Genetic analysis of an archaeal Holliday junction resolvase in Escherichia coli 1 1Edited by J. Karn

2001 ◽  
Vol 310 (3) ◽  
pp. 577-589 ◽  
Author(s):  
Edward L Bolt ◽  
Robert G Lloyd ◽  
Gary J Sharples
Keyword(s):  
Escherichia Coli ◽  
Genetic Analysis ◽  
Holliday Junction ◽  
Holliday Junction Resolvase

scholarly journals The search for a human Holliday junction resolvase

2009 ◽  
Vol 37 (3) ◽  
pp. 519-526 ◽  
Author(s):  
Stephen C. West
Keyword(s):  
Escherichia Coli ◽  
Tissue Culture ◽  
Chromosome Segregation ◽  
Meiotic Recombination ◽  
Human Cells ◽  
Holliday Junctions ◽  
Holliday Junction ◽  
Brute Force ◽  
Holliday Junction Resolvase

Four-way DNA intermediates, known as Holliday junctions, are formed during mitotic and meiotic recombination, and their efficient resolution is essential for proper chromosome segregation. Bacteria, bacteriophages and archaea promote Holliday junction resolution by the introduction of symmetrically related nicks across the junction, in reactions mediated by Holliday junction resolvases. In 2008, after a search that lasted almost 20 years, a Holliday junction resolvase was identified in humans. The protein, GEN1, was identified using MS following the brute-force fractionation of extracts prepared from human cells grown in tissue culture. GEN1 fits the paradigm developed from studies of prokaryotic Holliday junction resolvases, in that it specifically recognizes junctions and resolves them using a mechanism similar to that exhibited by the Escherichia coli RuvC protein.

Further genetic analysis of the C-terminal external loop region in Escherichia coli maltoporin

1997 ◽  
Vol 148 (5) ◽  
pp. 375-387 ◽  
Author(s):  
P.E. Klebba ◽  
S.M.C. Newton ◽  
A. Charbit ◽  
V. Michel ◽  
D. Perrin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetic Analysis ◽  
External Loop ◽  
Loop Region

A Test of the Double-Strand Break Repair Model for Meiotic Recombination in Saccharomyces cerevisiae

Genetics ◽  
1996 ◽  
Vol 144 (1) ◽  
pp. 27-41 ◽  
Author(s):  
Larry A Gilbertson ◽  
Franklin W Stahl
Keyword(s):  
Meiotic Recombination ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Holliday Junction ◽  
Single Event ◽  
Heteroduplex Dna ◽  
Holliday Junction Resolvase ◽  
Break Repair ◽  
Two Sides

Abstract We tested predictions of the double-strand break repair (DSBR) model for meiotic recombination by examining the segregation patterns of small palindromic insertions, which frequently escape mismatch repair when in heteroduplex DNA. The palindromes flanked a well characterized DSB site at the ARC4 locus. The “canonical” DSBR model, in which only 5′ ends are degraded and resolution of the four-stranded intermediate is by Holliday junction resolvase, predicts that hDNA will frequently occur on both participating chromatids in a single event. Tetrads reflecting this configuration of hDNA were rare. In addition, a class of tetrads not predicted by the canonical DSBR model was identified. This class represented events that produced hDNA in a “trans” configuration, on opposite strands of the same duplex on the two sides of the DSB site. Whereas most classes of convertant tetrads had typical frequencies of associated crossovers, tetrads with trans hDNA were parental for flanking markers. Modified versions of the DSBR model, including one that uses a topoisomerase to resolve the canonical DSBR intermediate, are supported by these data.

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