scholarly journals Hemopoietic progenitor cells in the blood as indicators of the functional status of the bone marrow after total-body and partial-body irradiation: Experiences from studies in dogs

Stem Cells ◽  
2009 ◽  
Vol 16 (S2) ◽  
pp. 97-111 ◽  
Author(s):  
Wilhelm Nothdurft ◽  
Ludwika Kreja
Keyword(s):  
Bone Marrow ◽  
Functional Status ◽  
Progenitor Cells ◽  
Partial Body ◽  
Total Body ◽  
Hemopoietic Progenitor ◽  
Hemopoietic Progenitor Cells

PO8-190 TRANSDUCTION OF BONE MARROW HEMOPOIETIC PROGENITOR CELLS WITH HIV-BASED PSEUDOVIRAL PARTICLES EX VIVO

2007 ◽  
Vol 8 (1) ◽  
pp. 64
Author(s):  
N. Radukhina ◽  
O. Ilyinskaya ◽  
A. Kozlov ◽  
P. Rutkevich ◽  
T. Vlasik ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Ex Vivo ◽  
Hemopoietic Progenitor ◽  
Hemopoietic Progenitor Cells

In vivo Stimulatory Effects of Neopterin on Hematopoiesis

Pteridines ◽  
1998 ◽  
Vol 9 (1) ◽  
pp. 13-17 ◽  
Author(s):  
Shin Aizawa ◽  
Masaki Hiramoto ◽  
Sonoko Araki ◽  
Hajime Hosh ◽  
Shuji Kojima ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Intraperitoneal Administration ◽  
Extensive Study ◽  
Blood Leukocytes ◽  
Proliferation And Differentiation ◽  
Blood Leukocyte ◽  
Hemopoietic Progenitor ◽  
Hemopoietic Progenitor Cells

Summary The pteridine neopterin (NP) was shown to be produced by monocytes and has been known as a useful maker of immunological activation, although, the biological activity of this agent is still unclear. To elucidate the biological function of NP, the changes in the number of blood leukocyte, bone marrow and spleen hemopoietic progenitor cells after intraperitoneal administration of NP into mice were investigated. Administration of NP increased the number of blood leukocytes about 2 fold higher than that of the control at days 7 and 14. Blood films, made by smearing samples of peripheral blood, showed the increment of granulocytes in blood of NP treated animals. The number of granulocyte-macrophage progenitor cells (CFU-GM) was also increased in both bone marrow and spleen in mice with NP administration. Extensive study showed that NP stimulated the hematological recovery in bone marrow transplanted animals. All these findings suggest that NP has a stimulating activity on hemopoiesis by affecting the proliferation and differentiation of hemopoietic progenitor cells in vivo.

scholarly journals Selective resistance of bone marrow-derived hemopoietic progenitor cells to gliotoxin.

1987 ◽  
Vol 84 (11) ◽  
pp. 3822-3825 ◽  
Author(s):  
A. Mullbacher ◽  
D. Hume ◽  
A. W. Braithwaite ◽  
P. Waring ◽  
R. D. Eichner
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Hemopoietic Progenitor ◽  
Hemopoietic Progenitor Cells

CD34+ Human Hemopoietic Progenitor Cells of the Bone Marrow Differ from Those of the Peripheral Blood: An Immunocytochemical and Morphometric Study

1995 ◽  
Vol 93 (2-4) ◽  
pp. 83-90 ◽  
Author(s):  
Claudia Wickenhauser ◽  
Jürgen Thiele ◽  
Uta Drebber ◽  
Hans Michael Kvasnicka ◽  
Andreas Thiel ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Peripheral Blood ◽  
Morphometric Study ◽  
Hemopoietic Progenitor ◽  
Hemopoietic Progenitor Cells

scholarly journals Antibody stimulation of hemopoietic progenitor cells

Blood ◽  
1985 ◽  
Vol 65 (4) ◽  
pp. 869-876 ◽  
Author(s):  
RM Crapper ◽  
JW Schrader
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Bone Marrow Cells ◽  
Exchange Chromatography ◽  
Staphylococcal Protein A ◽  
Igg Antibodies ◽  
Hemopoietic Progenitor ◽  
Hemopoietic Progenitor Cells ◽  
Stimulation Of

Abstract Antisera were raised by immunizing rabbits with cloned lines of murine hemopoietic progenitor cells (P cells) that depended on the presence of a specific hemopoietic growth factor, persisting cell-stimulating factor (PSF), for their growth and survival. The unabsorbed antiserum was inhibitory, but after absorption with murine spleen cells and the mastocytoma, P815, significant stimulation of both P cell growth and thymidine incorporation was evident. IgG antibodies isolated from the antiserum by staphylococcal protein A chromatography or further purified by diethylaminoethyl anion exchange chromatography, ammonium sulphate precipitation, and gel filtration using Sephacryl S-300 were responsible for the stimulation. The absorbed antiserum promoted the survival of normal murine bone marrow cells in liquid culture over a four-day period, and the inclusion of IgG antibodies in agar cultures of normal bone marrow promoted the in vitro survival, over a 48-hour period, of cells capable of subsequently generating, in the presence of a source of PSF, colonies of neutrophils, macrophages, and megakaryocytes. It is postulated that the antibodies act by stimulating the PSF receptor on both the factor-dependent cell lines and normal myeloid progenitor cells.

scholarly journals Near-optimal Conditions for the In Vitro Culture of Hemopoietic Progenitor Cells in Bone Marrow from the Rat

2009 ◽  
Vol 37 (2) ◽  
pp. 170-174 ◽  
Author(s):  
Gemma Molyneux ◽  
Sian Rizzo ◽  
John Turton ◽  
Parvinder Phul ◽  
Frances Gibson
Keyword(s):  
Bone Marrow ◽  
In Vitro Culture ◽  
Progenitor Cells ◽  
Optimal Conditions ◽  
Hemopoietic Progenitor ◽  
Hemopoietic Progenitor Cells

scholarly journals Expression and function of c-kit in hemopoietic progenitor cells.

1991 ◽  
Vol 174 (1) ◽  
pp. 63-71 ◽  
Author(s):  
M Ogawa ◽  
Y Matsuzaki ◽  
S Nishikawa ◽  
S Hayashi ◽  
T Kunisada ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Bone Marrow Cells ◽  
Marrow Cell ◽  
Adult Bone Marrow ◽  
Lineage Markers ◽  
And Function ◽  
Adult Bone ◽  
Hemopoietic Progenitor ◽  
Hemopoietic Progenitor Cells

The expression and function of a receptor tyrosine kinase, c-kit, in the adult bone marrow of the mouse were investigated by using monoclonal antibodies (mAbs) against the extracellular domain of murine c-kit. In adult C57BL/6 mouse, 7.8% of total bone marrow cells express c-kit on their surface. Half of the c-kit+ cells do not express lineage markers including Mac-1, Gr-1, TER-119, and B220, while the remainder coexpress myeloid lineage markers such as Mac-1 and Gr-1. After c-kit+ cells were removed from the bone marrow cell preparation, hemopoietic progenitor cells reactive to IL-3, GM-CSF, or M-CSF and also those which give rise to spleen colonies in irradiated recipients disappeared almost completely. Thus, most hemopoietic progenitors in the adult bone marrow express c-kit. To investigate whether or not c-kit has any role in the hemopoiesis of adult bone marrow, we took the advantage of one of the anti-c-kit mAbs that can antagonize the function of c-kit. As early as two days after the injection of 1 milligram of an antagonistic antibody, ACK2, almost all hemopoietic progenitor cells disappeared from the bone marrow, which eventually resulted in the absence of mature myeloid and erythroid cells in the bone marrow. These results provide direct evidence that c-kit is an essential molecule for constitutive intramarrow hemopoiesis, especially for the self-renewal of hemopoietic progenitor cells at various stages of differentiation.

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