Phosphorylation sites of mouse gap junction protein 31 were determined by mass spectrometry

2019 ◽  
Vol 1 (1) ◽  
pp. 18-27
Author(s):  
Ma Junjie
Keyword(s):  
Mass Spectrometry ◽  
Gap Junction ◽  
Phosphorylation Sites ◽  
Junction Protein ◽  
Gap Junction Protein

scholarly journals Ezrin-anchored PKA phosphorylates serine 369 and 373 on connexin 43 to enhance gap junction assembly, communication, and cell fusion

2018 ◽  
Vol 475 (2) ◽  
pp. 455-476 ◽  
Author(s):  
Aleksandra R. Dukic ◽  
Pascale Gerbaud ◽  
Jean Guibourdenche ◽  
Bernd Thiede ◽  
Kjetil Taskén ◽  
...  
Keyword(s):  
Gap Junction ◽  
Cell Fusion ◽  
Connexin 43 ◽  
Phosphorylation Sites ◽  
Peptide Arrays ◽  
Signaling Complex ◽  
Gap Junction Communication ◽  
Junction Protein ◽  
Gap Junction Protein

A limited number of human cells can fuse to form multinucleated syncytia. In the differentiation of human placenta, mononuclear cytotrophoblasts fuse to form an endocrinologically active, non-proliferative, multinucleated syncytium. This syncytium covers the placenta and manages the exchange of nutrients and gases between maternal and fetal circulation. We recently reported protein kinase A (PKA) to be part of a macromolecular signaling complex with ezrin and gap junction protein connexin 43 (Cx43) that provides cAMP-mediated control of gap junction communication. Here, we examined the associated phosphorylation events. Inhibition of PKA activity resulted in decreased Cx43 phosphorylation, which was associated with reduced trophoblast fusion and differentiation. In vitro studies using peptide arrays, together with mass spectrometry, pointed to serine 369 and 373 of Cx43 as the major PKA phosphorylation sites that increases gap junction assembly at the plasmalemma. A combination of knockdown and reconstitution experiments and gap-fluorescence loss in photobleaching assays with mutant Cx43 containing single or double phosphoserine-mimicking amino acid substitutions in putative PKA phosphorylation sites demonstrated that phosphorylation of S369 and S373 mediated gap junction communication, trophoblast differentiation, and cell fusion.

scholarly journals Characterization of the Mitogen-activated Protein Kinase Phosphorylation Sites on the Connexin-43 Gap Junction Protein

1996 ◽  
Vol 271 (7) ◽  
pp. 3779-3786 ◽  
Author(s):  
Bonnie J. Warn-Cramer ◽  
Paul D. Lampe ◽  
Wendy E. Kurata ◽  
Martha Y. Kanemitsu ◽  
Lenora W. M. Loo ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Gap Junction ◽  
Connexin 43 ◽  
Mitogen Activated Protein Kinase ◽  
Phosphorylation Sites ◽  
Junction Protein ◽  
Mitogen Activated Protein ◽  
Gap Junction Protein ◽  
Kinase Phosphorylation

scholarly journals MP38 contains the membrane-embedded domain of the lens fiber gap junction protein MP70.

1990 ◽  
Vol 265 (22) ◽  
pp. 13357-13361
Author(s):  
J Kistler ◽  
J Schaller ◽  
H Sigrist
Keyword(s):  
Gap Junction ◽  
Lens Fiber ◽  
Junction Protein ◽  
Gap Junction Protein

Identification of a rat liver cDNA and mRNA coding for the 28 kDa gap junction protein

FEBS Letters ◽  
1986 ◽  
Vol 205 (1) ◽  
pp. 56-60 ◽  
Author(s):  
Roland Heynkes ◽  
Gisela Kozjek ◽  
Otto Traub ◽  
Klaus Willecke
Keyword(s):  
Gap Junction ◽  
Rat Liver ◽  
Junction Protein ◽  
Gap Junction Protein

scholarly journals Differential effect of subcellular localization of communication impairing gap junction protein connexin43 on tumor cell growth in vivo

Oncogene ◽  
2000 ◽  
Vol 19 (4) ◽  
pp. 505-513 ◽  
Author(s):  
V A Krutovskikh ◽  
S M Troyanovsky ◽  
C Piccoli ◽  
H Tsuda ◽  
M Asamoto ◽  
...  
Keyword(s):  
Cell Growth ◽  
Subcellular Localization ◽  
Tumor Cell ◽  
Gap Junction ◽  
Differential Effect ◽  
Tumor Cell Growth ◽  
Junction Protein ◽  
Gap Junction Protein

scholarly journals Role of Gap Junction Protein Connexin43 in Astrogliosis Induced by Brain Injury

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47311 ◽  
Author(s):  
Nicolas Theodoric ◽  
John F. Bechberger ◽  
Christian C. Naus ◽  
Wun-Chey Sin
Keyword(s):  
Brain Injury ◽  
Gap Junction ◽  
Junction Protein ◽  
Gap Junction Protein

scholarly journals Connexin 43 Gap Junction Protein Expression during Follicular Development in the Porcine Ovary1

1998 ◽  
Vol 58 (2) ◽  
pp. 583-590 ◽  
Author(s):  
Judy A. Lenhart ◽  
Bruce R. Downey ◽  
Carol A. Bagnell
Keyword(s):  
Protein Expression ◽  
Gap Junction ◽  
Connexin 43 ◽  
Follicular Development ◽  
Junction Protein ◽  
Gap Junction Protein

Specific localisation of gap junction protein connexin 32 in the gastric mucosa of horses

2005 ◽  
Vol 125 (3) ◽  
pp. 307-313 ◽  
Author(s):  
Cornelia Fink ◽  
Tanja Hembes ◽  
Ralph Brehm ◽  
Roswitha Weigel ◽  
Cornelia Heeb ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Gap Junction ◽  
Connexin 32 ◽  
Junction Protein ◽  
Gap Junction Protein
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