A reference cross DNA panel for zebrafish (Danio rerio) anchored with simple sequence length polymorphisms

Development ◽  
1996 ◽  
Vol 123 (1) ◽  
pp. 451-460 ◽  
Author(s):  
E.W. Knapik ◽  
A. Goodman ◽  
O.S. Atkinson ◽  
C.T. Roberts ◽  
M. Shiozawa ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Markers ◽  
Genetic Linkage ◽  
Positional Cloning ◽  
Genetic Linkage Map ◽  
Physical Map ◽  
Sequence Length ◽  
Mapping Tool ◽  
Length Polymorphisms ◽  
Simple Sequence

The ultimate informativeness of the zebrafish mutations described in this issue will rest in part on the ability to clone these genes. However, the genetic infrastructure required for the positional cloning in zebrafish is still in its infancy. Here we report a reference cross panel of DNA, consisting of 520 F2 progeny (1040 meioses) that has been anchored to a zebrafish genetic linkage map by 102 simple sequence length polymorphisms. This reference cross DNA provides: (1) a panel of DNA from the cross that was used to construct the genetic linkage map, upon which polymorphic gene(s) and genetic markers can be mapped; (2) a fine order mapping tool, with a maximum resolution of 0.1 cM; and (3) a foundation for the development of a physical map (an ordered array of clones each containing a known portion of the genome). This reference cross DNA will serve as a resource enabling investigators to relate genes or genetic markers directly to a single genetic linkage map and avoid the problem of integrating different maps with different genetic markers, as must be currently done when using randomly amplified polymorphic DNA markers, or as has occurred with human genetic linkage maps.

scholarly journals Integration of the Aedes aegypti Mosquito Genetic Linkage and Physical Maps

Genetics ◽  
2001 ◽  
Vol 157 (3) ◽  
pp. 1299-1305
Author(s):  
S E Brown ◽  
D W Severson ◽  
L A Smith ◽  
D L Knudson
Keyword(s):  
Aedes Aegypti ◽  
Linkage Map ◽  
Genetic Linkage ◽  
Positional Cloning ◽  
Genetic Linkage Map ◽  
Physical Map ◽  
Chromosome 1 ◽  
Chromosome 2 ◽  
Metaphase Chromosomes ◽  
Physical Maps

Abstract Two approaches were used to correlate the Aedes aegypti genetic linkage map to the physical map. STS markers were developed for previously mapped RFLP-based genetic markers so that large genomic clones from cosmid libraries could be found and placed to the metaphase chromosome physical maps using standard FISH methods. Eight cosmids were identified that contained eight RFLP marker sequences, and these cosmids were located on the metaphase chromosomes. Twenty-one cDNAs were mapped directly to metaphase chromosomes using a FISH amplification procedure. The chromosome numbering schemes of the genetic linkage and physical maps corresponded directly and the orientations of the genetic linkage maps for chromosomes 2 and 3 were inverted relative to the physical maps. While the chromosome 2 linkage map represented essentially 100% of chromosome 2, ∼65% of the chromosome 1 linkage map mapped to only 36% of the short p-arm and 83% of the chromosome 3 physical map contained the complete genetic linkage map. Since the genetic linkage map is a RFLP cDNA-based map, these data also provide a minimal estimate for the size of the euchromatic regions. The implications of these findings on positional cloning in A. aegypti are discussed.

A genetic map of the mouse suitable for typing intraspecific crosses.

Genetics ◽  
1992 ◽  
Vol 131 (2) ◽  
pp. 423-447 ◽  
Author(s):  
W Dietrich ◽  
H Katz ◽  
S E Lincoln ◽  
H S Shin ◽  
J Friedman ◽  
...  
Keyword(s):  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Mouse Genome ◽  
Sequence Length ◽  
Chain Reaction ◽  
Average Spacing ◽  
Length Polymorphisms ◽  
Polymerase Chain ◽  
High Degree ◽  
Simple Sequence

Abstract We report the construction of a genetic linkage map of the mouse, consisting entirely of genetic markers that can be rapidly typed by polymerase chain reaction and that show a high degree of polymorphism among inbred laboratory strains. Specifically, the map contains 317 simple sequence length polymorphisms at an average spacing of 4.3 cM and is detectably linked to approximately 99% of the mouse genome. In typical crosses between inbred laboratory strains, about 50% of the markers are polymorphic, making it straightforward to follow inheritance in almost any cross.

scholarly journals A Dense Genetic Linkage Map for Common Carp and Its Integration with a BAC-Based Physical Map

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e63928 ◽  
Author(s):  
Lan Zhao ◽  
Yan Zhang ◽  
Peifeng Ji ◽  
Xiaofeng Zhang ◽  
Zixia Zhao ◽  
...  
Keyword(s):  
Linkage Map ◽  
Common Carp ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Physical Map

A cytogenetically based physical map of chromosome 1B in common wheat

Genome ◽  
1993 ◽  
Vol 36 (3) ◽  
pp. 548-554 ◽  
Author(s):  
R. S. Kota ◽  
K. S. Gill ◽  
B. S. Gill ◽  
T. R. Endo
Keyword(s):  
Genetic Markers ◽  
Common Wheat ◽  
Hexaploid Wheat ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Physical Map ◽  
Wheat Chromosome ◽  
Homozygous Deletion ◽  
Genetic Maps ◽  
Rflp Markers

We have constructed a cytogenetically based physical map of chromosome 1B in common wheat by utilizing a total of 18 homozygous deletion stocks. It was possible to divide chromosome 1B into 17 subregions. Nineteen genetic markers are physically mapped to nine subregions of chromosome 1B. Comparison of the cytological map of chromosome 1B with an RFLP-based genetic linkage map of Triticum tauschii revealed that the linear order of the genetic markers was maintained between chromosome 1B of hexaploid wheat and 1D of T. tauschii. Striking differences were observed between the physical and genetic maps in relation to the relative distances between the genetic markers. The genetic markers clustered in the middle of the genetic map were physically located in the distal regions of both arms of chromosome 1B. It is unclear whether the increased recombination in the distal regions of chromosome 1B is due to specific regions of increased recombination or a more broadly distributed increase in recombination in the distal regions of Triticeae chromosomes.Key words: common wheat, chromosome 1B, homozygous deletion lines, physical map, RFLP markers.

Microsatellite and amplified sequence length polymorphisms in cultivated and wild soybean

Genome ◽  
1995 ◽  
Vol 38 (4) ◽  
pp. 715-723 ◽  
Author(s):  
P. J. Maughan ◽  
M. A. Saghai Maroof ◽  
G. R. Buss
Keyword(s):  
Genetic Markers ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Allelic Diversity ◽  
Sequence Length ◽  
Length Polymorphisms ◽  
Ssr Loci ◽  
Low Levels ◽  
Cultivated Soybean ◽  
Simple Sequence

The objectives of this study were to (i) assess the extent of genetic variation in soybean microsatellites (simple sequence repeats or SSRs), (ii) assay for amplified sequence length polymorphisms (ASLPs), and (iii) evaluate the usefulness of SSRs and ASLPs as genetic markers. Five microsatellites detected a total of 79 variants (alleles) in a sample of 94 accessions of wild (Glycine soja) and cultivated soybean (G. max). F2 segregation analysis of four of the five microsatellites identified these variants (alleles) with four loci located in independent linkage groups. The number of alleles per microsatellite locus ranged from 5 to 21; to our knowledge these are the largest numbers of alleles for single Mendelian loci reported in soybean. Allelic diversity for the SSR loci was greater in wild than in cultivated soybean. Overall, 43 more SSR alleles were detected in wild than in cultivated soybean. These results indicate that SSRs are the marker of choice, especially for species with low levels of variation as detected by other types of markers. Two alleles were detected at each of the three ASLP loci examined. A total of six ASLP alleles were observed in cultivated soybean and five were observed in wild soybean; all alleles detected in wild soybean were present in cultivated soybean. Allelic diversity values for the ASLP loci were near previous estimates for restriction fragment length polymorphisms and therefore ASLPs may be useful as genetic markers in site-directed mapping.Key words: microsatellite, simple sequence repeat, soybean, amplified sequence length polymorphism, genetic mapping.

First simple sequence repeat-based genetic linkage map reveals a major QTL for leafing time in walnut (Juglans regia L.)

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Sina Kefayati ◽  
Adi Surya Ikhsan ◽  
Mehmet Sutyemez ◽  
Aibibula Paizila ◽  
Hayat Topçu ◽  
...  
Keyword(s):  
Linkage Map ◽  
Simple Sequence Repeat ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Juglans Regia ◽  
Sequence Repeat ◽  
Simple Sequence ◽  
Major Qtl
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