Differential Regulation of Gap Junction Protein (Connexin) Genes during Cardiomyocytic Differentiation of Mouse Embryonic Stem Cellsin Vitro

1996 ◽  
Vol 229 (2) ◽  
pp. 318-326 ◽  
Author(s):  
Yumiko Oyamada ◽  
Kiyoshi Komatsu ◽  
Hisakazu Kimura ◽  
Michio Mori ◽  
Masahito Oyamada
Keyword(s):  
Gap Junction ◽  
Embryonic Stem ◽  
Differential Regulation ◽  
Junction Protein ◽  
Gap Junction Protein ◽  
Connexin Genes

scholarly journals Focal gap junction uncoupling and spontaneous ventricular ectopy

2005 ◽  
Vol 289 (3) ◽  
pp. H1091-H1098 ◽  
Author(s):  
David E. Gutstein ◽  
Stephan B. Danik ◽  
Steve Lewitton ◽  
David France ◽  
Fangyu Liu ◽  
...  
Keyword(s):  
Gap Junction ◽  
Systolic Function ◽  
Embryonic Stem ◽  
Immunohistochemical Analysis ◽  
Total Surface Area ◽  
Genetically Engineered ◽  
Chimeric Mice ◽  
Electrophysiological Properties ◽  
Junction Protein ◽  
Gap Junction Protein

Genetic studies in the mouse have demonstrated that conditional cardiac-restricted loss of connexin43 (Cx43), the major ventricular gap junction protein, is highly arrhythmogenic. However, whether more focal gap junction remodeling, as is commonly seen in acquired cardiomyopathies, influences the propensity for arrhythmogenesis is not known. We examined electrophysiological properties and the frequency of spontaneous and inducible arrhythmias in genetically engineered chimeric mice derived from injection of Cx43-deficient embryonic stem cells into wild-type recipient blastocysts. Chimeric mice had numerous well-circumscribed microscopic Cx43-negative foci in their hearts, comprising ∼15% of the total surface area as determined by immunohistochemical analysis. Systolic function in the chimeric mice was significantly depressed as measured echocardiographically (19.0% decline in fractional shortening compared with controls, P < 0.05) and by invasive hemodynamics (17.6% reduction in change of pressure over time, P < 0.01). Chimeras had significantly more spontaneous arrhythmic events than controls ( P < 0.01), including frequent runs of nonsustained ventricular tachycardia in some of the chimeric mice. However, in contrast to mice with conditional cardiac-resticted loss of Cx43 in the heart, no sustained ventricular tachyarrhythmias were observed. We conclude that focal areas of uncoupling in the myocardium increase the likelihood of arrhythmic triggers, but more widespread uncoupling is required to support sustained arrhythmias.

Differential regulation of gap junction protein expression in cardiomyocytes

2002 ◽  
Vol 2002 (Fall) ◽  
Author(s):  
Aida Salameh ◽  
Polin Mahjour ◽  
Katja M??hlberg ◽  
Andreas Hagendorff ◽  
Stefan Dhein ◽  
...  
Keyword(s):  
Protein Expression ◽  
Gap Junction ◽  
Differential Regulation ◽  
Junction Protein ◽  
Gap Junction Protein

scholarly journals Conduction abnormality in gap junction protein connexin45-deficient embryonic stem cell-derived cardiac myocytes

2004 ◽  
Vol 280A (2) ◽  
pp. 973-979 ◽  
Author(s):  
Katsuko Egashira ◽  
Kiyomasa Nishii ◽  
Kei-Ichiro Nakamura ◽  
Madoka Kumai ◽  
Sachio Morimoto ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Gap Junction ◽  
Cardiac Myocytes ◽  
Embryonic Stem ◽  
Conduction Abnormality ◽  
Junction Protein ◽  
Gap Junction Protein

Regulation of Gap Junction Protein (Connexin) Genes and Function in Differentiating ES Cells

2003 ◽  
pp. 63-69
Author(s):  
Masahito Oyamada ◽  
Yumiko Oyamada ◽  
Tomoyuki Kaneko ◽  
Tetsuro Takamatsu
Keyword(s):  
Gap Junction ◽  
Es Cells ◽  
Junction Protein ◽  
Gap Junction Protein ◽  
Connexin Genes ◽  
And Function

scholarly journals MP38 contains the membrane-embedded domain of the lens fiber gap junction protein MP70.

1990 ◽  
Vol 265 (22) ◽  
pp. 13357-13361
Author(s):  
J Kistler ◽  
J Schaller ◽  
H Sigrist
Keyword(s):  
Gap Junction ◽  
Lens Fiber ◽  
Junction Protein ◽  
Gap Junction Protein

Identification of a rat liver cDNA and mRNA coding for the 28 kDa gap junction protein

FEBS Letters ◽  
1986 ◽  
Vol 205 (1) ◽  
pp. 56-60 ◽  
Author(s):  
Roland Heynkes ◽  
Gisela Kozjek ◽  
Otto Traub ◽  
Klaus Willecke
Keyword(s):  
Gap Junction ◽  
Rat Liver ◽  
Junction Protein ◽  
Gap Junction Protein

scholarly journals Differential effect of subcellular localization of communication impairing gap junction protein connexin43 on tumor cell growth in vivo

Oncogene ◽  
2000 ◽  
Vol 19 (4) ◽  
pp. 505-513 ◽  
Author(s):  
V A Krutovskikh ◽  
S M Troyanovsky ◽  
C Piccoli ◽  
H Tsuda ◽  
M Asamoto ◽  
...  
Keyword(s):  
Cell Growth ◽  
Subcellular Localization ◽  
Tumor Cell ◽  
Gap Junction ◽  
Differential Effect ◽  
Tumor Cell Growth ◽  
Junction Protein ◽  
Gap Junction Protein
Sign in / Sign up

Export Citation Format

Share Document