Effects of mobile phone electromagnetic radiation on rat hippocampus proteome

Objectives: Nowadays, mobile phones have become a prime necessity. At present, various advance networking connections such as 3G and 4G are available in the market to get a faster service. However, these mobile phones emit harmful electromagnetic radiations which have various ill effects on different physiological systems. The present study has been done to find out the extent of damage caused by mobile phone radiation on liver cells, and, whether high-protein diet (HPD) has any ameliorative effect on such changes. Materials and Methods: Male Swiss Albino mice were divided into four groups. Two groups of animals were exposed to mobile phone radiation at global system for mobile communications like frequencies at 1.8 GHz for 3 h continuously per day for 90 days. One of these radiation exposed groups received a normal diet (containing 5% casein) and the other exposed group received a HPD (supplemented with 20% casein). The control group animals received only a normal diet, while the animals of the remaining group received only a HPD. The serum biochemical and histological parameters of liver tissues and ladder assay of hepatic DNA were studied. Results: A significantly elevated level (P < 0.05) of aspartate aminotransferase, alanine transaminase, bilirubin and serum caspase-3 levels was observed in the group of animals exposed to electromagnetic radiation emitted from mobile phone. Histological changes were also observed in the experimental groups. Moreover, in genomic DNA ladder assay, fragmented DNA was observed in exposed group, which is a probable sign of cell apoptosis. Recovery symptoms were observed in the animals supplemented with high casein diet. Conclusion: From the present study, it may be concluded that electromagnetic radiation emitted from mobile phones has damaging effects on hepatocytes which may be ameliorated by HPD.

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THE IMPACT OF ELECTROMAGNETIC RADIATION OF MOBILE PHONE FOR LYMPHOCYTE ACTIVATION IN VITRO

Hygiene and Sanitation ◽

10.18821/0016-9900-2017-96-10-965-970 ◽

2019 ◽

Vol 96 (10) ◽

pp. 965-970 ◽

Cited By ~ 1

Author(s):

Maria S. Blyakher ◽

E. A. Tulskaya ◽

I. V. Kapustin ◽

I. M. Fedorova ◽

T. K. Lopatina ◽

...

Keyword(s):

Electromagnetic Radiation ◽

Mobile Phone ◽

Blood Cells ◽

Cytokine Production ◽

Venous Blood ◽

Peripheral Blood Lymphocytes ◽

Mean Values ◽

Healthy Donors ◽

The Impact

The character of the influence of the electromagnetic radiation (EMR) of mobile phone on the activation of lymphocytes in vitro was investigated. This is important, since modern human is exposed to a complex combination of electric and magnetic fields (EMF) of different frequencies. The object of the study were whole venous blood and lymphocytes isolated from 21 adult donors (aged of from 20 to 55 years) - 10 were healthy donors and 11 were healthy persons 7 days after their vaccination with meningococcal polysaccharide vaccine. In the study the influence of phone’s EMR on the functional activity of peripheral blood lymphocytes was determined by the flow cytometry method with the use of monoclonal antibodies of Beckman Coulter company (by the identification and calculation the number of basic and activated lymphocyte subpopulations). The changes of cytokines production by blood cells exposed to mobile phone electromagnetic radiation were determined in supernatants by measuring their concentration using EIA kits produced by JSC “Vector-Best” (Russia) and LLC “cytokine” (Russia). The results of the study of the effects of electromagnetic radiation of mobile phone on blood cells revealed changes in the percentage of lymphocytes carrying the early activation marker CD69 significantly to be more frequently and were observed with greater intensity in the group of donors which were vaccinated compared to healthy donors. Under the influence of phone’s EMR mean values of cytokine production determined in the supernatants samples did not changed in both groups, but in the group of healthy donors mean values of cytokines production were 1,5 - 2 times higher than in the group of persons following immunization. The increase or decrease in cytokine production under the influence of phone’s EMR occurred regardless of the initial level of its production in the surveyed donor. The changes of the cytokine production (IFNγ, TNFα, IL-6 and IL-8) by blood cells under the influence of phone’s EMR happen individually; this should be considered when deciding on the presence or absence of phone’s EMR impact on the status of lymphocytes.

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Mode of use by mobile phone and change of time of simple audio-motor reaction for users of mobile communication. Age related features of ipsilateral and contralateral effects

Biomedical Radioelectronics ◽

10.18127/j15604136-202101-05 ◽

2021 ◽

Author(s):

N.I. Khorseva ◽

O.R. Al’-Kudri ◽

P.E. Grigoryev ◽

R.I. Islyamov ◽

N.Yu. Shulzhenko

Keyword(s):

Electromagnetic Radiation ◽

Mobile Phone ◽

Auditory System ◽

Age Groups ◽

Control Group ◽

Age Group ◽

Motor Reaction ◽

Age Related ◽

Contralateral Effect ◽

Contralateral Effects

By analysing the time of a simple auditory-motor reaction with mono-presentation of an audio signal, the effect of the electromagnetic radiation of a mobile phone on the human auditory system is evaluated. Regularities in the frequency of occurrence of ipsi and contralateral effects in mobile users of different age groups are obtained. This study has no analogues in either Russian or foreign publications and is currently exclusive. The purpose was to study age-related features of the severity of ipsi and contralateral effects, depending on the mode of use of the mobile phone. To record the ipsi- and contralateral effects, we used the differences in the average values of a simple auditory-motor reaction between the left and right ears with mono-presentation of the sound signal of 573 respondents of different age groups. The obtained values were compared with the control group data for each age group (491 respondents). If the difference was greater than in the control group, the ipsilateral or contralateral effect was recorded. The severity of the effect was evaluated taking into account the lateral preferences of each participant in the experiments (to which ear the mobile phone is most often held) and the mode of use of the mobile phone. Statistical processing of the results was carried out using the non-parametric Mann-Whitney test and the method of descriptive statistics: prevalence indicator (Pr) (relative frequency of manifestation of a particular attribute in the sample). The comparison of the severity of ipsi and contralateral effects for different age groups allowed us to establish the following patterns. It was found that, in general, the manifestation of the contralateral effect decreases with age, and the ipsilateral effect increases. However, this change is not linear. It is shown that the features of the manifestation of the contralateral and ipsilateral effect for each age group depend both on the time of daily use of a mobile phone and on the total duration of use of a mobile phone. We believe that when studying multidimensional input data, an individual approach is necessary. The presented results confirm the negative effect of the radiation of mobile phones on the auditory system, primarily of our younger generation. Given the special vulnerability of children to physical environmental factors and the depth of penetration of EMR MT into the brain of a child, we believe that from a radiobiological point of view, there is already a need to develop a special SanPiN (sanitary rules and norms) for all available modern low-intensity sources of electromagnetic radiation, including Wi-Fi.

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The possible mechanisms of electromagnetic phone effects on the auditory system of children and adolescents

Biomedical Radioelectronics ◽

10.18127/j15604136-202103-02 ◽

2021 ◽

Author(s):

N.I. Khorseva ◽

P.E. Grigoriev

Keyword(s):

Electromagnetic Radiation ◽

Mobile Phone ◽

Children And Adolescents ◽

Auditory System ◽

Motor Response ◽

Pearson Correlation ◽

Motor Reaction ◽

The Right ◽

Contralateral Effects ◽

Daily Time

For the first time, we compared the gradients of changes in the simple auditory-motor response (SAMR) and the daily time of using a mobile phone (MP), which made it possible to describe the variants of possible changes in lateralization of effects and the frequency of their occurrence for different variants of SAMR changes. This study has no analogues neither in Russian nor in foreign publications and is currently exclusive. Objective – to determine the patterns of manifestation of contra- and ipsilateral effects, using individual changes in indicators of a simple auditory-motor response obtained as a result of longitudinal observations. The analysis of changes in the parameters of a simple auditory-motor reaction was carried out during mono-presentation of a sound signal on the left and on the right in 145 children and adolescents aged 7-14 years (470 measurements), who were under observation for 2 or more years, depending on changes in the mode of use mobile phone. Further, 48 cases were excluded (change in the lateralization of use, for example, the respondent brought the phone to both the right and the left ear, “changed” the leading ear, etc.). Three variants of changes in the mode of using MP in the longitude period (gradient of time change) were established: an increase in time (the group "less–more"), a decrease in time (group "more–less") and no changes. For each group, 4 variants of changes in the parameters of SAMR were revealed in the dynamics of observations along the longitude: decrease in SAMR indicators on the left (left ear) and increase in the right (right ear); decrease in indicators on the left and increase on the right and synchronous decrease or increase in indicators on the right and left. An assessment of the strength of the correlation was carried out (a linear Pearson correlation coefficient was used and an assessment of the statistical significance was carried out): between changes in the gradient of changes in the time of MP usage and SAMR, as well as changes in the lateralization of effects (changes in the frequency of occurrence of ipsi- and contralateral effects). The multivariance of changes in SAMR indicators and lateralization of effects was revealed depending on the mode of MP use. The proposed approach to the analysis of changes in a simple auditory-motor reaction and daily time of using a mobile phone makes it possible to suggest some mechanisms of changes in the parameters of the auditory analyzer in response to the duration of exposure to electromagnetic radiation from a mobile phone. The presence of contralateral effects of changes in SAMR parameters can serve as an assessment of the “capture” of the brain structures of children and adolescents by MP electromagnetic radiation. The results obtained once again prove the level of negative impact on the auditory system of children and adolescents. The current situation requires an immediate solution by clear regulation of the use of cellular communications by the younger generation.

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