Estimation of Receptor–Ligand Interactions by the Use of a Two-Marker System in Affinity Capillary Electrophoresis

2000 ◽  
Vol 280 (2) ◽  
pp. 209-215 ◽  
Author(s):  
Erica Mito ◽  
Ying Zhang ◽  
Sally Esquivel ◽  
Frank A. Gomez
Keyword(s):  
Capillary Electrophoresis ◽  
Affinity Capillary Electrophoresis ◽  
Receptor Ligand ◽  
Marker System ◽  
Ligand Interactions

Affinity Capillary Electrophoresis to Examine Receptor–Ligand Interactions

2004 ◽  
pp. 153-168 ◽  
Author(s):  
Maryam Azad ◽  
John Kaddis ◽  
Valerie Villareal ◽  
Lili Hernandez ◽  
Catherine Silverio ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Affinity Capillary Electrophoresis ◽  
Receptor Ligand ◽  
Ligand Interactions

Using Affinity Capillary Electrophoresis To Determine Binding Stoichiometries of Protein-Ligand Interactions

Biochemistry ◽  
1994 ◽  
Vol 33 (35) ◽  
pp. 10616-10621 ◽  
Author(s):  
Yen-Ho Chu ◽  
Watson J. Lees ◽  
Adonis Stassinopoulos ◽  
Christopher T. Walsh
Keyword(s):  
Capillary Electrophoresis ◽  
Affinity Capillary Electrophoresis ◽  
Protein Ligand Interactions ◽  
Ligand Interactions

Study of deoxyribonucleic acid–ligand interactions by partial filling affinity capillary electrophoresis

2014 ◽  
Vol 1349 ◽  
pp. 116-121 ◽  
Author(s):  
Martin Růžička ◽  
Martina Čížková ◽  
Michael Jirásek ◽  
Filip Teplý ◽  
Dušan Koval ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Deoxyribonucleic Acid ◽  
Affinity Capillary Electrophoresis ◽  
Acid Ligand ◽  
Partial Filling ◽  
Ligand Interactions

Modulation of Platelet Activation by Native DNA – Initial Description of Platelet Receptor Type, Number and Discrimination for Native DNA

1981 ◽  
Vol 45 (03) ◽  
pp. 263-266 ◽  
Author(s):  
B A Fiedel ◽  
M E Frenzke
Keyword(s):  
Platelet Aggregation ◽  
Human Platelets ◽  
Scatchard Analysis ◽  
Type Number ◽  
Single Class ◽  
Receptor Ligand ◽  
Platelet Receptor ◽  
Platelet Binding ◽  
Ligand Interactions ◽  
Initial Description

SummaryNative DNA (dsDNA) induces the aggregation of isolated human platelets. Using isotopically labeled dsDNA (125I-dsDNA) and Scatchard analysis, a single class of platelet receptor was detected with a KD = 190 pM and numbering ~275/platelet. This receptor was discriminatory in that heat denatured dsDNA, poly A, poly C, poly C · I and poly C · poly I failed to substantially inhibit either the platelet binding of, or platelet aggregation induced by, dsDNA; by themselves, these polynucleotides were ineffective as platelet agonists. However, poly G, poly I and poly G · I effectively and competitively inhibited platelet binding of the radioligand, independently activated the platelet and when used at a sub-activating concentration decreased the extent of dsDNA stimulated platelet aggregation. These data depict a receptor on human platelets for dsDNA and perhaps certain additional polynucleotides and relate receptor-ligand interactions to a physiologic platelet function.

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