Studying Cell–Surface Interactions In Vitro: A Survey of Experimental Approaches and Techniques

2011 ◽  
pp. 33-66 ◽  
Author(s):  
Stefanie Michaelis ◽  
Rudolf Robelek ◽  
Joachim Wegener
Keyword(s):  
Cell Surface ◽  
Surface Interactions ◽  
Experimental Approaches ◽  
Cell Surface Interactions

scholarly journals Osteosphere Model to Evaluate Cell–Surface Interactions of Implantable Biomaterials

Materials ◽  
2021 ◽  
Vol 14 (19) ◽  
pp. 5858
Author(s):  
Ana Carolina Batista Brochado ◽  
Victor Hugo de Souza ◽  
Joice Correa ◽  
Suzana Azevedo dos Anjos ◽  
Carlos Fernando de Almeida Barros Mourão ◽  
...  
Keyword(s):  
Cell Surface ◽  
3D Model ◽  
Bone Cells ◽  
Three Dimensional ◽  
Surface Interactions ◽  
Force Microscopy ◽  
Tissue Therapy ◽  
Cell Surface Interactions ◽  
Titanium Surfaces

Successful biomaterials for bone tissue therapy must present different biocompatible properties, such as the ability to stimulate the migration and proliferation of osteogenic cells on the implantable surface, to increase attachment and avoid the risks of implant movement after surgery. The present work investigates the applicability of a three-dimensional (3D) model of bone cells (osteospheres) in the evaluation of osteoconductive properties of different implant surfaces. Three different titanium surface treatments were tested: machined (MA), sandblasting and acid etching (BE), and Hydroxyapatite coating by plasma spray (PSHA). The surfaces were characterized by Scanning Electron Microscopy (SEM) and atomic force microscopy (AFM), confirming that they present very distinct roughness. After seeding the osteospheres, cell–surface interactions were studied in relation to cell proliferation, migration, and spreading. The results show that BE surfaces present higher densities of cells, leaving the aggregates towards than titanium surfaces, providing more evidence of migration. The PSHA surface presented the lowest performance in all analyses. The results indicate that the 3D model allows the focal analysis of an in vitro cell/surfaces interaction of cells and surfaces. Moreover, by demonstrating the agreement with the clinical data observed in the literature, they suggest a potential use as a predictive preclinical tool for investigating osteoconductive properties of novel biomaterials for bone therapy.

scholarly journals Significance of Nano- and Microtopography for Cell-Surface Interactions in Orthopaedic Implants

2007 ◽  
Vol 2007 ◽  
pp. 1-19 ◽  
Author(s):  
M. Jäger ◽  
C. Zilkens ◽  
K. Zanger ◽  
R. Krauspe
Keyword(s):  
Cell Surface ◽  
Cell Types ◽  
Surface Interactions ◽  
Orthopaedic Implants ◽  
Nanostructured Surfaces ◽  
Major Interest ◽  
Cell Surface Interactions ◽  
Biomaterial Application ◽  
Different Cell Types

Cell-surface interactions play a crucial role for biomaterial application in orthopaedics. It is evident that not only the chemical composition of solid substances influence cellular adherence, migration, proliferation and differentiation but also the surface topography of a biomaterial. The progressive application of nanostructured surfaces in medicine has gained increasing interest to improve the cytocompatibility and osteointegration of orthopaedic implants. Therefore, the understanding of cell-surface interactions is of major interest for these substances. In this review, we elucidate the principle mechanisms of nano- and microscale cell-surface interactions in vitro for different cell types onto typical orthopaedic biomaterials such as titanium (Ti), cobalt-chrome-molybdenum (CoCrMo) alloys, stainless steel (SS), as well as synthetic polymers (UHMWPE, XLPE, PEEK, PLLA). In addition, effects of nano- and microscaled particles and their significance in orthopaedics were reviewed. The significance for the cytocompatibility of nanobiomaterials is discussed critically.

scholarly journals Endogenous hyaluronate-cell surface interactions in 3T3 and simian virus-transformed 3T3 cells.

1984 ◽  
Vol 259 (15) ◽  
pp. 9440-9446
Author(s):  
R L Goldberg ◽  
J D Seidman ◽  
G Chi-Rosso ◽  
B P Toole
Keyword(s):  
Cell Surface ◽  
Surface Interactions ◽  
Simian Virus ◽  
3T3 Cells ◽  
Cell Surface Interactions

Stimuli-responsive interfaces and systems for the control of protein–surface and cell–surface interactions

Biomaterials ◽  
2009 ◽  
Vol 30 (9) ◽  
pp. 1827-1850 ◽  
Author(s):  
Martin A. Cole ◽  
Nicolas H. Voelcker ◽  
Helmut Thissen ◽  
Hans J. Griesser
Keyword(s):  
Cell Surface ◽  
Surface Interactions ◽  
Stimuli Responsive ◽  
Protein Surface ◽  
Cell Surface Interactions

Cell Surface Interactions with Concanavalin A: Determination by Microhemadsorption

1972 ◽  
Vol 140 (1) ◽  
pp. 216-219 ◽  
Author(s):  
P. Furmanski ◽  
P. G. Phillips ◽  
M. Lubin
Keyword(s):  
Cell Surface ◽  
Concanavalin A ◽  
Surface Interactions ◽  
Cell Surface Interactions
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