Development and Characterization of a Genetic Linkage Map of Cryptococcus neoformans var. neoformans Using Amplified Fragment Length Polymorphisms and Other Markers

Abstract We have constructed a genetic linkage map for the parasitic protozoan, Toxoplasma gondii, using randomly selected low copy number DNA markers that define restriction fragment length polymorphisms (RFLPs). The inheritance patterns of 64 RFLP markers and two phenotypic markers were analyzed among 19 recombinant haploid progeny selected from two parallel genetic crosses between PLK and CEP strains. In these first successful interstrain crosses, these RFLP markers segregated into 11 distinct genetic linkage groups that showed close correlation with physical linkage groups previously defined by molecular karyotype. Separate linkage maps, constructed for each of the 11 chromosomes, indicated recombination frequencies range from approximately 100 to 300 kb per centimorgan. Preliminary linkage assignments were made for the loci regulating sinefungin resistance (snf-1) on chromosome IX and adenine arabinoside (ara-1) on chromosome V by linkage to RFLP markers. Despite random segregation of separate chromosomes, the majority of chromosomes failed to demonstrate internal recombination events and in 3/19 recombinant progeny no intramolecular recombination events were detected. The relatively low rate of intrachromosomal recombination predicts that tight linkage for unknown genes can be established with a relatively small set of markers. This genetic linkage map should prove useful in mapping genes that regulate drug resistance and other biological phenotypes in this important opportunistic pathogen.

Download Full-text

A reference cross DNA panel for zebrafish (Danio rerio) anchored with simple sequence length polymorphisms

Development ◽

10.1242/dev.123.1.451 ◽

1996 ◽

Vol 123 (1) ◽

pp. 451-460 ◽

Cited By ~ 8

Author(s):

E.W. Knapik ◽

A. Goodman ◽

O.S. Atkinson ◽

C.T. Roberts ◽

M. Shiozawa ◽

...

Keyword(s):

Linkage Map ◽

Genetic Markers ◽

Genetic Linkage ◽

Positional Cloning ◽

Genetic Linkage Map ◽

Physical Map ◽

Sequence Length ◽

Mapping Tool ◽

Length Polymorphisms ◽

Simple Sequence

The ultimate informativeness of the zebrafish mutations described in this issue will rest in part on the ability to clone these genes. However, the genetic infrastructure required for the positional cloning in zebrafish is still in its infancy. Here we report a reference cross panel of DNA, consisting of 520 F2 progeny (1040 meioses) that has been anchored to a zebrafish genetic linkage map by 102 simple sequence length polymorphisms. This reference cross DNA provides: (1) a panel of DNA from the cross that was used to construct the genetic linkage map, upon which polymorphic gene(s) and genetic markers can be mapped; (2) a fine order mapping tool, with a maximum resolution of 0.1 cM; and (3) a foundation for the development of a physical map (an ordered array of clones each containing a known portion of the genome). This reference cross DNA will serve as a resource enabling investigators to relate genes or genetic markers directly to a single genetic linkage map and avoid the problem of integrating different maps with different genetic markers, as must be currently done when using randomly amplified polymorphic DNA markers, or as has occurred with human genetic linkage maps.

Download Full-text

Characterization of Morphological Traits Based on a Genetic Linkage Map in Peach.

Breeding Science ◽

10.1270/jsbbs.51.271 ◽

2001 ◽

Vol 51 (4) ◽

pp. 271-278 ◽

Cited By ~ 71

Author(s):

Toshiya Yamamoto ◽

Takehiko Shimada ◽

Tsuyoshi Imai ◽

Hideaki Yaegaki ◽

Takashi Haji ◽

...

Keyword(s):

Linkage Map ◽

Genetic Linkage ◽

Morphological Traits ◽

Genetic Linkage Map

Download Full-text

MOLECULAR CHARACTERIZATION OF IRANIAN ALMOND CULTIVARS AND RELATED WILD SPECIES USING AMPLIFIED FRAGMENT-LENGTH POLYMORPHISMS (AFLPS)

Acta Horticulturae ◽

10.17660/actahortic.2009.814.16 ◽

2009 ◽

pp. 137-142 ◽

Cited By ~ 2

Author(s):

B. Shiran ◽

K. Sorkheh ◽

V. Rouhi ◽

T.M. Gradziel ◽

P. Martínez-Gómez

Keyword(s):

Molecular Characterization ◽

Fragment Length ◽

Wild Species ◽

Length Polymorphisms ◽

Amplified Fragment Length Polymorphisms ◽

Related Wild Species

Download Full-text

Linkage map of birch, Betula pendula Roth, based on microsatellites and amplified fragment length polymorphisms

Genome ◽

10.1139/g05-031 ◽

2005 ◽

Vol 48 (4) ◽

pp. 619-625 ◽

Cited By ~ 22

Author(s):

M Pekkinen ◽

S Varvio ◽

K K.M Kulju ◽

H Kärkkäinen ◽

S Smolander ◽

...

Keyword(s):

Linkage Map ◽

Fragment Length ◽

Betula Pendula ◽

Linkage Data ◽

Linkage Groups ◽

Betula Pendula Roth ◽

Mapping Strategy ◽

Length Polymorphisms ◽

Amplified Fragment Length Polymorphisms ◽

Map Distance

The first genetic linkage map for silver birch, Betula pendula Roth, was constructed by using a pseudo-testcross mapping strategy and integration of linkage data from 3 unrelated 2-generation pedigrees. The map is based on the genetic inheritance and segregation of 82 amplified fragment length polyhmorphisms and 19 microsatellite markers, and was constructed by simultaneously comparing the performance of CRI-MAP and OUTMAP packages. The analysis revealed 16 linkage groups, and the total map coverage is 1561 cM (Kosambi units). Average map distance between adjacent markers is 15.5 cM. Linkage groups range between 6 and 18 loci and from 81.2 to 326.5 cM; the remaining 9 linkage groups consist of 2 or 3 loci ranging from 6.3 to 42.4 cM. The uncertainty of the map is illustrated with sensitivity analysis. This initial map can serve as a basis for developing a more detailed genetic map.Key words: Betula pendula, linkage map, microsatellite, AFLP, CRI-MAP, OUTMAP.

Download Full-text