SSGF-I, a potent growth-promoting substance for mammalian cells from swine serum

1989 ◽  
Vol 2 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Yasushi Shintani ◽  
Keiji Iwamoto ◽  
Kazuaki Kitano
Keyword(s):  
Mammalian Cells ◽  
Growth Promoting ◽  
Potent Growth ◽  
Swine Serum

scholarly journals Reconstitution of dynamic microtubules with Drosophila XMAP215, EB1, and Sentin

2012 ◽  
Vol 199 (5) ◽  
pp. 849-862 ◽  
Author(s):  
Wenjing Li ◽  
Takashi Moriwaki ◽  
Tomomi Tani ◽  
Takashi Watanabe ◽  
Kozo Kaibuchi ◽  
...  
Keyword(s):  
Growth Rate ◽  
S2 Cells ◽  
Cooperative Action ◽  
Drosophila Melanogaster S2 Cells ◽  
Cargo Protein ◽  
Wide Range ◽  
Growth Promoting ◽  
Accelerated Growth ◽  
Potent Growth

Dynamic microtubules (MTs) are essential for various intracellular events, such as mitosis. In Drosophila melanogaster S2 cells, three MT tip-localizing proteins, Msps/XMAP215, EB1, and Sentin (an EB1 cargo protein), have been identified as being critical for accelerating MT growth and promoting catastrophe events, thus resulting in the formation of dynamic MTs. However, the molecular activity of each protein and the basis of the modulation of MT dynamics by these three factors are unknown. In this paper, we showed in vitro that XMAP215msps had a potent growth-promoting activity at a wide range of tubulin concentrations, whereas Sentin, when recruited by EB1 to the growing MT tip, accelerated growth and also increased catastrophe frequency. When all three factors were combined, the growth rate was synergistically enhanced, and rescue events were observed most frequently, but frequent catastrophes restrained the lengthening of the MTs. We propose that MT dynamics are promoted by the independent as well as the cooperative action of XMAP215msps polymerase and the EB1–Sentin duo.

Regenerating Bone Marrow Produces a Potent Growth-Promoting Activity to Osteogenic Cells*

Endocrinology ◽  
1988 ◽  
Vol 123 (1) ◽  
pp. 345-352 ◽  
Author(s):  
I. BAB ◽  
D. GAZIT ◽  
A. MUHLRAD ◽  
A. SHTEYER
Keyword(s):  
Bone Marrow ◽  
Osteogenic Cells ◽  
Growth Promoting ◽  
Potent Growth

Serum levels of TIMP-1 and MMP-7 as potential biomarkers in patients with metastatic colorectal cancer

2019 ◽  
Vol 34 (3) ◽  
pp. 292-301 ◽  
Author(s):  
Michal Vočka ◽  
Daniel Langer ◽  
Vladimir Fryba ◽  
Jaromir Petrtyl ◽  
Tomas Hanus ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Metastatic Colorectal Cancer ◽  
Carcinoembryonic Antigen ◽  
Serum Levels ◽  
Healthy Controls ◽  
Serum Samples ◽  
Gastrointestinal Malignancies ◽  
Matrix Metalloproteinase 7 ◽  
Growth Promoting ◽  
Potent Growth

Objective: Tissue inhibitor of metalloproteinases 1 (TIMP-1) and matrix metalloproteinase 7 (MMP-7) were reported to have potent growth promoting activity. Lack of balance between MMPs and TIMPs is an important factor in the development of gastrointestinal malignancies. Methods: We collected serum samples from 97 patients with metastatic colorectal cancer and 79 samples from healthy controls. Serum levels of TIMP-1 and MMP-7 were measured immunochemically and compared with standard tumor markers carcinoembryonic antigen and CA19-9. Results: Serum levels of TIMP-1 and MMP-7 were significantly higher in patients with colorectal cancer compared to healthy controls (both, P < 0.001). TIMP-1 and MMP-7 correlate with the presence of colon involvement (P = 0.001; P = 0.012) and the presence of liver metastases (P = 0.002; P = 0.037), and negatively correlate with pulmonary metastases (P = 0.014; P = 0.005). MMP-7 had similar sensitivity and the same specificity as carcinoembryonic antigen. TIMP-1 and MMP-7 had better sensitivity than CA19-9. TIMP-1 and MMP-7 level correlate with worse outcome (P = 0.002). Conclusion: The results indicate that TIMP-1 and MMP-7 are effective biomarkers in patients with metastatic colorectal cancer with good sensitivity. TIMP-1 and MMP-7 levels strongly correlate with the extent of liver disease and have prognostic value.

Effect of the growth-promoting alpha-globulin (GPAG) on in vitro incorporation of exogenous DNA into mammalian cells

1977 ◽  
Vol 33 (1) ◽  
pp. 33-34 ◽  
Author(s):  
J. Marec ◽  
Jarmila Keprtová ◽  
Věra Spurná ◽  
Eva Minářová ◽  
J. Michl
Keyword(s):  
Mammalian Cells ◽  
Exogenous Dna ◽  
Growth Promoting

scholarly journals Insulin resistance and hyperinsulinaemia in the development and progression of cancer

2009 ◽  
Vol 118 (5) ◽  
pp. 315-332 ◽  
Author(s):  
Ian F. Godsland
Keyword(s):  
Insulin Resistance ◽  
Cell Proliferation ◽  
Clinical Evidence ◽  
Causative Factor ◽  
Relative Importance ◽  
Subclinical Inflammation ◽  
Promote Cell Proliferation ◽  
Growth Promoting ◽  
Potent Growth ◽  
Interacting Disturbances

Experimental, epidemiological and clinical evidence implicates insulin resistance and its accompanying hyperinsulinaemia in the development of cancer, but the relative importance of these disturbances in cancer remains unclear. There are, however, theoretical mechanisms by which hyperinsulinaemia could amplify such growth-promoting effects as insulin may have, as well as the growth-promoting effects of other, more potent, growth factors. Hyperinsulinaemia may also induce other changes, particularly in the IGF (insulin-like growth factor) system, that could promote cell proliferation and survival. Several factors can independently modify both cancer risk and insulin resistance, including subclinical inflammation and obesity. The possibility that some of the effects of hyperinsulinaemia might then augment pro-carcinogenic changes associated with disturbances in these factors emphasizes how, rather than being a single causative factor, insulin resistance may be most usefully viewed as one strand in a network of interacting disturbances that promote the development and progression of cancer.

scholarly journals FOXO-regulated transcription restricts overgrowth of Tsc mutant organs

2008 ◽  
Vol 180 (4) ◽  
pp. 691-696 ◽  
Author(s):  
Kieran F. Harvey ◽  
Jaakko Mattila ◽  
Avi Sofer ◽  
F. Christian Bennett ◽  
Matthew R. Ramsey ◽  
...  
Keyword(s):  
Insulin Signaling ◽  
Mammalian Cells ◽  
Growth Regulation ◽  
Pathway Activity ◽  
Tor Pathway ◽  
Diverse Species ◽  
Elevated Expression ◽  
Transcriptional Changes ◽  
Growth Promoting ◽  
Inactivating Mutations

FOXO is thought to function as a repressor of growth that is, in turn, inhibited by insulin signaling. However, inactivating mutations in Drosophila melanogaster FOXO result in viable flies of normal size, which raises a question over the involvement of FOXO in growth regulation. Previously, a growth-suppressive role for FOXO under conditions of increased target of rapamycin (TOR) pathway activity was described. Here, we further characterize this phenomenon. We show that tuberous sclerosis complex 1 mutations cause increased FOXO levels, resulting in elevated expression of FOXO-regulated genes, some of which are known to antagonize growth-promoting pathways. Analogous transcriptional changes are observed in mammalian cells, which implies that FOXO attenuates TOR-driven growth in diverse species.

scholarly journals Recombinant novel pituitary adenylate cyclase-activating polypeptide from African catfish (Clarias gariepinus) authenticates its biological function as a growth-promoting factor in low vertebrates

2008 ◽  
Vol 197 (3) ◽  
pp. 583-597 ◽  
Author(s):  
Juana Maria Lugo ◽  
Alina Rodriguez ◽  
Yusmila Helguera ◽  
Reynold Morales ◽  
Osmany Gonzalez ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Mammalian Cells ◽  
Recombinant Expression ◽  
Clarias Gariepinus ◽  
Growth Control ◽  
Structural Evolution ◽  
African Catfish ◽  
Pituitary Adenylate Cyclase ◽  
Growth Promoting

Nowadays, the studies of pituitary adenylate cyclase-activating polypeptide (PACAP)-related peptide (PRP) and PACAP in non-mammalian vertebrates, especially in fish, have paid attention mainly to the localization, cloning, and structural evolution of the peptides, but very little is known about its biological functions as growth-promoting factors in low vertebrates. In this work, we have cloned and characterized the PRP/PACAP cDNA from the commercially important North African catfish Clarias gariepinus. The sequence obtained agrees with the higher conservation of PACAP than of PRP peptide sequences. We have reported for the first time the recombinant expression of fish PRP and PACAP in mammalian cells and bacteria and also demonstrated that the growth rate of fish is enhanced by both PRP and PACAP recombinant peptides. The results obtained in vivo in three different fish species, catfish (C. gariepinus), tilapia (Oreochromis niloticus), and carp (Cyprinus carpio) support the finding that PACAP rather than PRP plays a primordial role in growth control in teleost fish. This finding could help to elucidate the neuroendocrine axis proposed to explain the hypothalamic regulation of growth in non-mammalian vertebrates.

Growth promoting activity for mammalian cells in fractions of tissue extracts

1965 ◽  
Vol 38 (3) ◽  
pp. 523-535 ◽  
Author(s):  
M. Moskowitz ◽  
Dorothy M. Schenck
Keyword(s):  
Mammalian Cells ◽  
Tissue Extracts ◽  
Growth Promoting

Suspension Culture of Mammalian Cells and Macromolecular Growth-Promoting Fractions of Calf Serum

Nature ◽  
1964 ◽  
Vol 201 (4917) ◽  
pp. 375-378 ◽  
Author(s):  
B. T. TOZER ◽  
S. J. PIRT
Keyword(s):  
Suspension Culture ◽  
Mammalian Cells ◽  
Calf Serum ◽  
Growth Promoting
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