A Cytopathic and a Cell Culture Adapted Hepatitis A Virus Strain Differ in Cell Killing but Not in Intracellular Membrane Rearrangements

ABSTRACT Chimeras between human (HM-175) and simian (AGM-27) strains of hepatitis A virus (HAV) were constructed to evaluate the effect of the 2C gene of AGM-27 on HAV replication in cell culture and virulence in tamarins (Saguinus mystax) and chimpanzees (Pan troglodytes). Kinetic studies and radioimmunofocus assays demonstrated that replacement of the 2C gene of HAV/7, a cell culture-adapted strain of HM-175, with that of AGM-27 drastically reduced the ability of the virus to replicate in cultured cells. Intragenic chimeras containing AGM-27 sequences in either the 5′ or 3′ half of the 2C gene replicated in cell culture at an intermediate level. Whereas HAV/7 is attenuated for tamarins, a chimera containing the simian virus 2C gene in the HAV/7 background was virulent in tamarins, demonstrating that the simian virus 2C gene alone can confer the phenotype of virulence to an otherwise attenuated virus. Clusters of AGM-27-specific residues near both ends of the 2C protein were required for virulence since a chimera containing AGM-27 sequences in the carboxy-terminal half of 2C was partially attenuated for tamarins while one containing AGM-27 sequences only in the amino-terminal half of 2C was even more attenuated. Chimeras containing either the entire or only the 3′ half of the simian virus 2C gene in the HAV/7 background were attenuated for chimpanzees.

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Kinetic study of the replication of a cell-culture-adapted hepatitis A virus

Research in Virology ◽

10.1016/0923-2516(91)90005-n ◽

1991 ◽

Vol 142 (5) ◽

pp. 381-385

Author(s):

S. Zou ◽

R.K. Chaudhary

Keyword(s):

Cell Culture ◽

Kinetic Study ◽

Hepatitis A ◽

Hepatitis A Virus ◽

A Cell

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Attenuation Phenotype of a Cell Culture-Adapted Variant of Hepatitis A Virus (HM175/p16) in Susceptible New World Owl Monkeys

The Journal of Infectious Diseases ◽

10.1093/infdis/168.3.592 ◽

1993 ◽

Vol 168 (3) ◽

pp. 592-601 ◽

Cited By ~ 12

Author(s):

K. L. Taylor ◽

P. C. Murphy ◽

L. V. S. Asher ◽

J. W. LeDuc ◽

S. M. Lemon

Keyword(s):

Cell Culture ◽

Hepatitis A ◽

New World ◽

Hepatitis A Virus ◽

A Cell ◽

Owl Monkeys

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Nucleotide Sequence of High-passage Hepatitis A Virus Strain HM175: Comparison with Wild-type and Cell Culture-adapted Strains

Journal of General Virology ◽

10.1099/0022-1317-70-10-2805 ◽

1989 ◽

Vol 70 (10) ◽

pp. 2805-2810 ◽

Cited By ~ 13

Author(s):

B. C. Ross ◽

B. N. Anderson ◽

P. C. Edwards ◽

I. D. Gust

Keyword(s):

Cell Culture ◽

Nucleotide Sequence ◽

Virus Strain ◽

Hepatitis A ◽

Hepatitis A Virus ◽

Wild Type ◽

High Passage

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Hepatitis a Virus Concentration from Experimentally Contaminated Distilled, Tap, Waste and Seawater

Water Science & Technology ◽

10.2166/wst.1989.0112 ◽

1989 ◽

Vol 21 (3) ◽

pp. 255-258 ◽

Cited By ~ 4

Author(s):

Evangélos Biziagos ◽

Jacques Passagot ◽

Jean-Marc Crance ◽

Robert Deloince

Keyword(s):

Cell Culture ◽

Polyethylene Glycol ◽

Hepatitis A ◽

Hepatitis A Virus ◽

Tap Water ◽

Virus Concentration ◽

Beef Extract ◽

Polyethylene Glycol 6000

The concentration of cell-culture-adapted hepatitis A virus (HAV) from experimentally contaminated distilled, drinking, waste and seawater was performed by using a filter adsorption-elu-tion method in the following conditions: HAV seeded in water was adsorbed at pH 4.0 to two nitrocellulose membranes (1.2 and 0.45 µm porosity for distilled and tap water or 8.0 and 3.0 µm porosity for waste and seawater), then eluted by 3% beef-extract at pH 8.5 and further concentrated by polyethylene glycol 6000 precipitation. Thus, HAV in 5 to 50 liters of seeded waters was concentrated approximately 1,700 to 17,000 fold with greater than 70% recovery of the initial virus added to the samples.

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