Expression, Purification, and Physical Properties of Recombinant Flavocytochrome Fusion Proteins Containing Rat Cytochrome b5 Linked to NADPH-Cytochrome P450 Reductase by Different Membrane-Binding Segments

Andrei A Gilep; Oleg L Guryev; Sergey A Usanov; Ronald W Estabrook

doi:10.1006/abbi.2001.2371

Expression, Purification, and Physical Properties of Recombinant Flavocytochrome Fusion Proteins Containing Rat Cytochrome b5 Linked to NADPH-Cytochrome P450 Reductase by Different Membrane-Binding Segments

Archives of Biochemistry and Biophysics ◽

10.1006/abbi.2001.2371 ◽

2001 ◽

Vol 390 (2) ◽

pp. 222-234 ◽

Cited By ~ 11

Author(s):

Andrei A Gilep ◽

Oleg L Guryev ◽

Sergey A Usanov ◽

Ronald W Estabrook

Keyword(s):

Cytochrome P450 ◽

Physical Properties ◽

Fusion Proteins ◽

Cytochrome B5 ◽

Membrane Binding ◽

Cytochrome P450 Reductase ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Nadph Cytochrome P450 Reductase

Download Full-text

Reconstitution of the Enzymatic Activities of Cytochrome P450s Using Recombinant Flavocytochromes Containing Rat Cytochrome b5 Fused to NADPH–Cytochrome P450 Reductase with Various Membrane-Binding Segments

Archives of Biochemistry and Biophysics ◽

10.1006/abbi.2001.2372 ◽

2001 ◽

Vol 390 (2) ◽

pp. 215-221 ◽

Cited By ~ 15

Author(s):

Andrei A. Gilep ◽

Oleg L. Guryev ◽

Sergey A. Usanov ◽

Ronald W. Estabrook

Keyword(s):

Cytochrome P450 ◽

Cytochrome B5 ◽

Membrane Binding ◽

Cytochrome P450s ◽

Enzymatic Activities ◽

Cytochrome P450 Reductase ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Nadph Cytochrome P450 Reductase

Download Full-text

An Enzymatically Active Chimeric Protein Containing the Hydrophilic Form of NADPH-Cytochrome P450 Reductase Fused to the Membrane-Binding Domain of Cytochrome b5

Biochemical and Biophysical Research Communications ◽

10.1006/bbrc.2001.5075 ◽

2001 ◽

Vol 284 (4) ◽

pp. 937-941 ◽

Cited By ~ 19

Author(s):

Andrei A. Gilep ◽

Oleg L. Guryev ◽

Sergey A. Usanov ◽

Ronald W. Estabrook

Keyword(s):

Cytochrome P450 ◽

Cytochrome B5 ◽

Membrane Binding ◽

Chimeric Protein ◽

Binding Domain ◽

Cytochrome P450 Reductase ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Nadph Cytochrome P450 Reductase

Download Full-text

Reduction of cytochrome b5 by NADPH–cytochrome P450 reductase

Archives of Biochemistry and Biophysics ◽

10.1016/j.abb.2005.06.019 ◽

2005 ◽

Vol 440 (2) ◽

pp. 204-211 ◽

Cited By ~ 56

Author(s):

F. Peter Guengerich

Keyword(s):

Cytochrome P450 ◽

Cytochrome B5 ◽

Cytochrome P450 Reductase ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Nadph Cytochrome P450 Reductase

Download Full-text

Features of the local immobilization of NADPH-cytochrome P450 reductase and cytochrome b5 on a solid surface

Russian Journal of General Chemistry ◽

10.1134/s1070363210060046 ◽

2010 ◽

Vol 80 (6) ◽

pp. 1060-1063

Author(s):

I. V. Paribok ◽

G. K. Zhavnerko ◽

V. E. Agabekov ◽

A. V. Yantsevich ◽

S. A. Usanov

Keyword(s):

Cytochrome P450 ◽

Solid Surface ◽

Cytochrome B5 ◽

Cytochrome P450 Reductase ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Nadph Cytochrome P450 Reductase

Download Full-text

The Primate Adrenal Zona Reticularis is Defined by Expression of Cytochrome b5, 17 -hydroxylase/17,20-lyase Cytochrome P450 (P450c17) and NADPH-Cytochrome P450 Reductase (reductase) but not 3 -Hydroxysteroid Dehydrogenase/ 5-4 Isomerase (3 -HSD)

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/jc.84.9.3382 ◽

1999 ◽

Vol 84 (9) ◽

pp. 3382-3385 ◽

Cited By ~ 21

Author(s):

S. Mapes

Keyword(s):

Cytochrome P450 ◽

Cytochrome B5 ◽

Hydroxysteroid Dehydrogenase ◽

Cytochrome P450 Reductase ◽

Zona Reticularis ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Nadph Cytochrome P450 Reductase

Download Full-text

Cytochrome b5 and NADPH‐Cytochrome P450 Reductase Compete for a Binding Site on the Proximal Surface of Cytochrome P450 2B4

The FASEB Journal ◽

10.1096/fasebj.21.5.a670-d ◽

2007 ◽

Vol 21 (5) ◽

Author(s):

Lucy Waskell ◽

Haoming Zhang ◽

Sang‐Choul Im ◽

Reza Razeghifard

Keyword(s):

Cytochrome P450 ◽

Binding Site ◽

Cytochrome B5 ◽

Cytochrome P450 Reductase ◽

Proximal Surface ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Cytochrome P450 2B4 ◽

Nadph Cytochrome P450 Reductase

Download Full-text

Interactions of mammalian cytochrome P450, NADPH-cytochrome P450 reductase, and cytochrome b5 enzymes

Archives of Biochemistry and Biophysics ◽

10.1016/j.abb.2004.12.008 ◽

2005 ◽

Vol 435 (1) ◽

pp. 207-216 ◽

Cited By ~ 52

Author(s):

Tsutomu Shimada ◽

Raymond L. Mernaugh ◽

F. Peter Guengerich

Keyword(s):

Cytochrome P450 ◽

Cytochrome B5 ◽

Cytochrome P450 Reductase ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Nadph Cytochrome P450 Reductase

Download Full-text

Comparison of CYP3A4 and CYP3A5: The Effects of Cytochrome b5 and NADPH-cytochrome P450 Reductase on Testosterone Hydroxylation Activities

Drug Metabolism and Pharmacokinetics ◽

10.2133/dmpk.dmpk-12-sh-030 ◽

2012 ◽

Vol 27 (6) ◽

pp. 663-667 ◽

Cited By ~ 13

Author(s):

Su-Jun Lee ◽

Joyce A. Goldstein

Keyword(s):

Cytochrome P450 ◽

Cytochrome B5 ◽

Cytochrome P450 Reductase ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Nadph Cytochrome P450 Reductase ◽

Testosterone Hydroxylation

Download Full-text

Cytochrome P450 3A, NADPH cytochrome P450 reductase and cytochrome b5 in the upper airways in horse

Research in Veterinary Science ◽

10.1016/j.rvsc.2007.09.012 ◽

2008 ◽

Vol 85 (1) ◽

pp. 80-85 ◽

Cited By ~ 12

Author(s):

E. Tydén ◽

L. Olsén ◽

J. Tallkvist ◽

H. Tjälve ◽

P. Larsson

Keyword(s):

Cytochrome P450 ◽

Cytochrome B5 ◽

Cytochrome P450 Reductase ◽

Cytochrome P450 3A ◽

Upper Airways ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Nadph Cytochrome P450 Reductase

Download Full-text

Biphasic effects of the flavonoids quercetin and naringenin on the metabolic activation of 2-amino-3,5-dimethylimidazo[4,5-f]quinoline by Salmonella typhimurium TA1538 co-expressing human cytochrome P450 1A2, NADPH-cytochrome P450 reductase, and cytochrome b5

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis ◽

10.1016/j.mrfmmm.2003.08.002 ◽

2004 ◽

Vol 545 (1-2) ◽

pp. 37-47 ◽

Cited By ~ 21

Author(s):

I Kang

Keyword(s):

Cytochrome P450 ◽

Salmonella Typhimurium ◽

Cytochrome B5 ◽

Metabolic Activation ◽

Cytochrome P450 Reductase ◽

Cytochrome P450 1A2 ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Human Cytochrome ◽

Nadph Cytochrome P450 Reductase

Download Full-text