LYMPHOCYSTIS DISEASE VIRUS (IRIDOVIRIDAE)

1999 ◽  
pp. 908-911 ◽  
Author(s):  
Christian A. Tidona ◽  
Gholamreza Darai
Keyword(s):  
Disease Virus ◽  
Lymphocystis Disease Virus ◽  
Lymphocystis Disease

Detection of lymphocystis disease virus (LCDV) in asymptomatic cultured gilt-head seabream (Sparus aurata, L.) using an immunoblot technique

2006 ◽  
Vol 113 (1-2) ◽  
pp. 137-141 ◽  
Author(s):  
I. Cano ◽  
M.C. Alonso ◽  
E. Garcia-Rosado ◽  
S. Rodriguez Saint-Jean ◽  
D. Castro ◽  
...  
Keyword(s):  
Disease Virus ◽  
Sparus Aurata ◽  
Lymphocystis Disease Virus ◽  
Lymphocystis Disease ◽  
Immunoblot Technique

Induction of apoptosis in a flounder gill cell line by lymphocystis disease virus infection

2004 ◽  
Vol 27 (11) ◽  
pp. 657-662 ◽  
Author(s):  
G-B Hu ◽  
R-S Cong ◽  
T-J Fan ◽  
X-G Mei
Keyword(s):  
Virus Infection ◽  
Cell Line ◽  
Disease Virus ◽  
Lymphocystis Disease Virus ◽  
Gill Cell ◽  
Lymphocystis Disease ◽  
Induction Of Apoptosis

Effect of probiotics enriched diet on Paralichthys olivaceus infected with lymphocystis disease virus (LCDV)

2010 ◽  
Vol 29 (5) ◽  
pp. 868-874 ◽  
Author(s):  
Ramasamy Harikrishnan ◽  
Chellam Balasundaram ◽  
Moon-Soo Heo
Keyword(s):  
Disease Virus ◽  
Paralichthys Olivaceus ◽  
Lymphocystis Disease Virus ◽  
Lymphocystis Disease

Identification and characterization of a novel lymphocystis disease virus isolate from cultured grouper in China

2014 ◽  
Vol 38 (4) ◽  
pp. 379-387 ◽  
Author(s):  
X Huang ◽  
Y Huang ◽  
L Xu ◽  
S Wei ◽  
Z Ouyang ◽  
...  
Keyword(s):  
Disease Virus ◽  
Virus Isolate ◽  
Lymphocystis Disease Virus ◽  
Lymphocystis Disease ◽  
Identification And Characterization

Molecular anatomy of lymphocystis disease virus

1997 ◽  
pp. 49-56
Author(s):  
C. A. Tidona ◽  
G. Darai
Keyword(s):  
Disease Virus ◽  
Lymphocystis Disease Virus ◽  
Lymphocystis Disease ◽  
Molecular Anatomy

scholarly journals Complete genome sequence and analysis of a novel lymphocystivirus detected in whitemouth croaker (Micropogonias furnieri): lymphocystis disease virus 4

2020 ◽  
Vol 165 (5) ◽  
pp. 1215-1218
Author(s):  
Andor Doszpoly ◽  
Győző L. Kaján ◽  
Rodrigo Puentes ◽  
Alejandro Perretta
Keyword(s):  
Disease Virus ◽  
Complete Genome ◽  
Virus Disease ◽  
Sequence Similarity ◽  
Amino Acid Sequence Similarity ◽  
Lymphocystis Disease Virus ◽  
Protein Coding ◽  
Lymphocystis Disease ◽  
Micropogonias Furnieri ◽  
Core Genes

Abstract A novel lymphocystivirus causing typical signs of lymphocystis virus disease in whitemouth croaker (Micropogonias furnieri) on the coast of Uruguay was detected and described recently. Based on genetic analysis of some partially sequenced core genes, the virus seemed to differ from previously described members of the genus Lymphocystivirus. In this study, using next-generation sequencing, the whole genome of this virus was sequenced and analysed. The complete genome was found to be 211,086 bp in size, containing 148 predicted protein-coding regions, including the 26 core genes that seem to have a homologue in every iridovirus genome sequenced to date. Considering the current species demarcation criteria for the family Iridoviridae (genome organization, G+C content, amino acid sequence similarity, and phylogenetic relatedness of the core genes), the establishment of a novel species (“Lymphocystis disease virus 4”) in the genus Lymphocystivirus is suggested.

Proteomic aspects of infection by lymphocystis disease virus in Japanese flounder (Paralichthys olivaceus)

2011 ◽  
Vol 29 (3) ◽  
pp. 603-608
Author(s):  
Mingqing Xing ◽  
Xiuqin Sun ◽  
Fengrong Zheng ◽  
Lingyun Qu ◽  
Xuguang Hong ◽  
...  
Keyword(s):  
Disease Virus ◽  
Paralichthys Olivaceus ◽  
Japanese Flounder ◽  
Lymphocystis Disease Virus ◽  
Lymphocystis Disease

scholarly journals Complete Genome Sequence of Lymphocystis Disease Virus Isolated from China

2004 ◽  
Vol 78 (13) ◽  
pp. 6982-6994 ◽  
Author(s):  
Qi-Ya Zhang ◽  
Feng Xiao ◽  
Jian Xie ◽  
Zheng-Qiu Li ◽  
Jian-Fang Gui
Keyword(s):  
Amino Acid ◽  
Disease Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Computer Assisted ◽  
Gene Products ◽  
Lymphocystis Disease Virus ◽  
C Genome ◽  
Lymphocystis Disease

ABSTRACT Lymphocystis diseases in fish throughout the world have been extensively described. Here we report the complete genome sequence of lymphocystis disease virus isolated in China (LCDV-C), an LCDV isolated from cultured flounder (Paralichthys olivaceus) with lymphocystis disease in China. The LCDV-C genome is 186,250 bp, with a base composition of 27.25% G+C. Computer-assisted analysis revealed 240 potential open reading frames (ORFs) and 176 nonoverlapping putative viral genes, which encode polypeptides ranging from 40 to 1,193 amino acids. The percent coding density is 67%, and the average length of each ORF is 702 bp. A search of the GenBank database using the 176 individual putative genes revealed 103 homologues to the corresponding ORFs of LCDV-1 and 73 potential genes that were not found in LCDV-1 and other iridoviruses. Among the 73 genes, there are 8 genes that contain conserved domains of cellular genes and 65 novel genes that do not show any significant homology with the sequences in public databases. Although a certain extent of similarity between putative gene products of LCDV-C and corresponding proteins of LCDV-1 was revealed, no colinearity was detected when their ORF arrangements and coding strategies were compared to each other, suggesting that a high degree of genetic rearrangements between them has occurred. And a large number of tandem and overlapping repeated sequences were observed in the LCDV-C genome. The deduced amino acid sequence of the major capsid protein (MCP) presents the highest identity to those of LCDV-1 and other iridoviruses among the LCDV-C gene products. Furthermore, a phylogenetic tree was constructed based on the multiple alignments of nine MCP amino acid sequences. Interestingly, LCDV-C and LCDV-1 were clustered together, but their amino acid identity is much less than that in other clusters. The unexpected levels of divergence between their genomes in size, gene organization, and gene product identity suggest that LCDV-C and LCDV-1 shouldn't belong to a same species and that LCDV-C should be considered a species different from LCDV-1.

scholarly journals Development and Characterization of Monoclonal Antibodies to the 32 kDa Viral Attachment Protein of Lymphocystis Disease Virus and Their Neutralizing Ability in Vitro

2018 ◽  
Vol 19 (9) ◽  
pp. 2536 ◽  
Author(s):  
Ying Zhong ◽  
Xiaoqian Tang ◽  
Xiuzhen Sheng ◽  
Jing Xing ◽  
Wenbin Zhan
Keyword(s):  
Monoclonal Antibodies ◽  
Disease Virus ◽  
Paralichthys Olivaceus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dilution Method ◽  
Attachment Protein ◽  
Lymphocystis Disease Virus ◽  
Viral Attachment ◽  
Lymphocystis Disease

In previous research, a 32 kDa protein in lymphocystis disease virus (LCDV) was identified as viral attachment protein (VAP) that specifically interacted with the 27.8 kDa cellular receptor from flounder Paralichthys olivaceus gill (FG) cells, and the recombinant VAP (rVAP) was expressed in Escherichia coli strain BL21 (DE3). In this study, monoclonal antibodies (MAbs) against 32 kDa VAP are produced by immunization of BALB/c mice with the rVAP. Seven hybridoma secreting MAbs were screened by enzyme-linked immunosorbent assay, five of which designated as 1C6, 1C8, 3B5, 3D11 and 3H10 are cloned by the limiting dilution method, depending on the strongly positive results of ELISA. Western blotting analysis shows that the five MAbs can specifically react with the 32 kDa protein of LCDV and the purified 50 kDa rVAP, and the subtype of the MAbs is identified as IgG. Immunofluorescence results demonstrate that the specific fluorescence signals for LCDV appear in the cytoplasm of FG cells at 24 h post LCDV infection. Neutralization assay results indicate that pre-incubations of LCDV with the five MAbs can significantly decrease the LCDV copy numbers and delay the development of the cytopathic effect in FG cells, revealing that the five MAbs can neutralize the LCDV particles and block viral infection in vitro. The neutralizing MAbs against 32 kDa VAP would be useful for the study on the LCDV–host interaction and might be promising inhibitors of LCDV infection in fish.

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