scholarly journals Presence of antibodies to Crimean Congo haemorrhagic fever virus in sheep in Tunisia, North Africa

2021 ◽  
Author(s):  
Médiha Khamassi Khbou ◽  
Rihab Romdhane ◽  
Faten Bouaicha Zaafouri ◽  
Mohsen Bouajila ◽  
Limam Sassi ◽  
...  
Keyword(s):  
North Africa ◽  
Fever Virus ◽  
Haemorrhagic Fever ◽  
Crimean Congo Haemorrhagic Fever

scholarly journals Next-generation sequencing of southern African Crimean-Congo haemorrhagic fever virus isolates reveals a high frequency of M segment reassortment

2014 ◽  
Vol 142 (9) ◽  
pp. 1952-1962 ◽  
Author(s):  
D. GOEDHALS ◽  
P. A. BESTER ◽  
J. T. PAWESKA ◽  
R. SWANEPOEL ◽  
F. J. BURT
Keyword(s):  
Next Generation Sequencing ◽  
High Frequency ◽  
Complete Genome ◽  
Fever Virus ◽  
Likelihood Method ◽  
Haemorrhagic Fever ◽  
Next Generation ◽  
Genome Sequences ◽  
Crimean Congo Haemorrhagic Fever ◽  
Generation Sequencing

SUMMARYCrimean Congo haemorrhagic fever virus (CCHFV) is a bunyavirus with a single-stranded RNA genome consisting of three segments (S, M, L), coding for the nucleocapsid protein, envelope glycoproteins and RNA polymerase, respectively. To date only five complete genome sequences are available from southern African isolates. Complete genome sequences were generated for 10 southern African CCHFV isolates using next-generation sequencing techniques. The maximum-likelihood method was used to generate tree topologies for 15 southern African plus 26 geographically distinct complete sequences from GenBank. M segment reassortment was identified in 10/15 southern African isolates by incongruencies in grouping compared to the S and L segments. These reassortant M segments cluster with isolates from Asia/Middle East, while the S and L segments cluster with strains from South/West Africa. The CCHFV M segment shows a high level of genetic diversity, while the S and L segments appear to co-evolve. The reason for the high frequency of M segment reassortment is not known. It has previously been suggested that M segment reassortment results in a virus with high fitness but a clear role in increased pathogenicity has yet to be shown.

Factors driving the circulation and possible expansion of Crimean-Congo haemorrhagic fever virus in the western Palearctic

2012 ◽  
Vol 114 (1) ◽  
pp. 278-286 ◽  
Author(s):  
A. Estrada-Peña ◽  
F. Ruiz-Fons ◽  
P. Acevedo ◽  
C. Gortazar ◽  
J. de la Fuente
Keyword(s):  
Fever Virus ◽  
Haemorrhagic Fever ◽  
Western Palearctic ◽  
Crimean Congo Haemorrhagic Fever

scholarly journals Absence of Crimean-Congo haemorrhagic fever virus in the tick Hyalomma aegyptium parasitizing the spur-thighed tortoise (Testudo graeca) in Tunisia

Parasite ◽  
2019 ◽  
Vol 26 ◽  
pp. 35 ◽  
Author(s):  
Wasfi Fares ◽  
Khalil Dachraoui ◽  
Chawki Najjar ◽  
Hend Younsi ◽  
Stephen Findlay-Wilson ◽  
...  
Keyword(s):  
Tick Species ◽  
Fever Virus ◽  
Haemorrhagic Fever ◽  
Northern Tunisia ◽  
Testudo Graeca ◽  
Infestation Intensity ◽  
Main Host ◽  
Crimean Congo Haemorrhagic Fever ◽  
High Degree ◽  
Free Ranging

Free-ranging spur-thighed tortoises Testudo graeca, captured in different habitat types of Northern Tunisia from March to April 2017, were examined for tick infestation: 134/147 (91%) were infested. The overall infestation intensity and abundance was 8.5 and 7.8, respectively. From these tortoises, 1174 ticks were collected, of which 10% (n = 120) taken from 18 randomly-selected tortoises were identified at the species level; the remaining ticks were examined for the presence of Crimean-Congo haemorrhagic fever virus (CCHFv) by real time RT-PCR. Only adult Hyalomma aegyptium were found, suggesting a high degree of host specificity to tortoises. No CCHFv was detected in ticks. Considering the absence of CCHFv in Hyalomma aegyptium infesting its main host, the spur-thighed tortoise, this tick species is unlikely to play a major role in the epidemiology of CCHF. Therefore, more studies are needed to investigate the circulation of this arbovirus between livestock and other tick species from North Africa.

Host-passage-induced phenotypic changes in Crimean-Congo haemorrhagic fever virus

1995 ◽  
Vol 146 (2) ◽  
pp. 131-140 ◽  
Author(s):  
J.P. Gonzalez ◽  
M.L. Wilson ◽  
J.R Cornet ◽  
J.L. Camicas
Keyword(s):  
Fever Virus ◽  
Haemorrhagic Fever ◽  
Phenotypic Changes ◽  
Crimean Congo Haemorrhagic Fever

Detection of IgG antibody against Crimean-Congo haemorrhagic fever virus using ELISA with recombinant nucleoprotein antigens from genetically diverse strains

2013 ◽  
Vol 142 (10) ◽  
pp. 2147-2154 ◽  
Author(s):  
A. RANGUNWALA ◽  
R. R. SAMUDZI ◽  
F. J. BURT
Keyword(s):  
Amino Acid ◽  
South African ◽  
Cross Reactivity ◽  
Molecular Techniques ◽  
Fever Virus ◽  
Amino Acid Difference ◽  
Haemorrhagic Fever ◽  
Igg Antibody ◽  
Recombinant Antigens ◽  
Crimean Congo Haemorrhagic Fever

SUMMARYCrimean-Congo haemorrhagic fever virus (CCHFV) has the propensity to cause nosocomial infections with a high fatality rate. Handling the virus requires biosafety level-4 facilities, limiting accessibility for many laboratories. Advances in molecular techniques have allowed preparation of safe recombinant antigens that have application in diagnosis and serosurveillance of CCHFV. The aim of this study was to determine genetic diversity in CCHFV based on all available complete sequence data for the S gene encoding CCHFV nucleoprotein (NP) and antibody cross-reactivity between the NP of a South African isolate and the NP of a Greek isolate (AP92), the most genetically diverse CCHFV strain. The nucleotide sequence diversity and amino-acid diversity between genotypes, within genotypes and the pairwise distances were calculated for a dataset of 45 CCHFV isolates retrieved from GenBank. The most diverse virus, AP92, isolated from a tick in Greece, displayed the highest amino-acid difference (8·7%) with SPU415/85, isolated from a human infection in South Africa. Recombinant NP encoded for by codon-optimized S genes of SPU415/85 and AP92 were expressed in a bacterial host system and used to develop an in-house ELISA to detect IgG antibody against CCHFV in South African patients who survived infection. A total of 14/14 sera reacted with the South African recombinant NP and 13/14 reacted with the Greek recombinant NP. The serological cross-reactivity of the two NP antigens suggests that recombinant antigens prepared from geographically distinct CCHFV will have diagnostic and epidemiological applications worldwide.

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