Stringency of Substrate-Specificity of Escherichia coli Malate-Dehydrogenase

1995 ◽  
Vol 322 (1) ◽  
pp. 43-52 ◽  
Author(s):  
W.E. Boernke ◽  
C.S. Millard ◽  
P.W. Stevens ◽  
S.N. Kakar ◽  
F.J. Stevens ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Substrate Specificity ◽  
Malate Dehydrogenase

The importance of arginine 102 for the substrate specificity of Escherichia coli malate dehydrogenase

1992 ◽  
Vol 189 (2) ◽  
pp. 1057-1062 ◽  
Author(s):  
David J. Nicholls ◽  
Julie Miller ◽  
Michael D. Scawen ◽  
Anthony R. Clarke ◽  
J. John Holbrook ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Substrate Specificity ◽  
Malate Dehydrogenase

Synthesis of γ-L-Glutamylalkylamides Catalyzed by Escherichia coli Having γ-Glutamyltranspeptidase Activity

2012 ◽  
Vol 550-553 ◽  
pp. 1177-1181
Author(s):  
Fei Zhang ◽  
Yin Liu ◽  
Pei Xin He
Keyword(s):  
Escherichia Coli ◽  
Substrate Specificity ◽  
Conversion Rate ◽  
Medicinal Value

γ-L-glutamyl alkylamines have potential medicinal value. In this research, five different γ-L-glutamyl alkylamines were synthesized. The result showed that substrate specificity of alkylamines, from high to low, is methylamine, ethylamine, n-propylamine, isopropylamine and n-butylamine. The reaction was optimal at pH 9.5 and 45°C, and the optimal substrate mole ratio of L-glutamine to alkylamine was 1:3 (mol/mol).Under these conditions, conversion rate of L-glutamine is about 90% (mol/mol)．

Overexpression of the Thermus aquaticus B malate dehydrogenase-encoding gene in Escherichia coli

Gene ◽  
1992 ◽  
Vol 114 (1) ◽  
pp. 139-143 ◽  
Author(s):  
Richard M. Alldread ◽  
David J. Nicholls ◽  
Trichur K. Sundaram ◽  
Michael D. Scawen ◽  
Tony Atkinson
Keyword(s):  
Escherichia Coli ◽  
Malate Dehydrogenase ◽  
Thermus Aquaticus ◽  
Encoding Gene
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