scholarly journals Inhibition of Self-incompatible Pollen in Papaver rhoeas Involves a Complex Series of Cellular Events

2000 ◽  
Vol 85 ◽  
pp. 197-202 ◽  
Author(s):  
N JORDAN
Keyword(s):  
Papaver Rhoeas ◽  
Complex Series ◽  
Cellular Events ◽  
Incompatible Pollen

scholarly journals Self-Incompatibility in Papaver rhoeas L.: inhibition of incompatible pollen tube growth is dependent on pollen gene expression

1990 ◽  
Vol 116 (2) ◽  
pp. 319-324 ◽  
Author(s):  
VERNONICA E. FRANKLIN-TONG ◽  
MICHAEL J. LAWRENCE ◽  
F. CHRISTOPHER H. FRANKLIN
Keyword(s):  
Gene Expression ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Papaver Rhoeas ◽  
Incompatible Pollen ◽  
Incompatible Pollen Tube

Identification of Residues in a Hydrophilic Loop of the Papaver rhoeas S Protein That Play a Crucial Role in Recognition of Incompatible Pollen

1998 ◽  
Vol 10 (10) ◽  
pp. 1723
Author(s):  
Katsuyuki Kakeda ◽  
Nicholas D. Jordan ◽  
Alex Conner ◽  
Jon P. Ride ◽  
Vernonica E. Franklin-Tong ◽  
...  
Keyword(s):  
Crucial Role ◽  
S Protein ◽  
Papaver Rhoeas ◽  
Incompatible Pollen ◽  
Hydrophilic Loop

scholarly journals Investigating mechanisms involved in the self–incompatibility response in Papaver rhoeas

2003 ◽  
Vol 358 (1434) ◽  
pp. 1033-1036 ◽  
Author(s):  
Steve Thomas ◽  
Kim Osman ◽  
Barend H. J. de Graaf ◽  
Galina Shevchenko ◽  
Mike Wheeler ◽  
...  
Keyword(s):  
Actin Cytoskeleton ◽  
Nuclear Dna ◽  
Male Gametophyte ◽  
Mitogen Activated Protein Kinase ◽  
Self Incompatibility ◽  
Papaver Rhoeas ◽  
Incompatible Pollen ◽  
Mitogen Activated Protein ◽  
Signalling Cascade

Sexual reproduction in flowering plants is controlled by recognition mechanisms involving the male gametophyte (the pollen) and the female sporophyte (the pistil). Self–incompatibility (SI) involves the recognition and rejection of self– or incompatible pollen by the pistil. In Papaver rhoeas , SI uses a Ca 2+ –based signalling cascade triggered by the S –protein, which is encoded by the stigmatic component of the S –locus. This results in the rapid inhibition of incompatible pollen tube growth. We have identified several targets of the SI signalling cascade, including protein kinases, the actin cytoskeleton and nuclear DNA. Here, we summarize progress made on currently funded projects in our laboratory investigating some of the components targeted by SI, comprising (i) the characterization of a pollen phosphoprotein (p26) that is rapidly phosphorylated upon an incompatible SI response; (ii) the identification and characterization of a pollen mitogen–activated protein kinase (p56), which exhibits enhanced activation during SI; (iii) characterizing components involved in the reorganization and depolymerization of the actin cytoskeleton during the SI response; and (iv) investigating whether the SI response involves a programmed cell death signalling cascade.

scholarly journals Autophagy is required for self-incompatible pollen rejection in two transgenic Arabidopsis thaliana accessions

2020 ◽  
Author(s):  
Stuart R. Macgregor ◽  
Hyun Kyung Lee ◽  
Hayley Nelles ◽  
Daniel C. Johnson ◽  
Tong Zhang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
The Self ◽  
Self Incompatibility ◽  
Arabidopsis Lyrata ◽  
Additional Layer ◽  
Cellular Events ◽  
Pollen Rejection ◽  
Incompatible Pollen ◽  
Transgenic Lines ◽  
Stigmatic Papillae

AbstractSuccessful reproduction in the Brassicaceae is mediated by a complex series of interactions between the pollen and the pistil, and some species have an additional layer of regulation with the self-incompatibility trait. While the initial activation of the self-incompatibility pathway by the pollen S-locus protein11/S-locus cysteine-rich peptide and the stigma S Receptor Kinase is well characterized, the downstream mechanisms causing self-pollen rejection are still not fully understood. In previous studies, we had detected the presence of autophagic bodies with self-incompatible pollinations in Arabidopsis lyrata and transgenic A. thaliana lines, but it was not known if autophagy was essential for self-pollen rejection. Here, we investigated the requirement of autophagy in this response by crossing mutations in the essential AUTOPHAGY7 (ATG7) gene into two different transgenic self-incompatible A. thaliana lines in the Col-0 and C24 accessions. By using these previously characterized transgenic lines that express A. lyrata and A. halleri self-incompatibility genes, we demonstrated that disrupting autophagy can weaken their self-incompatible responses in the stigma. When the atg7 mutation was present, an increased number of self-incompatible pollen were found to hydrate and form pollen tubes that successfully fertilized the self-incompatible pistils. Additionally, we confirmed the presence of GFP-ATG8a labelled autophagosomes in the stigmatic papillae following self-incompatible pollinations. Together, these findings support the requirement of autophagy in the self-incompatibility response and add to the growing understanding of the cellular events that take place in the stigma to reject self-pollen.One Sentence SummaryIn self-incompatible transgenic Arabidopsis thaliana lines, autophagy is an integral part of the cellular responses in the stigma to efficiently block fertilization by self-incompatible pollen.

The genetics of self-incompatibility in Papaver rhoeas

1975 ◽  
Vol 188 (1092) ◽  
pp. 275-285 ◽  
Keyword(s):  
The Other ◽  
Self Incompatibility ◽  
Papaver Rhoeas ◽  
Incompatible Pollen ◽  
Other Hand ◽  
Intense Fluorescence

Incompatible pollen in this homomorphic species is inhibited on the stigma which suggests that determination of the pollen reaction might be sporophytic. On the other hand, the genetical evidence so far obtained suggests that the reaction of the pollen is under gametophytic control. Furthermore, the pollen is binucleate and callose appears to be laid down inside the grains of incompatible pollen which exhibit a zone of intense fluorescence when viewed under the microscope. The implications of these findings are discussed.

scholarly journals Identification of Residues in a Hydrophilic Loop of the Papaver rhoeas S Protein That Play a Crucial Role in Recognition of Incompatible Pollen

1998 ◽  
Vol 10 (10) ◽  
pp. 1723-1731 ◽  
Author(s):  
Katsuyuki Kakeda ◽  
Nicholas D. Jordan ◽  
Alex Conner ◽  
Jon P. Ride ◽  
Vernonica E. Franklin-Tong ◽  
...  
Keyword(s):  
Crucial Role ◽  
S Protein ◽  
Papaver Rhoeas ◽  
Incompatible Pollen ◽  
Hydrophilic Loop

Interim report on the structures at Howe, Stromness, Orkney

1984 ◽  
Vol 11 (1) ◽  
pp. 61-73 ◽  
Author(s):  
S P Carter ◽  
D Haigh ◽  
N R J Neil ◽  
Beverley Smith
Keyword(s):  
Iron Age ◽  
Interim Report ◽  
Complex Series ◽  
Settlement Site ◽  
Two Phases ◽  
Iron Working

Summary Excavations at Howe revealed a complex series of settlements which spanned the whole of the Iron Age period and were preceded by two phases of Neolithic activity. A probable stalled cairn was succeeded by a Maes Howe type chambered tomb which was later followed by enclosed settlements of which only scant remains survived. These settlements were replaced by a roundhouse with earth-house, built into the ruins of the chambered tomb. The roundhouse was surrounded by a contemporary defended settlement. Rebuilding led to the development of a broch structure and village. Partial collapse of tower brought about changes in the settlement, andalthougk some houses were maintained as domestic structures, others were rebuilt as iron-working sheds. The construction of smaller buildings and a later Iron Age or Pictish extended farmstead into rubble collapse accompanied a decline in the size of the settlement. The abandonment of the farmstead marked the end of Howe as a settlement site.

Repopulating Landscapes: Using Offence Data to Recreate Landscapes of Incarceration and Labour at the Port Arthur Penal Station, 1830–1877

2019 ◽  
Vol 13 (1-2) ◽  
pp. 155-181 ◽  
Author(s):  
Richard Tuffin ◽  
Martin Gibbs
Keyword(s):  
Labour Force ◽  
Spatial History ◽  
Complex Series ◽  
Industrial Landscape ◽  
Archaeological Landscape

For over half-a-century (1803–54), the Australian colony of Van Diemen's Land (Tasmania), played a key part in Britain's globe-spanning unfree diaspora. Today, a rich built and archaeological landscape, augmented by an exhaustive and relatively intact documentary archive, stand as eloquent markers to this convict legacy. As historical archaeologists, we have spent countless hours querying the physical and documentary residues in a bid to understand how the penological, social and economic imperatives of Britain and the colony shaped the management of convict labour. In particular, our task has centred upon the recovery of individual narratives – of both gaoler and gaoled – from such residues, moving away from a traditional focus on the broader outlines of the convict system. This paper illustrates how spatial history methodological processes have been used to relocate individual historic lives back into the convict industrial landscape of the Tasman Peninsula (Tasmania). Focusing on the male-only penal station of Port Arthur (1830–77), we will illustrate how we have reunited the physicality of past spaces and places, with the lives and labours of those who created and navigated them. Simple methodologies have been used to achieve this, designed with onward applicability in mind. A complex series of documents, convict conduct records, have been mined for spatial markers, allowing events and people to be relocated back into space. Through these processes of linkage and visualisation, we have been encouraged to ask further questions about the management of the unfree labour force and how this came to create the landscape we see today.

Fractions of anthocyanins in petals of Centaurea cyanus L. and Papaver rhoeas L.

2008 ◽  
Vol 21 (1) ◽  
pp. 45-54
Author(s):  
Beata Stefańczyk ◽  
Anna Woźniak ◽  
Kamil Górski ◽  
Agnieszka Skalska-Kamińska ◽  
Magdalena Wójciak-Kosior
Keyword(s):  
Papaver Rhoeas ◽  
Centaurea Cyanus
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