The NMR Solution Structure of Intestinal Fatty Acid-binding Protein Complexed with Palmitate: Application of a Novel Distance Geometry Algorithm

1996 ◽  
Vol 264 (3) ◽  
pp. 585-602 ◽  
Author(s):  
Michael E. Hodsdon ◽  
Jay W. Ponder ◽  
David P. Cistola
Keyword(s):  
Fatty Acid ◽  
Solution Structure ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Distance Geometry ◽  
Nmr Solution Structure ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

Solution structure of bovine heart fatty acid-binding protein (H-FABPC)

1993 ◽  
pp. 15-22
Author(s):  
Dirck Lassen ◽  
Christian Lücke ◽  
Arno Kromminga ◽  
Axel Lezius ◽  
Friedrich Spener ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Solution Structure ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Bovine Heart

Solution structure of bovine heart fatty acid-binding protein (H-FABPc)

1993 ◽  
Vol 123 (1-2) ◽  
pp. 15-22 ◽  
Author(s):  
Dirck Lassen ◽  
Christian L�cke ◽  
Arno Kromminga ◽  
Axel Lezius ◽  
Friedrich Spener ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Solution Structure ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Bovine Heart

scholarly journals Solution Structure and Backbone Dynamics of Human Liver Fatty Acid Binding Protein: Fatty Acid Binding Revisited

2012 ◽  
Vol 102 (11) ◽  
pp. 2585-2594 ◽  
Author(s):  
Jun Cai ◽  
Christian Lücke ◽  
Zhongjing Chen ◽  
Ye Qiao ◽  
Elena Klimtchuk ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Human Liver ◽  
Solution Structure ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Backbone Dynamics ◽  
Liver Fatty Acid ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

scholarly journals Solution structure and backbone dynamics of human epidermal-type fatty acid-binding protein (E-FABP)

2002 ◽  
Vol 364 (3) ◽  
pp. 725-737 ◽  
Author(s):  
Luis H. GUTIÉRREZ-GONZÁLEZ ◽  
Christian LUDWIG ◽  
Carsten HOHOFF ◽  
Martin RADEMACHER ◽  
Thorsten HANHOFF ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Solution Structure ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Lipid Binding ◽  
Backbone Dynamics ◽  
Time Range ◽  
Fatty Acid Binding ◽  
Lipid Binding Protein ◽  
Acid Binding Protein

Human epidermal-type fatty acid-binding protein (E-FABP) belongs to a family of intracellular 14–15kDa lipid-binding proteins, whose functions have been associated with fatty acid signalling, cell growth, regulation and differentiation. As a contribution to understanding the structure—function relationship, we report in the present study features of its solution structure and backbone dynamics determined by NMR spectroscopy. Applying multi-dimensional high-resolution NMR techniques on unlabelled and 15N-enriched recombinant human E-FABP, the 1H and 15N resonance assignments were completed. On the basis of 2008 distance restraints, the three-dimensional solution structure of human E-FABP was subsequently obtained (backbone atom root-mean-square deviation of 0.92±0.11Å; where 1Å = 0.1nm), consisting mainly of 10 anti-parallel β-strands that form a β-barrel structure. 15N relaxation experiments (T1, T2 and heteronuclear nuclear Overhauser effects) at 500, 600 and 800MHz provided information on the internal dynamics of the protein backbone. Nearly all non-terminal backbone amide groups showed order parameters S2>0.8, with an average value of 0.88±0.04, suggesting a uniformly low backbone mobility in the nanosecond-to-picosecond time range. Moreover, hydrogen/deuterium exchange experiments indicated a direct correlation between the stability of the hydrogen-bonding network in the β-sheet structure and the conformational exchange in the millisecond-to-microsecond time range. The features of E-FABP backbone dynamics elaborated in the present study differ markedly from those of the phylogenetically closely related heart-type FABP and the more distantly related ileal lipid-binding protein, implying a strong interdependence with the overall protein stability and possibly also with the ligand-binding affinity for members of the lipid-binding protein family.

Solution structure of fatty acid-binding protein from human brain

2002 ◽  
pp. 61-68
Author(s):  
Martin Rademacher ◽  
Aukje W. Zimmerman ◽  
Heinz Rüterjans ◽  
Jacques H. Veerkamp ◽  
Christian Lücke
Keyword(s):  
Fatty Acid ◽  
Human Brain ◽  
Solution Structure ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

scholarly journals Solution Structure and Backbone Dynamics of Human Liver Fatty Acid Binding Protein

2010 ◽  
Vol 98 (3) ◽  
pp. 238a ◽  
Author(s):  
Jun Cai ◽  
Christian Lücke ◽  
Ye Qiao ◽  
Elena Klimtchuk ◽  
James A. Hamilton
Keyword(s):  
Fatty Acid ◽  
Human Liver ◽  
Solution Structure ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Backbone Dynamics ◽  
Liver Fatty Acid ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

scholarly journals Human Liver Fatty Acid Binding Protein: Solution Structure and Ligand Binding

2009 ◽  
Vol 96 (3) ◽  
pp. 600a ◽  
Author(s):  
Jun Cai ◽  
Christian Lücker ◽  
Zhongjing Chen ◽  
Elena Klimtchuk ◽  
Ye Qiao ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Ligand Binding ◽  
Human Liver ◽  
Solution Structure ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Liver Fatty Acid ◽  
Protein Solution ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

Verminderte Adipocyte fatty acid binding protein (AFABP)-Spiegel bei Patienten mit chronischer Pankreatitis

2011 ◽  
Vol 49 (08) ◽  
Author(s):  
L Selig ◽  
T Ebert ◽  
M Reiche ◽  
D Krehl ◽  
U Lossner ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

Calcium-dependent release of adipocyte fatty acid binding protein from human adipocytes

2014 ◽  
Vol 122 (03) ◽  
Author(s):  
I Schlottmann ◽  
M Ehrhart-Bornstein ◽  
M Wabitsch ◽  
SR Bornstein ◽  
V Lamounier-Zepter
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Human Adipocytes ◽  
Fatty Acid Binding ◽  
Calcium Dependent ◽  
Acid Binding Protein
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