Prevention of Autoimmune Diabetes in Nonobese Diabetic Female Mice by Treatment with Recombinant Glutamic Acid Decarboxylase (GAD 65)

1995 ◽  
Vol 76 (1) ◽  
pp. 90-95 ◽  
Author(s):  
Jean-Marie Pleau ◽  
Flavia Fernandez-Saravia ◽  
Anne Esling ◽  
Françoise Homo-Delarche ◽  
Mireille Dardenne
Keyword(s):  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Autoimmune Diabetes ◽  
Acid Decarboxylase ◽  
Female Mice ◽  
Nonobese Diabetic ◽  
Gad 65

scholarly journals Normal Incidence of Diabetes in NOD Mice Tolerant to Glutamic Acid Decarboxylase

2003 ◽  
Vol 197 (12) ◽  
pp. 1635-1644 ◽  
Author(s):  
Elmar Jaeckel ◽  
Ludger Klein ◽  
Natalia Martin-Orozco ◽  
Harald von Boehmer
Keyword(s):  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Tolerance Induction ◽  
Nod Mice ◽  
Normal Incidence ◽  
Invariant Chain ◽  
Acid Decarboxylase ◽  
Β Cells ◽  
Nonobese Diabetic ◽  
Specific Tolerance

Experiments in nonobese diabetic (NOD) mice that lacked expression of glutamic acid decarboxylase (GAD) in β cells have suggested that GAD represents an autoantigen essential for initiating and maintaining the diabetogenic immune response. Several attempts of inducing GAD-specific recessive tolerance to support this hypothesis have failed. Here we report on successful tolerance induction by expressing a modified form of GAD under control of the invariant chain promoter resulting in efficient epitope display. In spite of specific tolerance insulitis and diabetes occurred with normal kinetics indicating that GAD is not an essential autoantigen in the pathogenesis of diabetes.

Peptide specificity of high-titer anti-glutamic acid decarboxylase (GAD)65 autoantibodies

1998 ◽  
Vol 62 (3) ◽  
pp. 123-130 ◽  
Author(s):  
Faı̈za Rharbaoui ◽  
Claude Granier ◽  
Mouna Kellou ◽  
Jean-Claude Mani ◽  
Peter van Endert ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
High Titer ◽  
Acid Decarboxylase ◽  
Peptide Specificity ◽  
Gad 65

scholarly journals Evaluation of Two Nonisotopic Immunoassays for Determination of Glutamic Acid Decarboxylase and Tyrosine Phosphatase Autoantibodies in Serum

2004 ◽  
Vol 50 (8) ◽  
pp. 1378-1382 ◽  
Author(s):  
Xavier Palomer ◽  
Dídac Mauricio ◽  
José Rodríguez-Espinosa ◽  
Edgar Zapico ◽  
Carme Mayoral ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Clinical Laboratory ◽  
Autoimmune Diabetes ◽  
Tyrosine Phosphatase ◽  
Acid Decarboxylase ◽  
Time Resolved ◽  
Radiobinding Assay

Abstract Background: Autoantibodies for the 65-kDa form of glutamic acid decarboxylase (GAD65) and protein tyrosine phosphatase-like protein (IA-2) are measured for risk prediction and diagnosis of autoimmune diabetes mellitus. There is a lack of adequate nonisotopic alternatives to the most widely used method for both autoantibodies, which is a radiobinding assay (RBA). Methods: We compared two commercially available immunoassays, an ELISA and a time-resolved immunofluorometric assay (TR-IFMA), with RBA. Results: We found excellent agreement between the RBA and ELISA for measurement of GAD65 autoantibodies (GADAs); they showed comparable analytical precision in the cutoff range and achieved similar diagnostic specificity. The ELISA identified more GADA-positive individuals among patients with new-onset type 1 diabetes than did the RBA [89% (95% confidence interval, 78–95%), vs 71% (58–82%); P <0.03]. For IA-2 autoantibodies (IA-2As), only the TR-IFMA achieved analytical performance and diagnostic accuracy comparable to that of the RBA. These results with the GADA ELISA and IA-2A TR-IFMA were consistent with those obtained blindly in the Diabetes Antibody Standardization Program 2003. The performance of the GADA TR-IFMA and IA-2A ELISA was unsatisfactory, and these tests were not subjected to clinical evaluation. Conclusions: The GADA ELISA and IA-2A TR-IFMA behave comparably with RBA and are thus suitable for use in the clinical laboratory.

Localization of Glutamic Acid Decarboxylase in the Kidneys of Nonobese Diabetic Mice

Nephron ◽  
1996 ◽  
Vol 72 (4) ◽  
pp. 662-666 ◽  
Author(s):  
Zhi-Hong Liu ◽  
Liliane J. Striker ◽  
M. Hattori ◽  
Chih-Wei Yang ◽  
Gary E. Striker
Keyword(s):  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Acid Decarboxylase ◽  
Diabetic Mice ◽  
Nonobese Diabetic ◽  
Nonobese Diabetic Mice

Immunotherapy of Autoimmune Diabetes by Nasal Administration of Tandem Glutamic Acid Decarboxylase 65 Peptides

2009 ◽  
Vol 38 (8) ◽  
pp. 690-703 ◽  
Author(s):  
Huaqian Wang ◽  
Jie Yang ◽  
Liang Jin ◽  
Jiao Feng ◽  
Yong Lu ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Autoimmune Diabetes ◽  
Nasal Administration ◽  
Acid Decarboxylase ◽  
Glutamic Acid Decarboxylase 65
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