Nonradioactive In Situ Hybridization in Combination with Tract-Tracing

2006 ◽  
pp. 237-262 ◽  
Author(s):  
Ruth L. Stornetta ◽  
Patrice G. Guyenet
Keyword(s):  
In Situ Hybridization ◽  
Tract Tracing ◽  
Nonradioactive In Situ Hybridization

Nonradioactive in Situ Hybridization with Digoxigenin Labeled DNA Probes

1992 ◽  
Vol 67 (2) ◽  
pp. 59-67 ◽  
Author(s):  
N. Arnold ◽  
R. Seibl ◽  
C. Kessler ◽  
J. Wienberg
Keyword(s):  
In Situ Hybridization ◽  
Dna Probes ◽  
Nonradioactive In Situ Hybridization

scholarly journals Freeze-drying Allows Double Nonradioactive ISH and Antigenic Labeling

2000 ◽  
Vol 48 (4) ◽  
pp. 499-507 ◽  
Author(s):  
Huguette Louis ◽  
Julie Lavie ◽  
Patrick Lacolley ◽  
Danièle Daret ◽  
Jacques Bonnet ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Freeze Drying ◽  
Simultaneous Detection ◽  
Tissue Preservation ◽  
Reliable Technique ◽  
Mrna Detection ◽  
Nonradioactive In Situ Hybridization

Because tissue freeze-drying is an excellent way to preserve antigenic conformation, we have tested the feasibility of this technique to reveal nonradioactive in situ hybridization (ISH) of tissue mRNA. We have compared mRNA detection after different methods of tissue preservation, freeze-drying, cryosectioning, and formaldehyde or methanol fixation. Our results show that nonradioactive ISH is more sensitive for tissues preserved by freeze-drying than for other tissue preparations. We have demonstrated that freeze-drying allows combination of ISH and immunohistochemistry for simultaneous detection of mRNA and antigen because with this technique of tissue preservation ISH does not affect the sensitivity or the amount of the detected antigens. This work underscores the fact that tissue freeze-drying is an easy, convenient, and reliable technique for both ISH and immunohistochemistry and achieves excellent structural conditions for nonradioactive detection.

A simple G-banding technique following nonradioactive in situ hybridization

1992 ◽  
Vol 63 (2) ◽  
pp. 107
Author(s):  
Lisa M. Duncan ◽  
Erin Dietzsch ◽  
Christina Rudduck ◽  
O. Margaret Garson
Keyword(s):  
In Situ Hybridization ◽  
Banding Technique ◽  
Nonradioactive In Situ Hybridization
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