH), sequenced, and phylogenetically analyzed. PCV-3 DNA was detected in 18/53 (33.9%) reproductive failure cases and in 16 of them PCV-3 was the only pathogen found. PCV-2 DNA was found in 5/53 (9.4%), PRRSV RNA in 4/53 (7.5%) and PPV1 was not detected. Four out of the six PCV-3 qPCR-positive cases with Ct value <30 were positive when tested by ISH. In these samples, PCV-3 was detected within mild histopathologic lesions, such as arteritis and periarteritis in multiple tissues. The present work emphasizes the need to include PCV-3 as a potential causative agent of reproductive failure in swine.

scholarly journals Retrospective detection of Porcine circovirus 3 (PCV-3) in pig serum samples from Spain

2018 ◽  
Vol 65 (5) ◽  
pp. 1290-1296 ◽  
Author(s):  
F. Klaumann ◽  
G. Franzo ◽  
M. Sohrmann ◽  
F. Correa-Fiz ◽  
M. Drigo ◽  
...  
Keyword(s):  
Porcine Circovirus ◽  
Serum Samples ◽  
Porcine Circovirus 3 ◽  
Pig Serum

Molecular Characterization of Novel Porcine Circovirus 3 (PCV3) in Pig Populations in the North of Vietnam

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Nguyen Van Giap ◽  
Chung Hee Chun ◽  
Huynh Thi My Le ◽  
Cao Thi Bich Phuong ◽  
Vu Thi Ngoc ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Porcine Circovirus ◽  
The North ◽  
Porcine Circovirus 3

scholarly journals Porcine Gammaherpesviruses in Italian Commercial Swine Population: Frequent but Harmless

Pathogens ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 47
Author(s):  
Giovanni Franzo ◽  
Michele Drigo ◽  
Matteo Legnardi ◽  
Laura Grassi ◽  
Maria Luisa Menandro ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Clinical Signs ◽  
Northern Italy ◽  
Porcine Circovirus ◽  
Swine Industry ◽  
Positive Interaction ◽  
High Prevalence ◽  
Swine Population ◽  
Clinical Conditions

Differently from alpha- and betaherpesviruses affecting swine, interest in the recently discovered Suid gammaherpesvirus 3, Suid gammaherpesvirus 4, and Suid gammaherpesvirus 5, also known as porcine lymphotropic herpesviruses (PLHV-1, PLHV-2, and PLHV-3), has largely focused on their role as potential zoonotic agents in cases of xenotransplantation. However, their role as primary pathogens of swine or as co-factors for other lymphotropic infections has essentially been neglected. The present study aims at filling this gap, evaluating the association between PLHVs infection and different clinical conditions and/or porcine circovirus (PCV) co-infection. One hundred seventy-six samples were obtained from different animals located in a high-density pig area of Northern Italy in the period 2017–2020. The presence of PLHVs and PCVs was tested and quantified by specific real-time PCR: PLHVs were widespread among pigs (PLHV-1, PLHV-2, and PLHV-3 prevalence was 28.97%, 10.79%, and 4.54%, respectively) and detected in all considered tissues and clinical conditions. Frequent co-infections were also observed among PLHVs and with PCVs, although a significant association was not detected with the exception of a positive interaction between PLHV-1 and PLHV-3, and a negative one between PLHV-2 and PCV-2. Significantly, no association between PLHVs, alone or in co-infection, emerged with any of the considered clinical signs, their frequency being comparable between healthy and diseased animals. Based on these pieces of evidence and despite their high prevalence, PLHVs’ relevance for the swine industry appears negligible, either as primary pathogens or as predisposing factors for circovirus-induced diseases.

Molecular Detection of Coxiella burnetii in Unstandardized Minas Artisanal Cheese Marketed in Southeastern Brazil

Acta Tropica ◽  
2021 ◽  
pp. 105942
Author(s):  
Camila de Fátima Nascimento ◽  
Victória Valente Califre de Melo ◽  
Rosângela Zacarias Machado ◽  
Marcos Rogério André ◽  
Karina Paes Bürger
Keyword(s):  
Coxiella Burnetii ◽  
Molecular Detection ◽  
Southeastern Brazil
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