Expression of Human Cytochrome P450 2B6 in Escherichia coli: Characterization of Catalytic Activity and Expression Levels in Human Liver

2000 ◽  
Vol 376 (1) ◽  
pp. 206-216 ◽  
Author(s):  
Imad H. Hanna ◽  
James R. Reed ◽  
F.Peter Guengerich ◽  
Paul F. Hollenberg
Keyword(s):  
Escherichia Coli ◽  
Cytochrome P450 ◽  
Catalytic Activity ◽  
Human Liver ◽  
Expression Levels ◽  
Cytochrome P450 2B6 ◽  
Human Cytochrome

Effect of Commonly Used Organic Solvents on the Kinetics of Cytochrome P450 2B6- and 2C8-Dependent Activity in Human Liver Microsomes

2007 ◽  
Vol 35 (11) ◽  
pp. 1990-1995 ◽  
Author(s):  
Ragini Vuppugalla ◽  
Shu-Ying Chang ◽  
Hongjian Zhang ◽  
Punit H. Marathe ◽  
David A. Rodrigues
Keyword(s):  
Cytochrome P450 ◽  
Organic Solvents ◽  
Human Liver ◽  
Liver Microsomes ◽  
Human Liver Microsomes ◽  
Cytochrome P450 2B6 ◽  
Dependent Activity ◽  
Kinetics Of

Characterization of Diuron N-Demethylation by Mammalian Hepatic Microsomes and cDNA-Expressed Human Cytochrome P450 Enzymes

2007 ◽  
Vol 35 (9) ◽  
pp. 1634-1641 ◽  
Author(s):  
Khaled Abass ◽  
Petri Reponen ◽  
Miia Turpeinen ◽  
Jorma Jalonen ◽  
Olavi Pelkonen
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 Enzymes ◽  
Hepatic Microsomes ◽  
Human Cytochrome

scholarly journals Characterization of P450 FcpC, the Enzyme Responsible for Bioconversion of Diosgenone to Isonuatigenone in Streptomyces virginiae IBL-14

2009 ◽  
Vol 75 (12) ◽  
pp. 4202-4205 ◽  
Author(s):  
Wei Wang ◽  
Feng-Qing Wang ◽  
Dong-Zhi Wei
Keyword(s):  
Escherichia Coli ◽  
Electron Transfer ◽  
Cytochrome P450 ◽  
Cytochrome P450 Monooxygenase ◽  
P450 Monooxygenase ◽  
Whole Cell ◽  
Electron Transfer Chain ◽  
Streptomyces Virginiae ◽  
Transfer Chain

ABSTRACT A new cytochrome P450 monooxygenase, FcpC, from Streptomyces virginiae IBL-14 has been identified. This enzyme is found to be responsible for the bioconversion of a pyrano-spiro steroid (diosgenone) to a rare nuatigenin-type spiro steroid (isonuatigenone), which is a novel C-25-hydroxylated diosgenone derivative. A whole-cell P450 system was developed for the production of isonuatigenone via the expression of the complete three-component electron transfer chain in an Escherichia coli strain.

scholarly journals Structural and Thermodynamic Basis of (+)-α-Pinene Binding to Human Cytochrome P450 2B6

2013 ◽  
Vol 135 (28) ◽  
pp. 10433-10440 ◽  
Author(s):  
P. Ross Wilderman ◽  
Manish B. Shah ◽  
Hyun-Hee Jang ◽  
C. David Stout ◽  
James R. Halpert
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 2B6 ◽  
Human Cytochrome ◽  
Thermodynamic Basis
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