scholarly journals Robust deposition of lambda DNA on mica for imaging by AFM in air

Scanning ◽  
2014 ◽  
Vol 36 (6) ◽  
pp. 561-569 ◽  
Author(s):  
Nancy Anabel Gerling Cervantes ◽  
Braulio Gutiérrez- Medina
Keyword(s):  
Lambda Dna

scholarly journals Further tests of a recombination model in which chi removes the RecD subunit from the RecBCD enzyme of Escherichia coli.

Genetics ◽  
1990 ◽  
Vol 126 (3) ◽  
pp. 519-533 ◽  
Author(s):  
F W Stahl ◽  
L C Thomason ◽  
I Siddiqi ◽  
M M Stahl
Keyword(s):  
Escherichia Coli ◽  
Dna Sequence ◽  
Dna Packaging ◽  
Lambda Phage ◽  
Recombination Model ◽  
Phage Types ◽  
Point Of Entry ◽  
Reciprocal Recombination ◽  
Recbcd Enzyme ◽  
Lambda Dna

Abstract When one of two infecting lambda phage types in a replication-blocked cross is chi + and DNA packaging is divorced from the RecBCD-chi interaction, complementary chi-stimulated recombinants are recovered equally in mass lysates only if the chi + parent is in excess in the infecting parental mixture. Otherwise, the chi 0 recombinant is recovered in excess. This observation implies that, along with the chi 0 chromosome, two chi + parent chromosomes are involved in the formation of each chi + recombinant. The trimolecular nature of chi +-stimulated recombination is manifest in recombination between lambda and a plasmid. When lambda recombines with a plasmid via the RecBCD pathway, the resulting chromosome has an enhanced probability of undergoing lambda x lambda recombination in the interval into which the plasmid was incorporated. These two observations support a model in which DNA is degraded by Exo V from cos, the sequence that determines the end of packaged lambda DNA and acts as point of entry for RecBCD enzyme, to chi, the DNA sequence that stimulates the RecBCD enzyme to effect recombination. The model supposes that chi acts by ejecting the RecD subunit from the RecBCD enzyme with two consequences. (1) ExoV activity is blocked leaving a highly recombinagenic, frayed duplex end near chi, and (2) as the enzyme stripped of the RecD subunit travels beyond chi it is competent to catalyze reciprocal recombination.

scholarly journals Domains for protein-protein interactions at the N and C termini of the large subunit of bacteriophage lambda terminase.

Genetics ◽  
1988 ◽  
Vol 119 (3) ◽  
pp. 477-484
Author(s):  
W F Wu ◽  
S Christiansen ◽  
M Feiss
Keyword(s):  
Protein Interactions ◽  
Ternary Complex ◽  
Large Subunit ◽  
Small Subunit ◽  
Functional Domain ◽  
Binary Complex ◽  
Protein Protein Interactions ◽  
Related Phage ◽  
Carboxy Terminal ◽  
Lambda Dna

Abstract The large subunit of phage lambda terminase, gpA, the gene product of the phage A gene, interacts with the small subunit, gpNul, to form functional terminase. Terminase binds to lambda DNA at cosB to form a binary complex. The terminase:DNA complex binds a prohead to form a ternary complex. Ternary complex formation involves an interaction of the prohead with gpA. The amino terminus of gpA contains a functional domain for interaction with gpNul, and the carboxy-terminal 38 amino acids of gpA contain a functional domain for prohead binding. This information about the structure of gpA was obtained through the use of hybrid phages resulting from recombination between lambda and the related phage 21. lambda and 21 encode terminases that are analogous in structural organization and have ca. 60% sequence identity. In spite of these similarities, lambda and 21 terminases differ in specificity for DNA binding, subunit assembly, and prohead binding. A lambda-21 hybrid phage produces a terminase in which one of the subunits is chimeric and had recombinant specificities. In the work reported here; a new hybrid, lambda-21 hybrid 67, is characterized. lambda-21 hybrid 67 is the result of a crossover between lambda and 21 in the large subunit genes, such that the DNA from the left chromosome end is from 21, including cosB phi 21, the 1 gene, and the first 48 codons for the 2 gene. The rest of the hybrid 67 chromosome is lambda DNA, including 593 codons of the A gene. The chimeric gp2/A of hybrid 67 binds gp1 to form functional terminase.(ABSTRACT TRUNCATED AT 250 WORDS)

Factors which influence CaCl2 dependent transfection of Lambda DNA in Escherichia coli K12 recipients

1980 ◽  
Vol 20 (4) ◽  
pp. 271-281 ◽  
Author(s):  
M. Pfeifer ◽  
Ch. Pöhlmann ◽  
A. Betcke ◽  
M. Kurth ◽  
D.-H. Liebscher
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli K12 ◽  
Lambda Dna

scholarly journals Formation of transmembrane channels in liposomes during injection of lambda DNA.

1986 ◽  
Vol 261 (1) ◽  
pp. 386-390
Author(s):  
C A Roessner ◽  
G M Ihler
Keyword(s):  
Transmembrane Channels ◽  
Lambda Dna

scholarly journals Digestion of 5-bromodeoxyuridine-substituted lambda-DNA by restriction endonucleases.

1978 ◽  
Vol 253 (24) ◽  
pp. 9075-9081
Author(s):  
M.A. Marchionni ◽  
R.J. Roufa
Keyword(s):  
Restriction Endonucleases ◽  
Lambda Dna

On the structure of the ends of lambda DNA

1965 ◽  
Vol 12 (1) ◽  
pp. 36-49 ◽  
Author(s):  
H.B. Strack ◽  
A.D. Kaiser
Keyword(s):  
Lambda Dna

scholarly journals A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction

2000 ◽  
Vol 23 (1) ◽  
pp. 65-66 ◽  
Author(s):  
Roberto V. Santelli ◽  
Luci Deise Navarro-Cattapan
Keyword(s):  
Gradient Centrifugation ◽  
Enzymatic Treatment ◽  
Dna Purification ◽  
Phenol Extraction ◽  
Cscl Gradient ◽  
Centrifugation Method ◽  
Lambda Dna ◽  
Tedious Process

A modification of the CsCl gradient centrifugation method for DNA phage purification is presented. It avoids the enzymatic steps as well the need for a preliminary phage titration, a tedious process proposed in the majority of the protocols in use. The quality of the DNA obtained makes it amenable for additional manipulations like digestions, ligations, labelling, subcloning, etc.

scholarly journals Nucleosome Positioning on Lambda DNA for Single-Molecule Analysis of Chromatin Remodeling - Development of a Versatile Lambda DNA Construct Capable of Reception of any DNA Sequences of Interest

2014 ◽  
Vol 106 (2) ◽  
pp. 70a
Author(s):  
Mate Gyimesi ◽  
Jody L. Plank ◽  
Jason C. Bell ◽  
James E. Graham ◽  
Christopher C. Dombrowski ◽  
...  
Keyword(s):  
Chromatin Remodeling ◽  
Single Molecule ◽  
Dna Sequences ◽  
Nucleosome Positioning ◽  
Single Molecule Analysis ◽  
Lambda Dna
Sign in / Sign up

Export Citation Format

Share Document