Identification of Residues in a Hydrophilic Loop of the Papaver rhoeas S Protein That Play a Crucial Role in Recognition of Incompatible Pollen

1998 ◽  
Vol 10 (10) ◽  
pp. 1723
Author(s):  
Katsuyuki Kakeda ◽  
Nicholas D. Jordan ◽  
Alex Conner ◽  
Jon P. Ride ◽  
Vernonica E. Franklin-Tong ◽  
...  
Keyword(s):  
Crucial Role ◽  
S Protein ◽  
Papaver Rhoeas ◽  
Incompatible Pollen ◽  
Hydrophilic Loop

scholarly journals Identification of Residues in a Hydrophilic Loop of the Papaver rhoeas S Protein That Play a Crucial Role in Recognition of Incompatible Pollen

1998 ◽  
Vol 10 (10) ◽  
pp. 1723-1731 ◽  
Author(s):  
Katsuyuki Kakeda ◽  
Nicholas D. Jordan ◽  
Alex Conner ◽  
Jon P. Ride ◽  
Vernonica E. Franklin-Tong ◽  
...  
Keyword(s):  
Crucial Role ◽  
S Protein ◽  
Papaver Rhoeas ◽  
Incompatible Pollen ◽  
Hydrophilic Loop

scholarly journals Self-Incompatibility in Papaver rhoeas L.: inhibition of incompatible pollen tube growth is dependent on pollen gene expression

1990 ◽  
Vol 116 (2) ◽  
pp. 319-324 ◽  
Author(s):  
VERNONICA E. FRANKLIN-TONG ◽  
MICHAEL J. LAWRENCE ◽  
F. CHRISTOPHER H. FRANKLIN
Keyword(s):  
Gene Expression ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Papaver Rhoeas ◽  
Incompatible Pollen ◽  
Incompatible Pollen Tube

Selective inhibition of the growth of incompatible pollen tubes by S-protein in the Japanese pear

2001 ◽  
Vol 13 (4) ◽  
pp. 209-215 ◽  
Author(s):  
S. Hiratsuka ◽  
Shao-Ling Zhang ◽  
E. Nakagawa ◽  
Yoshitaka Kawai
Keyword(s):  
Selective Inhibition ◽  
Pollen Tubes ◽  
Japanese Pear ◽  
S Protein ◽  
Incompatible Pollen

scholarly journals Investigating mechanisms involved in the self–incompatibility response in Papaver rhoeas

2003 ◽  
Vol 358 (1434) ◽  
pp. 1033-1036 ◽  
Author(s):  
Steve Thomas ◽  
Kim Osman ◽  
Barend H. J. de Graaf ◽  
Galina Shevchenko ◽  
Mike Wheeler ◽  
...  
Keyword(s):  
Actin Cytoskeleton ◽  
Nuclear Dna ◽  
Male Gametophyte ◽  
Mitogen Activated Protein Kinase ◽  
Self Incompatibility ◽  
Papaver Rhoeas ◽  
Incompatible Pollen ◽  
Mitogen Activated Protein ◽  
Signalling Cascade

Sexual reproduction in flowering plants is controlled by recognition mechanisms involving the male gametophyte (the pollen) and the female sporophyte (the pistil). Self–incompatibility (SI) involves the recognition and rejection of self– or incompatible pollen by the pistil. In Papaver rhoeas , SI uses a Ca 2+ –based signalling cascade triggered by the S –protein, which is encoded by the stigmatic component of the S –locus. This results in the rapid inhibition of incompatible pollen tube growth. We have identified several targets of the SI signalling cascade, including protein kinases, the actin cytoskeleton and nuclear DNA. Here, we summarize progress made on currently funded projects in our laboratory investigating some of the components targeted by SI, comprising (i) the characterization of a pollen phosphoprotein (p26) that is rapidly phosphorylated upon an incompatible SI response; (ii) the identification and characterization of a pollen mitogen–activated protein kinase (p56), which exhibits enhanced activation during SI; (iii) characterizing components involved in the reorganization and depolymerization of the actin cytoskeleton during the SI response; and (iv) investigating whether the SI response involves a programmed cell death signalling cascade.

scholarly journals Endothelial glycocalyx shields the interaction of SARS-CoV-2 spike protein with ACE2 receptors

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marta Targosz-Korecka ◽  
Agata Kubisiak ◽  
Damian Kloska ◽  
Aleksandra Kopacz ◽  
Anna Grochot-Przeczek ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Crucial Role ◽  
Protein S ◽  
Spike Protein ◽  
Endothelial Glycocalyx ◽  
S Protein ◽  
Human Pulmonary Artery ◽  
Contradictory Data ◽  
Adhesive Forces

AbstractEndothelial cells (ECs) play a crucial role in the development and propagation of the severe COVID-19 stage as well as multiorgan dysfunction. It remains, however, controversial whether COVID-19-induced endothelial injury is caused directly by the infection of ECs with SARS-CoV-2 or via indirect mechanisms. One of the major concerns is raised by the contradictory data supporting or denying the presence of ACE2, the SARS-CoV-2 binding receptor, on the EC surface. Here, we show that primary human pulmonary artery ECs possess ACE2 capable of interaction with the viral Spike protein (S-protein) and demonstrate the crucial role of the endothelial glycocalyx in the regulation of the S-protein binding to ACE2 on ECs. Using force spectroscopy method, we directly measured ACE2- and glycocalyx-dependent adhesive forces between S-protein and ECs and characterized the nanomechanical parameters of the cells exposed to S-protein. We revealed that the intact glycocalyx strongly binds S-protein but screens its interaction with ACE2. Reduction of glycocalyx layer exposes ACE2 receptors and promotes their interaction with S-protein. These results indicate that the susceptibility of ECs to COVID-19 infection may depend on the glycocalyx condition.

S-protein mutants indicate a functional role for SBP in the self-incompatibility reaction of Papaver rhoeas

1999 ◽  
Vol 20 (1) ◽  
pp. 119-125 ◽  
Author(s):  
Nicholas D. Jordan ◽  
Katsuyuki Kakeda ◽  
Alex Conner ◽  
Jon P. Ride ◽  
Vernonica E. Franklin-Tong ◽  
...  
Keyword(s):  
Functional Role ◽  
The Self ◽  
Self Incompatibility ◽  
S Protein ◽  
Papaver Rhoeas ◽  
Protein Mutants ◽  
Incompatibility Reaction

The genetics of self-incompatibility in Papaver rhoeas

1975 ◽  
Vol 188 (1092) ◽  
pp. 275-285 ◽  
Keyword(s):  
The Other ◽  
Self Incompatibility ◽  
Papaver Rhoeas ◽  
Incompatible Pollen ◽  
Other Hand ◽  
Intense Fluorescence

Incompatible pollen in this homomorphic species is inhibited on the stigma which suggests that determination of the pollen reaction might be sporophytic. On the other hand, the genetical evidence so far obtained suggests that the reaction of the pollen is under gametophytic control. Furthermore, the pollen is binucleate and callose appears to be laid down inside the grains of incompatible pollen which exhibit a zone of intense fluorescence when viewed under the microscope. The implications of these findings are discussed.

scholarly journals Recombinant stigmatic self-incompatibility (S-) protein elicits a Ca2+ transient in pollen of Papaver rhoeas

1995 ◽  
Vol 8 (2) ◽  
pp. 299-307 ◽  
Author(s):  
Vernonica E. Franklin-Tong ◽  
Jon P. Ride ◽  
F. Christopher H. Franklin
Keyword(s):  
Self Incompatibility ◽  
S Protein ◽  
Papaver Rhoeas

The Crucial Role of Social Support

2013 ◽  
Author(s):  
Norah C. Feeny
Keyword(s):  
Social Support ◽  
Crucial Role
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