LOCALIZATION OF THE GLUT1 GLUCOSE TRANSPORTER TO BREFELDIN A-SENSITIVE VESICLES OF DIFFERENTIATED CIT3MOUSE MAMMARY EPITHELIAL CELLS

2001 ◽  
Vol 25 (4) ◽  
pp. 277-288 ◽  
Author(s):  
P Haney
Keyword(s):  
Epithelial Cells ◽  
Glucose Transporter ◽  
Mammary Epithelial Cells ◽  
Brefeldin A ◽  
Mammary Epithelial ◽  
Glut1 Glucose Transporter

Glucose transporter in bovine mammary epithelial cells is an asymmetric carrier that exhibits cooperativity and trans-stimulation

2003 ◽  
Vol 285 (5) ◽  
pp. C1226-C1234 ◽  
Author(s):  
Changting Xiao ◽  
John P. Cant
Keyword(s):  
Epithelial Cells ◽  
Glucose Transporter ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Isolated Cells ◽  
Experimental Conditions ◽  
Bovine Mammary Epithelial Cells ◽  
Equilibrium Exchange ◽  
Uptake Activity

Glucose transport kinetics were quantified in isolated bovine mammary epithelial cells using 3- O-methyl-d-glucose. Isolated cells retained satisfactory viability and glucose uptake activity, which was inhibited by cytochalasin B, phloretin, HgCl2, and low temperature. Initial rates of entry were measured over a 15-s interval at 37°C under zero- trans, equilibrium-exchange, high- cis, and high- trans concentrations of 3- O-methyl-d-glucose between 0 and 20 mM. The combined set of rate measurements from all experimental conditions was fit to the fixed-site carrier model by nonlinear regression to estimate parameters of transport. For the regression between predicted and observed initial rates, r2 was 0.97. Forward Vmax was estimated at 18.2 nmol·min-1·mg protein-1, and the Michaelis constant was 8.29 mM. The cooperativity parameter was 1.63, trans-stimulation was 2.13-fold, and asymmetry was 2.06-fold. On the basis of the kinetic parameters, variations in intracellular glucose concentrations are not responsible for the range of glucose uptakes by bovine mammary glands observed in vivo.

Expression and localisation of glucose transporter 1 (GLUT1) in dairy goat mammary gland at different physiological stages

2009 ◽  
Vol 89 (4) ◽  
pp. 475-480 ◽  
Author(s):  
Z Na ◽  
L Qingzhang ◽  
G Xuejun ◽  
N Xuemei ◽  
Y Hongbo ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Epithelial Cells ◽  
Glucose Transporter ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Dairy Goat ◽  
Apical Plasma Membrane ◽  
Glucose Transporter 1 ◽  
Glut1 Protein ◽  
Glut1 Mrna

Glucose is the major energy source for mammary epithelial cells, as well as an important substrate for lactose synthesis. Mammary epithelial cells take up glucose from extracellular fluid into the cell through glucose transporter (GLUT). This study was aimed at investigating the expression of GLUT1 glucose transporter in dairy goat mammary gland during puberty, pregnancy, lactation, and involution. Using real-time reverse transcription PCR (qRT-PCR) and Western blotting, we analyzed the expression of GLUT1 mRNA and protein in dairy goat mammary gland. GLUT1 mRNA and protein expression increased during pregnancy and lactation, especially at peak lactation, and decreased strongly after weaning. Furthermore, the location of GLUT1 protein was determined by immunofluorescence laser confocal microscopy. GLUT1 protein localised to the basal and apical plasma membrane of epithelial cells, and also in the cytoplasm. The results from this study showed that GLUT1 is expressed in the dairy goat mammary gland with the greatest expression found in mammary epithelial cells during pregnancy and lactation.Key words: Expression, glucose transporter, goat, mammary gland

Peroxisome proliferator-activated receptor β/δ does not regulate glucose uptake and lactose synthesis in bovine mammary epithelial cells cultivated in vitro

2018 ◽  
Vol 85 (3) ◽  
pp. 295-302 ◽  
Author(s):  
Jayant Lohakare ◽  
Johan S Osorio ◽  
Massimo Bionaz
Keyword(s):  
Epithelial Cells ◽  
Glucose Uptake ◽  
Glucose Transporter ◽  
Mammary Epithelial Cells ◽  
Significant Inhibition ◽  
Mammary Epithelial ◽  
Pyruvate Dehydrogenase Kinase ◽  
Bovine Mammary Epithelial Cells ◽  
Gene Reporter Assay ◽  
Lactose Synthesis

The hypothesis of the study was that inhibition of PPARβ/δ increases glucose uptake and lactose synthesis in bovine mammary epithelial cells by reducing the expression of the glucose transporter mRNA destabiliser calreticulin. Three experiments were conducted to test the hypothesis using immortalised bovine mammary alveolar (MACT) and primary bovine mammary (PBMC) cells. In Experiment 1, the most effective dose to inhibit PPARβ/δ activity among two synthetic antagonists (GSK-3787 and PT-s58) was assessed using a gene reporter assay. In Experiment 2, the effect on glucose uptake and lactose synthesis was evaluated by measuring glucose and lactose in the media and expression of related key genes upon modulation of PPARβ/δ using GSK-3787, the synthetic PPARβ/δ agonist GW-501516, or a combination of the two in cells cultivated in plastic. In Experiment 3, the same treatments were applied to cells cultivated in Matrigel and glucose and lactose in media were measured. In Experiment 1 it was determined that a significant inhibition of PPARβ/δ in the presence or absence of fetal bovine serum was achieved with ≥ 1000 nm GSK-3787 but no significant inhibition was observed with PT-s58. In Experiment 2, inhibition of PPARβ/δ had no effect on glucose uptake and lactose synthesis but they were both increased by GW-501516 in PBMC. The mRNA abundance of PPARβ/δ target gene pyruvate dehydrogenase kinase 4 was increased but transcription of calreticulin was decreased (only in MACT cells) by GW-501516. Treatment with GSK-3787 did not affect the transcription of measured genes. No effects on glucose uptake or lactose synthesis were detected by modulation of PPARβ/δ activity on cells cultivated in Matrigel. The above data do not provide support for the original hypothesis and suggest that PPARβ/δ does not play a major role in glucose uptake and lactose synthesis in bovine mammary epithelial cells.

Human mammary epithelial cells are sensitized to ionizing radiation by knockdown of caveolin-1

2010 ◽  
Vol 34 (8) ◽  
pp. S7-S7
Author(s):  
Nan Zhang ◽  
Jian Che ◽  
Zheng Wu ◽  
Yang Wang ◽  
Liwei Liu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Ionizing Radiation ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Caveolin 1 ◽  
Human Mammary Epithelial Cells ◽  
Human Mammary Epithelial

Microphysiometry to Evaluate Real-Time Response of Mammary Epithelial Cells to IGF-I

2000 ◽  
Vol 28 (1-2) ◽  
pp. 209-211 ◽  
Author(s):  
Rose Marie Robinson ◽  
R. Michael Akers ◽  
Kimberly E. Forsten
Keyword(s):  
Epithelial Cells ◽  
Real Time ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Time Response ◽  
Igf I
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