Mitochondrial 12S rDNA Sequence Relationships Suggest That the Enigmatic Bovid “Linh Duong” Pseudonovibos spiralis Is Closely Related to Buffalo

Abstract Objectives Annually, 1.2 million humans are stung by scorpions and severely affected by their venom. Some of the scorpion species of medical importance have a similar morphology to species with low toxicity. To establish diagnostic tools for surveying scorpions, the current study was conducted to generate three mitochondrial markers, Cytochrome Oxidase I (COI gene), 12S rDNA and 16S rDNA for six species of medically important Iranian scorpions: Androctonus crassicauda, Hottentotta saulcyi, Mesobuthus caucasicus, M. eupeus, Odontobuthus doriae, and Scorpio maurus. Results Phylogenetic analyses of the obtained sequences corroborated the morphological identification. For the first time, 12S rDNA sequences are reported from Androctonus crassicauda, Hottentotta saulcyi, Mesobuthus caucasicus and M. eupeus and also the 16S rDNA sequence from Hottentotta saulcyi. We conclude that the mitochondrial markers are useful for species determination among these medically important species of scorpions.

Download Full-text

rDNA sequence analyses of Streptococcus dysgalactiae subsp. equisimilis isolates from pigs

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.02666-0 ◽

2003 ◽

Vol 53 (6) ◽

pp. 1941-1946 ◽

Cited By ~ 16

Author(s):

K. Kawata

Keyword(s):

Rdna Sequence ◽

Streptococcus Dysgalactiae ◽

Sequence Analyses

Download Full-text

Culture negative mitral valve endocarditis caused by Neisseria gonorrhoeae confirmed by 16S rDNA sequence analysis of resected valvular tissue

Journal of Cardiology Cases ◽

10.1016/j.jccase.2011.01.007 ◽

2011 ◽

Vol 3 (2) ◽

pp. e82-e85

Author(s):

Stuart J. Butterly ◽

David F.M. Looke ◽

Shane Byrne ◽

Gerry Kaye

Keyword(s):

Sequence Analysis ◽

Mitral Valve ◽

Neisseria Gonorrhoeae ◽

16S Rdna ◽

Rdna Sequence ◽

16S Rdna Sequence ◽

16S Rdna Sequence Analysis ◽

Culture Negative

Download Full-text

Rapid Detection, Identification, and Enumeration ofEscherichia coli Cells in Municipal Water by Chemiluminescent In Situ Hybridization

Applied and Environmental Microbiology ◽

10.1128/aem.67.1.142-147.2001 ◽

2001 ◽

Vol 67 (1) ◽

pp. 142-147 ◽

Cited By ~ 29

Author(s):

Henrik Stender ◽

Adam J. Broomer ◽

Kenneth Oliveira ◽

Heather Perry-O'Keefe ◽

Jens J. Hyldig-Nielsen ◽

...

Keyword(s):

In Situ Hybridization ◽

16S Rrna ◽

Sensitivity And Specificity ◽

Bacterial Species ◽

Rdna Sequence ◽

Sequence Information ◽

Sequence Alignments ◽

E Coli ◽

Municipal Water

ABSTRACT A new chemiluminescent in situ hybridization (CISH) method provides simultaneous detection, identification, and enumeration of culturableEscherichia coli cells in 100 ml of municipal water within one working day. Following filtration and 5 h of growth on tryptic soy agar at 35°C, individual microcolonies of E. coliwere detected directly on a 47-mm-diameter membrane filter using soybean peroxidase-labeled peptide nucleic acid (PNA) probes targeting a species-specific sequence in E. coli 16S rRNA. Within each microcolony, hybridized, peroxidase-labeled PNA probe and chemiluminescent substrate generated light which was subsequently captured on film. Thus, each spot of light represented one microcolony of E. coli. Following probe selection based on 16S ribosomal DNA (rDNA) sequence alignments and sample matrix interference, the sensitivity and specificity of the probe Eco16S07C were determined by dot hybridization to RNA of eight bacterial species. Only the rRNA of E. coli and Pseudomonas aeruginosa were detected by Eco16S07C with the latter mismatch hybridization being eliminated by a PNA blocker probe targetingP. aeruginosa 16S rRNA. The sensitivity and specificity for the detection of E. coli by PNA CISH were then determined using 8 E. coli strains and 17 other bacterial species, including closely related species. No bacterial strains other thanE. coli and Shigella spp. were detected, which is in accordance with 16S rDNA sequence information. Furthermore, the enumeration of microcolonies of E. coli represented by spots of light correlated 92 to 95% with visible colonies following overnight incubation. PNA CISH employs traditional membrane filtration and culturing techniques while providing the added sensitivity and specificity of PNA probes in order to yield faster and more definitive results.

Download Full-text