Stable Expression of Mouse NADPH-Cytochrome P450 Reductase and Monkey P4501A1 cDNAs in Chinese Hamster Cells: Establishment of Cell Lines Highly Sensitive to Aflatoxin B1

1993 ◽  
Vol 300 (1) ◽  
pp. 164-168 ◽  
Author(s):  
M. Sawada ◽  
R. Kitamura ◽  
S. Ohgiya ◽  
T. Kamataki
Keyword(s):  
Cytochrome P450 ◽  
Cell Lines ◽  
Aflatoxin B1 ◽  
Chinese Hamster ◽  
Stable Expression ◽  
Cytochrome P450 Reductase ◽  
Chinese Hamster Cells ◽  
Nadph Cytochrome ◽  
Highly Sensitive ◽  
Nadph Cytochrome P450 Reductase

Bioactivation and Toxicity of Two Anthraquinones in V79 Chinese Hamster Cells

1991 ◽  
Vol 19 (1) ◽  
pp. 48-52
Author(s):  
Maurizio Mian ◽  
Duccio Fratta ◽  
Giuseppe Rainaldi ◽  
Dino Benetti ◽  
Pier Giovanni Gervasi
Keyword(s):  
Cytochrome P450 ◽  
Nadh Dehydrogenase ◽  
Chinese Hamster ◽  
Cytochrome P450 Reductase ◽  
V79 Cells ◽  
Chinese Hamster Cells ◽  
P450 Reductase ◽  
Nadph Cytochrome P450 Reductase ◽  
Dt Diaphorase

The role of the flavoprotein NADPH cytochrome P450 reductase, NADH-dehydrogenase and DT-diaphorase in the biotransformation of the anthraquinones rhein and danthron was investigated using purified enzymes, subcellular fractions and cell cultures. The results demonstrated that rhein was more able than danthron to produce O2 by P450 reductase, NADH-dehydrogenase and V79 homogenate and microsomes, and this may explain its higher toxicity to V79 cells. In addition, unlike other quinones, DT-diaphorase appeared not to play a detoxication role in rhein and danthron biotransformation. On the contrary, by inhibiting DT-diaphorase with dicoumarol, the rhein- and danthron-derived cytotoxicity in V79 cells was reduced. This finding suggests that, for these quinones, other coumarol-sensitive biotransformation routes are operative in V79 cells.

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