scholarly journals RNA Synthesis by the Brome Mosaic Virus RNA-Dependent RNA Polymerase in Human Cells Reveals Requirements forDe NovoInitiation and Protein-Protein Interaction

2012 ◽  
Vol 86 (8) ◽  
pp. 4317-4327 ◽  
Author(s):  
Chennareddy V. Subba-Reddy ◽  
Brady Tragesser ◽  
Zhili Xu ◽  
Barry Stein ◽  
C. T. Ranjith-Kumar ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Mosaic Virus ◽  
Protein Interaction ◽  
Rna Synthesis ◽  
Brome Mosaic Virus ◽  
Human Cells ◽  
Rna Dependent Rna Polymerase ◽  
Protein Protein Interaction ◽  
Virus Rna

scholarly journals Use of DNA, RNA, and Chimeric Templates by a Viral RNA-Dependent RNA Polymerase: Evolutionary Implications for the Transition from the RNA to the DNA World

1999 ◽  
Vol 73 (8) ◽  
pp. 6424-6429 ◽  
Author(s):  
Robert W. Siegel ◽  
Laurent Bellon ◽  
Leonid Beigelman ◽  
C. Cheng Kao
Keyword(s):  
Rna Polymerase ◽  
Mosaic Virus ◽  
Rna Synthesis ◽  
Brome Mosaic Virus ◽  
Viral Rna ◽  
Competition Assay ◽  
Rna Dependent Rna Polymerase ◽  
Dna Molecule ◽  
Mechanism Of Catalysis ◽  
Insight Into

ABSTRACT All polynucleotide polymerases have a similar structure and mechanism of catalysis, consistent with their evolution from one progenitor polymerase. Viral RNA-dependent RNA polymerases (RdRp) are expected to have properties comparable to those from this progenitor and therefore may offer insight into the commonalities of all classes of polymerases. We examined RNA synthesis by the brome mosaic virus RdRp on DNA, RNA, and hybrid templates and found that precise initiation of RNA synthesis can take place from all of these templates. Furthermore, initiation can take place from either internal or penultimate initiation sites. Using a template competition assay, we found that the BMV RdRp interacts with DNA only three- to fourfold less well than it interacts with RNA. Moreover, a DNA molecule with a ribonucleotide at position −11 relative to the initiation nucleotide was able to interact with RdRp at levels comparable to that observed with RNA. These results suggest that relatively few conditions were needed for an ancestral RdRp to replicate DNA genomes.

scholarly journals Initiation of Genomic Plus-Strand RNA Synthesis from DNA and RNA Templates by a Viral RNA-Dependent RNA Polymerase

1999 ◽  
Vol 73 (8) ◽  
pp. 6415-6423 ◽  
Author(s):  
K. Sivakumaran ◽  
C. Cheng Kao
Keyword(s):  
Rna Polymerase ◽  
Mosaic Virus ◽  
Rna Synthesis ◽  
Mutational Analysis ◽  
Initiation Site ◽  
Brome Mosaic Virus ◽  
Minus Strand ◽  
Rna Dependent Rna Polymerase ◽  
Dna And Rna

ABSTRACT In contrast to the synthesis of minus-strand genomic and plus-strand subgenomic RNAs, the requirements for brome mosaic virus (BMV) genomic plus-strand RNA synthesis in vitro have not been previously reported. Therefore, little is known about the biochemical requirements for directing genomic plus-strand synthesis. Using DNA templates to characterize the requirements for RNA-dependent RNA polymerase template recognition, we found that initiation from the 3′ end of a template requires one nucleotide 3′ of the initiation nucleotide. The addition of a nontemplated nucleotide at the 3′ end of minus-strand BMV RNAs led to initiation of genomic plus-strand RNA in vitro. Genomic plus-strand initiation was specific since cucumber mosaic virus minus-strand RNA templates were unable to direct efficient synthesis under the same conditions. In addition, mutational analysis of the minus-strand template revealed that the −1 nontemplated nucleotide, along with the +1 cytidylate and +2 adenylate, is important for RNA-dependent RNA polymerase interaction. Furthermore, genomic plus-strand RNA synthesis is affected by sequences 5′ of the initiation site.

scholarly journals Characterization of a host protein associated with brome mosaic virus RNA-dependent RNA polymerase.

1993 ◽  
Vol 90 (4) ◽  
pp. 1498-1502 ◽  
Author(s):  
R. Quadt ◽  
C. C. Kao ◽  
K. S. Browning ◽  
R. P. Hershberger ◽  
P. Ahlquist
Keyword(s):  
Rna Polymerase ◽  
Mosaic Virus ◽  
Brome Mosaic Virus ◽  
Host Protein ◽  
Rna Dependent Rna Polymerase ◽  
Virus Rna

scholarly journals Heat shock 70 protein interaction with Turnip mosaic virus RNA-dependent RNA polymerase within virus-induced membrane vesicles

Virology ◽  
2008 ◽  
Vol 374 (1) ◽  
pp. 217-227 ◽  
Author(s):  
Philippe J. Dufresne ◽  
Karine Thivierge ◽  
Sophie Cotton ◽  
Chantal Beauchemin ◽  
Christine Ide ◽  
...  
Keyword(s):  
Heat Shock ◽  
Rna Polymerase ◽  
Mosaic Virus ◽  
Protein Interaction ◽  
Membrane Vesicles ◽  
Turnip Mosaic Virus ◽  
Rna Dependent Rna Polymerase ◽  
Induced Membrane ◽  
Virus Rna

scholarly journals Spatial Perturbations within an RNA Promoter Specifically Recognized by a Viral RNA-Dependent RNA Polymerase (RdRp) Reveal That RdRp Can Adjust Its Promoter Binding Sites

1999 ◽  
Vol 73 (1) ◽  
pp. 198-204 ◽  
Author(s):  
Scott Stevenson Stawicki ◽  
C. Cheng Kao
Keyword(s):  
Rna Polymerase ◽  
Rna Synthesis ◽  
Core Promoter ◽  
Brome Mosaic Virus ◽  
Viral Rna ◽  
Rna Dependent Rna Polymerase ◽  
Dna And Rna ◽  
In The Wild ◽  
Nucleotide Insertions

ABSTRACT RNA synthesis during viral replication requires specific recognition of RNA promoters by the viral RNA-dependent RNA polymerase (RdRp). Four nucleotides (−17, −14, −13, and −11) within the brome mosaic virus (BMV) subgenomic core promoter are required for RNA synthesis by the BMV RdRp (R. W. Siegel et al., Proc. Natl. Acad. Sci. USA 94:11238–11243, 1997). The spatial requirements for these four nucleotides and the initiation (+1) cytidylate were examined in RNAs containing nucleotide insertions and deletions within the BMV subgenomic core promoter. Spatial perturbations between nucleotides −17 and −11 resulted in decreased RNA synthesis in vitro. However, synthesis was still dependent on the key nucleotides identified in the wild-type core promoter and the initiation cytidylate. In contrast, changes between nucleotides −11 and +1 had a less severe effect on RNA synthesis but resulted in RNA products initiated at alternative locations in addition to the +1 cytidylate. The results suggest a degree of flexibility in the recognition of the subgenomic promoter by the BMV RdRp and are compared with functional regions in other DNA and RNA promoters.

Sign in / Sign up

Export Citation Format

Share Document