Characterization of Bax-ς, a Cell Death-Inducing Isoform of Bax

2000 ◽  
Vol 270 (3) ◽  
pp. 868-879 ◽  
Author(s):  
Estelle Schmitt ◽  
Claudie Paquet ◽  
Myriam Beauchemin ◽  
Jessica Dever-Bertrand ◽  
Richard Bertrand
Keyword(s):  
Cell Death ◽  
A Cell

scholarly journals Identification and characterization of GRIM-1, a cell-death-associated gene product

2010 ◽  
Vol 123 (16) ◽  
pp. 2781-2791 ◽  
Author(s):  
E. R. Hofmann ◽  
S. C. Nallar ◽  
L. Lin ◽  
J. D'Cunha ◽  
D. J. Lindner ◽  
...  
Keyword(s):  
Cell Death ◽  
Gene Product ◽  
A Cell ◽  
Identification And Characterization

scholarly journals Biochemical Characterization of a Novel Redox-Regulated Metacaspase in a Marine Diatom

2021 ◽  
Vol 12 ◽  
Author(s):  
Shiri Graff van Creveld ◽  
Shifra Ben-Dor ◽  
Avia Mizrachi ◽  
Uria Alcolombri ◽  
Amanda Hopes ◽  
...  
Keyword(s):  
Cell Death ◽  
Biochemical Characterization ◽  
Site Directed Mutagenesis ◽  
Marine Diatom ◽  
Type Iii ◽  
Calcium Dependent ◽  
Evolutionary Origins ◽  
Cell Extracts ◽  
A Cell

Programmed cell death (PCD) in marine microalgae was suggested to be one of the mechanisms that facilitates bloom demise, yet its molecular components in phytoplankton are unknown. Phytoplankton are completely lacking any of the canonical components of PCD, such as caspases, but possess metacaspases. Metacaspases were shown to regulate PCD in plants and some protists, but their roles in algae and other organisms are still elusive. Here, we identified and biochemically characterized a type III metacaspase from the model diatom Phaeodactylum tricornutum, termed PtMCA-IIIc. Through expression of recombinant PtMCA-IIIc in E. coli, we revealed that PtMCA-IIIc exhibits a calcium-dependent protease activity, including auto-processing and cleavage after arginine. Similar metacaspase activity was detected in P. tricornutum cell extracts. PtMCA-IIIc overexpressing cells exhibited higher metacaspase activity, while CRISPR/Cas9-mediated knockout cells had decreased metacaspase activity compared to WT cells. Site-directed mutagenesis of cysteines that were predicted to form a disulfide bond decreased recombinant PtMCA-IIIc activity, suggesting its enhancement under oxidizing conditions. One of those cysteines was oxidized, detected in redox proteomics, specifically in response to lethal concentrations of hydrogen peroxide and a diatom derived aldehyde. Phylogenetic analysis revealed that this cysteine-pair is unique and widespread among diatom type III metacaspases. The characterization of a cell death associated protein in diatoms provides insights into the evolutionary origins of PCD and its ecological significance in algal bloom dynamics.

scholarly journals MORPHOLOGICAL AND BIOCHEMICAL CHARACTERIZATION OF A CELL DEATH PROCESS INDUCED BY E3, A NEW SYNTHETIC DIARYLSULFONILUREA ANALOGUE

2001 ◽  
Vol 1 (S3) ◽  
pp. 50-50
Author(s):  
M. Alonso ◽  
M. Miglaccio ◽  
I. Encio ◽  
A. Asumendi ◽  
V. Martinez-Merino ◽  
...  
Keyword(s):  
Cell Death ◽  
Biochemical Characterization ◽  
Death Process ◽  
Cell Death Process ◽  
A Cell

scholarly journals Creation and characterization of a cell-death reporter cell line for hepatitis C virus infection

2010 ◽  
Vol 86 (2) ◽  
pp. 220-223 ◽  
Author(s):  
Zhilei Chen ◽  
Rudo L. Simeon ◽  
Karuppiah Chockalingam ◽  
Charles M. Rice
Keyword(s):  
Cell Death ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Cell Line ◽  
Reporter Cell Line ◽  
Hepatitis C Virus Infection ◽  
Reporter Cell ◽  
A Cell

scholarly journals Retraction: Identification and characterization of GRIM-1, a cell-death-associated gene product

2021 ◽  
Vol 134 (22) ◽  
Author(s):  
Edward R. Hofmann ◽  
Shreeram C. Nallar ◽  
Limei Lin ◽  
Jonathan D'Cunha ◽  
Daniel J. Lindner ◽  
...  
Keyword(s):  
Cell Death ◽  
Gene Product ◽  
A Cell ◽  
Identification And Characterization

Characterization of a cell death‐inducing endonuclease‐like venom protein from the parasitoid wasp Pteromalus puparum ( Hymenoptera: Pteromalidae )

2020 ◽  
Vol 77 (1) ◽  
pp. 224-233
Author(s):  
Jiale Wang ◽  
Zhichao Yan ◽  
Shan Xiao ◽  
Beibei Wang ◽  
Qi Fang ◽  
...  
Keyword(s):  
Cell Death ◽  
Parasitoid Wasp ◽  
Venom Protein ◽  
Pteromalus Puparum ◽  
A Cell

scholarly journals A redox-regulated type III metacaspase controls cell death in a marine diatom

2018 ◽  
Author(s):  
Shiri Graff van Creveld ◽  
Shifra Ben-Dor ◽  
Avia Mizrachi ◽  
Uria Alcolombri ◽  
Amanda Hopes ◽  
...  
Keyword(s):  
Cell Death ◽  
Large Scale ◽  
Redox Regulation ◽  
Marine Phytoplankton ◽  
Marine Diatom ◽  
Type Iii ◽  
Calcium Dependent ◽  
A Cell ◽  
Regulate Cell Death

Programmed cell death (PCD) in marine phytoplankton was suggested as one of the mechanisms that facilitates large scale bloom demise. Yet, the molecular basis for algal PCD machinery is rudimentary. Metacaspases are considered ancestral proteases that regulate cell death, but their activity and role in algae are still elusive. Here we biochemically characterized a recombinant metacaspase 5 from the model diatom Phaeodactylum tricornutum (PtMC5), revealing calcium-dependent protease activity. This activity includes auto-processing and cleavage following arginine. PtMC5 overexpressing cells exhibited higher metacaspase activity and were more sensitive to a diatom-specific infochemical compared to WT cells. Mutagenesis of potential disulfide-forming cysteines decreased PtMC5 activity, suggesting redox regulation. This cysteine pair is widespread in diatom type III metacaspases, but was not found in any other taxa. The characterization of a cell death associated protein in marine phytoplankton will enable deeper understanding of the ecological significance of PCD in bloom dynamics.

scholarly journals Morphological and Biochemical Characterization of a Cell Death Process Induced by E3, a New Synthetic Diarylsulfonilurea Analogue

2001 ◽  
Vol 1 ◽  
pp. 50-50
Author(s):  
M. Alonso ◽  
M. Miglaccio ◽  
I. Encío ◽  
A. Asumendi ◽  
V. Martinez-Merino ◽  
...  
Keyword(s):  
Cell Death ◽  
Biochemical Characterization ◽  
Death Process ◽  
Cell Death Process ◽  
A Cell

Characterization of a cell wall beta-galactosidase of Cicer arietinum epicotyls involved in cell wall autolysis

1990 ◽  
Vol 80 (4) ◽  
pp. 629-635 ◽  
Author(s):  
Berta Dopico ◽  
Gregorio Nicolas ◽  
Emilia Labrador
Keyword(s):  
Cell Wall ◽  
Cicer Arietinum ◽  
A Cell ◽  
Beta Galactosidase

A Cell Death Pathway Induced by Antibody-Mediated Cross-Linking of CD45 on Lymphocytes

2003 ◽  
Vol 23 (5-6) ◽  
pp. 421-440 ◽  
Author(s):  
Ann-Muriel Steff ◽  
Marylene Fortin ◽  
Fabianne Philippoussis ◽  
Sylvie Lesage ◽  
Chantal Arguin ◽  
...  
Keyword(s):  
Cell Death ◽  
Cross Linking ◽  
Cell Death Pathway ◽  
A Cell
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