Distinct pharmacological regulation of evoked dopamine efflux in the amygdala and striatum of the rat in vivo

Synapse ◽  
1995 ◽  
Vol 20 (3) ◽  
pp. 269-279 ◽  
Author(s):  
Paul A. Garris ◽  
R. Mark Wightman
Keyword(s):  
Pharmacological Regulation ◽  
Dopamine Efflux

Dopamine transporters are involved in the onset of hypoxia-induced dopamine efflux in striatum as revealed by in vivo microdialysis

2005 ◽  
Vol 46 (8) ◽  
pp. 623-633 ◽  
Author(s):  
Cyrille Orset ◽  
Sandrine Parrot ◽  
Valérie Sauvinet ◽  
Jean-Marie Cottet-Emard ◽  
Anne Bérod ◽  
...  
Keyword(s):  
In Vivo Microdialysis ◽  
Dopamine Transporters ◽  
Dopamine Efflux

Subchronic methamphetamine treatment enhances methamphetamine- or cocaine-induced dopamine efflux in vivo

1989 ◽  
Vol 25 (7) ◽  
pp. 903-912 ◽  
Author(s):  
Yasuko Kazahaya ◽  
Kiyoshi Akimoto ◽  
Saburo Otsuki
Keyword(s):  
Dopamine Efflux

Effects of clozapine on the efflux of serotonin and dopamine in the rat brain: the role of 5-HT1A receptors

2002 ◽  
Vol 80 (12) ◽  
pp. 1158-1166 ◽  
Author(s):  
Yoko Hagino ◽  
Masayuki Watanabe
Keyword(s):  
Prefrontal Cortex ◽  
Dorsal Raphe Nucleus ◽  
Dorsal Raphe ◽  
In Vivo Microdialysis ◽  
Raphe Nucleus ◽  
Systemic And Local Administration ◽  
Dopamine Efflux ◽  
Dorsal Raphé

In vivo microdialysis in conscious rats was used to examine the effect of clozapine on serotonin (5-hydroxy tryptamine, 5-HT) efflux in the prefrontal cortex and dorsal raphe nucleus and dopamine efflux in the prefrontal cortex. Both systemic and local administration of clozapine (systemic,10 or 20 mg/kg, i.p.; local, 100 μM) increased 5-HT efflux in the dorsal raphe. However, in the prefrontal cortex, dialysate 5-HT increased when clozapine (100 μM) was administered through the probe, while no effect was observed when it was administered systemically. By pretreatment with the selective 5-HT1A receptor antagonist p-MPPI (3 mg/kg, i.p.), systemic treatment of clozapine (10 mg/kg, i.p.) significantly increased 5-HT efflux in the prefrontal cortex. This result suggests that the ability of clozapine to enhance the extracellular concentrations of 5-HT in the dorsal raphe attenuates this drug's effect in the frontal cortex, probably through the stimulation of 5-HT1A somatodendritic autoreceptors in the dorsal raphe. We also found that pretreatment with p-MPPI (3 mg/kg, i.p.) attenuated by 45% the rise in cortical dopamine levels induced by clozapine (10 mg/kg, i.p.). These findings imply that the reduction in serotonergic input from the dorsal raphe nucleus induced by clozapine could lead to an increase in dopamine release in the prefrontal cortex. Key words: 5-HT1A receptor, clozapine, microdialysis, 5-HT, dopamine.

Strain-specific facilitation of dopamine efflux by Δ9-tetrahydrocannabinol in the nucleus accumbens of rat: An in vivo microdialysis study

1991 ◽  
Vol 129 (1) ◽  
pp. 136-140 ◽  
Author(s):  
Jianping Chen ◽  
William Paredes ◽  
Joyce H. Lowinson ◽  
Eliot L. Gardner
Keyword(s):  
Nucleus Accumbens ◽  
In Vivo Microdialysis ◽  
Microdialysis Study ◽  
Dopamine Efflux

scholarly journals Multimodal Detection of Dopamine by Sniffer Cells Expressing Genetically Encoded Fluorescence Sensors

2021 ◽  
Author(s):  
Carmen Klein Herenbrink ◽  
Jonatan Fullerton Stoier ◽  
William Dalseg Reith ◽  
Abeer Dagra ◽  
Miguel Alejandro Cuadrado Gregorek ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Striatal Slices ◽  
Brain Functions ◽  
Endogenous Dopamine ◽  
Dopamine Content ◽  
Neuropsychiatric Diseases ◽  
Sensor Properties ◽  
Dopamine Efflux

Dopamine serves an important role in supporting both locomotor control and higher brain functions such as motivation and learning. Dopaminergic dysfunction is implicated in an equally multidimensional spectrum of neurological and neuropsychiatric diseases. Extracellular dopamine levels are known to be tightly controlled by presynaptic dopamine transporters (DAT), which is also a main target of psychostimulants. Still, detailed data on dopamine dynamics in space and time is needed to fully understand how dopamine signals are encoded and translated into cellular and behavioral responses, and to uncover the pathological effects of dopamine-related diseases. The recently developed genetically encoded fluorescent dopamine sensors enable unprecedented monitoring of dopamine dynamics and have changed the field of in vivo dopamine recording. However, the potential of these sensors to be used for in vitro and ex vivo assays remains unexplored. Here, we demonstrate a generalizable blueprint for making dopamine 'sniffer' cells for multimodal detection of dopamine in vitro and ex vivo. We generated sniffer cell lines with inducible expression of six different dopamine sensors and performed a head-to-head comparison of sensor properties to guide users in sensor selection. In proof-of-principle experiments, we show how the sniffer cells can be applied to measure release of endogenous dopamine from cultured neurons and striatal slices, and for determining total dopamine content in striatal tissue. Furthermore, we use the sniffer cells to quantify DAT-mediated dopamine uptake, and AMPH-induced and constitutive dopamine efflux as a radiotracer free, high-throughput alternative to electrochemical- and radiotracer-based assays. Importantly, the sniffer cells framework can readily be applied to other transmitter systems for which the list of genetically encoded fluorescent sensors is rapidly growing.

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