Reduced Susceptibility of Magnocellular Neuroendocrine Nuclei of the Rat Hypothalamus to Transient Focal Ischemia Produced by Middle Cerebral Artery Occlusion

2002 ◽  
Vol 178 (2) ◽  
pp. 268-279 ◽  
Author(s):  
M CURRASCOLLAZO
Keyword(s):  
Middle Cerebral Artery ◽  
Middle Cerebral Artery Occlusion ◽  
Cerebral Artery ◽  
Focal Ischemia ◽  
Artery Occlusion ◽  
Rat Hypothalamus ◽  
Transient Focal Ischemia ◽  
Cerebral Artery Occlusion

scholarly journals Transient Focal Ischemia Induces Extensive Temporal Changes in Rat Cerebral MicroRNAome

2009 ◽  
Vol 29 (4) ◽  
pp. 675-687 ◽  
Author(s):  
Ashuthosh Dharap ◽  
Kellie Bowen ◽  
Robert Place ◽  
Long-Cheng Li ◽  
Raghu Vemuganti
Keyword(s):  
Middle Cerebral Artery ◽  
Middle Cerebral Artery Occlusion ◽  
Cerebral Artery ◽  
Noncoding Rna ◽  
Critical Role ◽  
In Silico Analysis ◽  
Focal Ischemia ◽  
Artery Occlusion ◽  
Mirna Biogenesis ◽  
Cerebral Artery Occlusion

MicroRNAs (miRNAs) are ∼22 nucleotides long, noncoding RNAs that control cellular function by either degrading mRNAs or arresting their translation. To understand their functional significance in ischemic pathophysiology, we profiled miRNAs in adult rat brain as a function of reperfusion time after transient middle cerebral artery occlusion. Of the 238 miRNAs evaluated, 8 showed increased and 12 showed decreased expression at least at 4 out of 5 reperfusion time points studied between 3 h and 3 days compared with sham. Of those, 17 showed > 5 fold change. Bioinformatics analysis indicated a correlation between miRNAs altered to several mRNAs known to mediate inflammation, transcription, neuroprotection, receptors function, and ionic homeostasis. Antagomir-mediated prevention of mir-145 expression led to an increased protein expression of its downstream target superoxide dismutase-2 in the postischemic brain. In silico analysis showed sequence complementarity of eight miRNAs induced after focal ischemia to 877 promoters indicating the possibility of noncoding RNA-induced activation of gene expression. The mRNA expression of the RNases Drosha and Dicer, cofactor Pasha, and the pre-miRNA transporter exportin-5, which modulate miRNA biogenesis, were not altered after transient middle cerebral artery occlusion. Thus, the present studies indicate a critical role of miRNAs in controlling mRNA transcription and translation in the postischemic brain.

Sign in / Sign up

Export Citation Format

Share Document