EXPRESSION OF THE CELL ADHESION MOLECULE E-CADHERIN BY THE HUMAN BONE MARROW STROMAL CELLS AND ITS PROBABLE ROLE IN CD34+STEM CELL ADHESION

1998 ◽  
Vol 22 (9-10) ◽  
pp. 641-648 ◽  
Author(s):  
K TUREL
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Cell Adhesion Molecule ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Probable Role

scholarly journals Vascular cell adhesion molecule-1 expressed by bone marrow stromal cells mediates the binding of hematopoietic progenitor cells

Blood ◽  
1992 ◽  
Vol 80 (2) ◽  
pp. 388-395 ◽  
Author(s):  
PJ Simmons ◽  
B Masinovsky ◽  
BM Longenecker ◽  
R Berenson ◽  
B Torok-Storb ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Stromal Cells ◽  
Cell Adhesion Molecule ◽  
Marrow Stromal Cells ◽  
Vascular Cell Adhesion ◽  
Vascular Cell ◽  
Cd34 Cells ◽  
Cell Adhesion Molecule 1

Human bone marrow-derived CD34+ cells were analyzed for the expression of the beta 1-family of integrin adhesion molecules. Integrin alpha 4 beta 1 was consistently expressed by greater than 90% of CD34+ cells, including essentially all assayable granulocyte-macrophage colony- forming cells (CFU-GM) and erythroid bursts (BFU-E) as shown by fluorescence-activated cell sorting studies. Adhesion of highly enriched CD34+ cells to cultured allogeneic marrow stromal cells was largely inhibited both by monoclonal antibody to alpha 4 beta 1 and to vascular cell adhesion molecule-1 (VCAM-1), a ligand for alpha 4 beta 1. VCAM-1 was found to be expressed by bone marrow stromal elements in vitro both constitutively at low level and at high levels after treatment with cytokines. Induction of VCAM-1 was cytokine- and time- dependent with maximum levels being obtained after 4 hours of exposure to a combination of interleukin-4 and tumor necrosis factor-alpha. Cytokine-induced stromal cells bound threefold higher numbers of CFU-GM and BFU-E, this increase being abrogated by anti-alpha 4 beta 1 and anti-VCAM-1 antibodies. In addition, the adhesion to stroma of more immature progenitors, the long-term culture initiating cells, also occurred through an alpha 4 beta 1/VCAM-1-dependent mechanism. These studies identify an adhesion mechanism of potential importance in the localization of primitive progenitors within the hematopoietic microenvironment.

scholarly journals Vascular cell adhesion molecule-1 expressed by bone marrow stromal cells mediates the binding of hematopoietic progenitor cells

Blood ◽  
1992 ◽  
Vol 80 (2) ◽  
pp. 388-395 ◽  
Author(s):  
PJ Simmons ◽  
B Masinovsky ◽  
BM Longenecker ◽  
R Berenson ◽  
B Torok-Storb ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Stromal Cells ◽  
Cell Adhesion Molecule ◽  
Marrow Stromal Cells ◽  
Vascular Cell Adhesion ◽  
Vascular Cell ◽  
Cd34 Cells ◽  
Cell Adhesion Molecule 1

Abstract Human bone marrow-derived CD34+ cells were analyzed for the expression of the beta 1-family of integrin adhesion molecules. Integrin alpha 4 beta 1 was consistently expressed by greater than 90% of CD34+ cells, including essentially all assayable granulocyte-macrophage colony- forming cells (CFU-GM) and erythroid bursts (BFU-E) as shown by fluorescence-activated cell sorting studies. Adhesion of highly enriched CD34+ cells to cultured allogeneic marrow stromal cells was largely inhibited both by monoclonal antibody to alpha 4 beta 1 and to vascular cell adhesion molecule-1 (VCAM-1), a ligand for alpha 4 beta 1. VCAM-1 was found to be expressed by bone marrow stromal elements in vitro both constitutively at low level and at high levels after treatment with cytokines. Induction of VCAM-1 was cytokine- and time- dependent with maximum levels being obtained after 4 hours of exposure to a combination of interleukin-4 and tumor necrosis factor-alpha. Cytokine-induced stromal cells bound threefold higher numbers of CFU-GM and BFU-E, this increase being abrogated by anti-alpha 4 beta 1 and anti-VCAM-1 antibodies. In addition, the adhesion to stroma of more immature progenitors, the long-term culture initiating cells, also occurred through an alpha 4 beta 1/VCAM-1-dependent mechanism. These studies identify an adhesion mechanism of potential importance in the localization of primitive progenitors within the hematopoietic microenvironment.

scholarly journals Mesenchymal Stem Cells in Perichondrium Express Activated Leukocyte Cell Adhesion Molecule and Participate in Bone Marrow Formation

2002 ◽  
Vol 195 (12) ◽  
pp. 1549-1563 ◽  
Author(s):  
Fumio Arai ◽  
Osamu Ohneda ◽  
Takeshi Miyamoto ◽  
Xiu Qin Zhang ◽  
Toshio Suda
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Cell Adhesion Molecule ◽  
Hematopoietic Bone Marrow

Perichondrium in fetal limb is composed of undifferentiated mesenchymal cells. However, the multipotency of cells in this region and the role of perichondrium in bone marrow formation are not well understood. In this report, we purified and characterized perichondrial cells using a monoclonal antibody against activated leukocyte cell adhesion molecule (ALCAM) and investigated the role of perichondrial cells in hematopoietic bone marrow formation. ALCAM is expressed on hematopoietic cells, endothelial cells, bone marrow stromal cells, and mesenchymal stem cells and mediates homophilic (ALCAM–ALCAM)/heterophilic (ALCAM-CD6) cell adhesion. Here we show by immunohistochemical staining that ALCAM is expressed in perichondrium. ALCAM+ perichondrial cells isolated by FACS® exhibit the characteristics of mesenchymal stem cells. ALCAM+ cells can differentiate into osteoblasts, adipocytes, chondrocytes, and stromal cells, which can support osteoclastogenesis, hematopoiesis, and angiogenesis. Furthermore, the addition of ALCAM-Fc or CD6-Fc to the metatarsal culture, the invasion of the blood vessels to a cartilage was inhibited. Our findings indicate that ALCAM+ perichondrial cells participate in vascular invasion by recruiting osteoclasts and vessels. These findings suggest that perichondrium might serve as a stem cell reservoir and play an important role in the early development of a bone and bone marrow.

Clonal mesenchymal progenitors from human bone marrow differentiate in vitro according to a hierarchical model

2000 ◽  
Vol 113 (7) ◽  
pp. 1161-1166 ◽  
Author(s):  
A. Muraglia ◽  
R. Cancedda ◽  
R. Quarto
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Differentiation Potential ◽  
Mesenchymal Progenitors

Bone marrow stromal cells can give rise to several mesenchymal lineages. The existence of a common stem/progenitor cell, the mesenchymal stem cell, has been proposed, but which developmental stages follow this mesenchymal multipotent progenitor is not known. Based on experimental evidence, a model of mesenchymal stem cell differentiation has been proposed in which individual lineages branch directly from the same progenitor. We have verified this model by using clonal cultures of bone marrow derived stromal fibroblasts. We have analyzed the ability of 185 non-immortalized human bone marrow stromal cell clones to differentiate into the three main lineages: osteo-, chondro- and adipogenic. All clones but one differentiated into the osteogenic lineage. About one third of the clones differentiated into all three lineages analyzed. Most clones (60-80%) displayed an osteo-chondrogenic potential. We have never observed clones with a differentiation potential limited to the osteo-adipo- or to the chondro-adipogenic phenotype, nor pure chondrogenic and adipogenic clones. How long the differentiation potential of a number of clones was maintained was assessed throughout their life span. Clones progressively lost their adipogenic and chondrogenic differentiation potential at increasing cell doublings. Our data suggest a possible model of predetermined bone marrow stromal cells differentiation where the tripotent cells can be considered as early mesenchymal progenitors that display a sequential loss of lineage potentials, generating osteochondrogenic progenitors which, in turn, give rise to osteogenic precursors.

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