Cloning and Expression of Somatolactin, a Pituitary Hormone Related to Growth Hormone and Prolactin from Gilthead Seabream,Sparus aurata

ABSTRACT A heterologous radioimmunoassay (RIA) was applied for the determination of immunoreactive (IR)insulin-like growth factor (IGF-I) in a teleost, the gilthead seabream (Sparus aurata). Serial dilutions of the fish plasma gave a linear curve when added to constant amounts of 125I-labelled human IGF-I(53-70) and antiserum prepared against this fragment. The RIA was used to study the effect of GH on plasma levels of IR-IGF-I in S. aurata. A single injection of human recombinant GH (1 μg/g) resulted in a significant increase in IR-IGF-I at 29, 48 and 72 h, when compared with saline-injected fish. This novel observation suggests that in fish, as in mammals, circulating IGF-I levels are modulated by GH.

Download Full-text

Effect of growth hormone on the growth rate of the gilthead seabream (Sparus aurata), and use of different constructs for the production of transgenic fish

Aquaculture ◽

10.1016/0044-8486(93)90036-x ◽

1993 ◽

Vol 111 (1-4) ◽

pp. 189-197 ◽

Cited By ~ 25

Author(s):

Benzion Cavari ◽

Bruria Funkenstein ◽

Thomas T. Chen ◽

Lucia Irene Gonzalez-Villasenor ◽

Manfred Schartl

Keyword(s):

Growth Hormone ◽

Growth Rate ◽

Sparus Aurata ◽

Transgenic Fish ◽

Gilthead Seabream

Download Full-text

Preparation of Recombinant Gilthead Seabream (Sparus aurata) Growth Hormone and Its Use for Stimulation of Larvae Growth by Oral Administration

General and Comparative Endocrinology ◽

10.1006/gcen.1998.7192 ◽

1999 ◽

Vol 113 (1) ◽

pp. 155-164 ◽

Cited By ~ 29

Author(s):

I Ben-Atia ◽

M Fine ◽

A Tandler ◽

B Funkenstein ◽

S Maurice ◽

...

Keyword(s):

Growth Hormone ◽

Oral Administration ◽

Sparus Aurata ◽

Gilthead Seabream ◽

Stimulation Of

Download Full-text

Genomic structure and sequence of the gilthead seabream (Sparus aurata) growth hormone-encoding gene: Identification of minisatellite polymorphism in intron I

Genome ◽

10.1139/g00-051 ◽

2000 ◽

Vol 43 (5) ◽

pp. 836-845 ◽

Cited By ~ 39

Author(s):

R Almuly ◽

B Cavari ◽

H Ferstman ◽

O Kolodny ◽

B Funkenstein

Keyword(s):

Growth Hormone ◽

Tandem Repeats ◽

Sparus Aurata ◽

Genomic Structure ◽

Pcr Analysis ◽

Gilthead Seabream ◽

Growth Hormone Gene ◽

Response Element ◽

First Intron ◽

Gh Gene

The growth hormone (GH) gene of the gilthead seabream (Sparus aurata) (saGH) has been cloned, sequenced, and characterized. The saGH gene spans approximately 4.3 kb and consists of six exons and five introns, as found for all cloned teleost GH genes with the exception of carps and catfish. The first and third introns contain long stretches of repetitive tandem repeats. The second intron, which is unusually long compared with that in other teleosts (and other vertebrates) spans 1747 nucleotides (nt) and contains several inverted repeats. Intron-targeted polymerase chain reaction (PCR) analysis identified length polymorphism of the first intron. Sequence analysis of four variants (405, 424, 636, and 720 nt) out of many variants found revealed that the variation in length is due to differences in the number of repeat monomers (17-mer or 15-mer) as well as minor changes in their length. This repeat unit contains the consensus half-site motif of the thyroid hormone response element (TRE) and estrogen response element (ERE). Polymorphism was found also in the third intron. This is the first report of such high polymorphism of the first intron of GH gene in a vertebrate.Key words: growth hormone, gene, intron polymorphism, fish, Sparus aurata.

Download Full-text