scholarly journals Conduction abnormality in gap junction protein connexin45-deficient embryonic stem cell-derived cardiac myocytes

2004 ◽  
Vol 280A (2) ◽  
pp. 973-979 ◽  
Author(s):  
Katsuko Egashira ◽  
Kiyomasa Nishii ◽  
Kei-Ichiro Nakamura ◽  
Madoka Kumai ◽  
Sachio Morimoto ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Gap Junction ◽  
Cardiac Myocytes ◽  
Embryonic Stem ◽  
Conduction Abnormality ◽  
Junction Protein ◽  
Gap Junction Protein

scholarly journals MEKK1 suppresses oxidative stress-induced apoptosis of embryonic stem cell-derived cardiac myocytes

1999 ◽  
Vol 96 (26) ◽  
pp. 15127-15132 ◽  
Author(s):  
T. Minamino ◽  
T. Yujiri ◽  
P. J. Papst ◽  
E. D. Chan ◽  
G. L. Johnson ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cardiac Myocytes ◽  
Embryonic Stem ◽  
Induced Apoptosis

scholarly journals Focal gap junction uncoupling and spontaneous ventricular ectopy

2005 ◽  
Vol 289 (3) ◽  
pp. H1091-H1098 ◽  
Author(s):  
David E. Gutstein ◽  
Stephan B. Danik ◽  
Steve Lewitton ◽  
David France ◽  
Fangyu Liu ◽  
...  
Keyword(s):  
Gap Junction ◽  
Systolic Function ◽  
Embryonic Stem ◽  
Immunohistochemical Analysis ◽  
Total Surface Area ◽  
Genetically Engineered ◽  
Chimeric Mice ◽  
Electrophysiological Properties ◽  
Junction Protein ◽  
Gap Junction Protein

Genetic studies in the mouse have demonstrated that conditional cardiac-restricted loss of connexin43 (Cx43), the major ventricular gap junction protein, is highly arrhythmogenic. However, whether more focal gap junction remodeling, as is commonly seen in acquired cardiomyopathies, influences the propensity for arrhythmogenesis is not known. We examined electrophysiological properties and the frequency of spontaneous and inducible arrhythmias in genetically engineered chimeric mice derived from injection of Cx43-deficient embryonic stem cells into wild-type recipient blastocysts. Chimeric mice had numerous well-circumscribed microscopic Cx43-negative foci in their hearts, comprising ∼15% of the total surface area as determined by immunohistochemical analysis. Systolic function in the chimeric mice was significantly depressed as measured echocardiographically (19.0% decline in fractional shortening compared with controls, P < 0.05) and by invasive hemodynamics (17.6% reduction in change of pressure over time, P < 0.01). Chimeras had significantly more spontaneous arrhythmic events than controls ( P < 0.01), including frequent runs of nonsustained ventricular tachycardia in some of the chimeric mice. However, in contrast to mice with conditional cardiac-resticted loss of Cx43 in the heart, no sustained ventricular tachyarrhythmias were observed. We conclude that focal areas of uncoupling in the myocardium increase the likelihood of arrhythmic triggers, but more widespread uncoupling is required to support sustained arrhythmias.

Calcium Handling in Embryonic Stem Cell-Derived Cardiac Myocytes: Of Mice and Men

2006 ◽  
Vol 1080 (1) ◽  
pp. 207-215 ◽  
Author(s):  
I. ITZHAKI ◽  
J. SCHILLER ◽  
R. BEYAR ◽  
J. SATIN ◽  
L. GEPSTEIN
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cardiac Myocytes ◽  
Embryonic Stem ◽  
Calcium Handling ◽  
Of Mice And Men

Differential Regulation of Gap Junction Protein (Connexin) Genes during Cardiomyocytic Differentiation of Mouse Embryonic Stem Cellsin Vitro

1996 ◽  
Vol 229 (2) ◽  
pp. 318-326 ◽  
Author(s):  
Yumiko Oyamada ◽  
Kiyoshi Komatsu ◽  
Hisakazu Kimura ◽  
Michio Mori ◽  
Masahito Oyamada
Keyword(s):  
Gap Junction ◽  
Embryonic Stem ◽  
Differential Regulation ◽  
Junction Protein ◽  
Gap Junction Protein ◽  
Connexin Genes

02 Selection of embryonic stem cell-derived cardiac myocytes for transfer to myocardium

2002 ◽  
Vol 34 (7) ◽  
pp. A31
Author(s):  
Sarah Lodding ◽  
Michael Van Kanegan ◽  
Nancy Benkusky ◽  
Hector Valdivia ◽  
Gary Lyons
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cardiac Myocytes ◽  
Embryonic Stem ◽  
Selection Of

Gene transfer into mouse embryonic stem cell-derived cardiac myocytes mediated by recombinant adenovirus

1997 ◽  
Vol 33 (4) ◽  
pp. 270-276 ◽  
Author(s):  
Elizabeth M. Rust ◽  
Margaret V. Westfall ◽  
Linda C. Samuelson ◽  
Joseph M. Metzger
Keyword(s):  
Stem Cell ◽  
Gene Transfer ◽  
Embryonic Stem Cell ◽  
Cardiac Myocytes ◽  
Recombinant Adenovirus ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell
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